...and high-titer viral preparations of plasmids that are currently in our repository. ID Plasmid Description...sites AAV Uchida Cre Reporters and Tools In this subtype of loxP plasmids, reporter genes indicate which...Mammalian Pelczar 62732 Cre Reporter DsRED and EGFP Cre recombinase reporter Lentiviral Geijsen 65726 pLV-CMV-LoxP-DsRed-LoxP-eGFP...Vectors Cre-containing Plasmids loxP Constructs Cre Reporters & Tools Additional Resources References Background...Fluorescent Cre: The fusion of Cre to a fluorescent reporter enables visualization of Cre expression. Optimized... of choice. We also offer ready-to-use viral preparations of select Cre expression plasmids. Addgene's...HRE:GFP-pGK:Cre Cre and hypoxia-responsive GFP reporter HRE Lentiviral Jacks 69570 pAAV-EF1a-N-CretrcintG...

...Plasmid Description PI Viral Preps Addgene distributes ready-to-use viral preps made from plasmids in our... BRAIN Initiative collection of plasmids, viral preps, and antibodies here. Collections...the research community. Browse plasmids , viral preps , and antibodies created with support from the BRAIN...use. Learn more about our viral service ID Viral Prep Description PI 65417-AAV8 pAAV-hSyn-dF-HA-KORD-IRES-mCitrine...plasmid encoding for Archon1 fluorescent voltage reporter, Cre-dependent expression Edward Boyden 108912...Expresses the genetically-encoded fluorescent norepinephrine(NE) sensor GRAB_NE1m in neurons Yulong Li 123308...Expresses the genetically-encoded fluorescent norepinephrine(NE) sensor GRAB_NE1m in neurons Yulong Li 123309...

...Envelope and Packaging Plasmids Reporter Plasmids A few examples of reporter plasmids that can be used for... Lentiviral reporter vector expressing firefly luciferase. pCMV-FLuc - Retroviral reporter vector expressing...vesicular stomatitis virus G) are packaged as replication-defective virus particles with the SARS-CoV-2...available in standard format and as a Lentiviral prep . ID Plasmid Description Mutations Industry PI Due...pHAGE-CMV-Luc2-IRES-ZsGreen-W - Lentiviral dual reporter vector expressing firefly luciferase and ZsGreen...ZsGreen. NL4-3 mCherry Luciferase - Lentiviral dual reporter vector expressing mCherry and firefly luciferase...destabilized EGFP. Lenti-luciferase-P2A-Neo - Lentiviral reporter vector expressing firefly luciferase with neomycin...

.... When a repair template is present, the cell may repair a DSB using homology-directed repair (HDR) instead...be used with a repair template to introduce specific edits via homology-directed repair (HDR). ID Plasmid...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The...gRNA-defined target sequence. DSBs are preferentially repaired in the cell by non-homologous end joining (NHEJ...genomic changes, researchers use ssDNA or dsDNA repair templates with homology to the DNA flanking the...nicks create a double-strand break (DSB) that is repaired using error-prone non-homologous end joining (...

...amino acid repeats. These repeats only differ from each other by two amino acids, their repeat-variable ... of 18 TAL effector repeats. The kit can be used to make arrays from 12-31 repeats. Perhaps one of the... to this DNA targeting ideal. Figure 1: (A) Representation of a zinc finger (ZF) nuclease. Zinc fingers...if not slightly lower. Figure 2: Simplified representation of the Voytas/Bogdanove Golden Gate TALEN kit... would expect that assembling relatively small, repeat-laden DNA regions into a single construct would...from Addgene , custom arrays consisting of 12-31 repeats can be assembled and inserted into a variety of...

...- and serine-containing peptide linker (GGSGGS) repeat pET28CLY2 Peptide linker standard consisting of...of ECFP and EYFP connected by 2 GGSGGS repeats pET28CLY3 Peptide linker standard consisting of ECFP and...and EYFP connected by 3 GGSGGS repeats pET28CLY4 Peptide linker standard consisting of ECFP and EYFP connected... connected by 4 GGSGGS repeats pET28CLY5 Peptide linker standard consisting of ECFP and EYFP connected...connected by 5 GGSGGS repeats pET28CLY6 Peptide linker standard consisting of ECFP and EYFP connected by 6 GGSGGS...GGSGGS repeats pET28CLY7 Peptide linker standard consisting of ECFP and EYFP connected by 7 GGSGGS repeats...consisting of ECFP and EYFP connected by 8 GGSGGS repeats pET28CLY9 Peptide linker standard consisting of...

...tyrS gene knocked out, tyrTV gene replaced by tolC, and tyrU gene replaced by GentR). Expresses archaeal ...tyrTV gene replaced by tRNA variant that suppress the TAG nonsense codon, and tyrU gene replaced by tRNA ...tyrS gene knocked out, tyrTV gene replaced by tolC, and tyrU gene replaced by GentR) and release factor 1...biopolymers. Tips for Success Before beginning to reprogram the genetic code, there are several things to ... are true. Always express first with a control reporter gene – GFP for E. coli or mCherry-GFP for mammalian...RF1 function removed, with Ubiquitin-UAG-sfGFP reporter George Church 98565 C321.ΔClpS.Ub-UAG-sfGFP all...removed, ClpS inactivated, with Ubiquitin-UAG-sfGFP reporter George Church 174513 Syn61 No TCG, TCA, or TAG...

...Selectable markers Viral expression and packaging Reporters, shRNA expression, transgenics and genome...host organism. Host Relevant Promoters Representative Empty Backbones Mammalian CMV, SV40, EF1a, ...plasmid series - Expression with Strep, His or optimized YFP tags for purification...Tag or Fusion Protein Common uses Representative Empty Backbones Flag Epitope tag... Marker Typical Host Organism Representative Empty Backbones Neomycin (G418) ...methods: Delivery method Advantages Representative Empty Backbones Lentiviral Can transduce... AdEasy®-1 plasmid Back to Top Reporters, shRNA expression, transgenics and genome ...

...modifications: replacement of the Cbh promoter with EF1alpha addition of an mCherry reporter variant nuclear...utilizes a CRISPR/Cas9 based approach to knockout, repress, and activate gene targets. To accomplish this ...advantage of the Zhang lab all-in-one (guide/Cas9/reporter) pX458 plasmid family. They have made several ...nuclear reporters via an H2B fusion shuttle plasmid for shuttling U6-guide cassettes to make dual guide ...fusion for gene and non-coding enhancer sequence repression dCas9-VPR fusion for gene activation CRISPR/Cas9...175573 and 175574 express dCas9-KRAB-MeCP2 for repression. Plasmids 175571 and 175572 express dCas9-VPR...

...2~3-fold repress on targeted fluorescent genes. These plasmids are described in: Repurposing CRISPR as...Design Tools CRISPR Blog Posts We have recently repurposed the bacterial immune system, CRISPR ( C lustered...pathway, as an RNA-guided DNA binding platform, to repress expression of arbitrary genes in bacteria or human...efficiently block transcription (in bacteria, ~300-fold repression) by interfering with transcriptional elongation...plasmids in bacteria could cause up to 300-fold repress on targeted genes. 2. Two-plasmid CRISPRi system...Lentiviral vector that contains sgRNA targeting repetitive sequence of human MUC4 exon 3 46915 pU6-sgGAL4...

.... When a repair template is present, the cell may repair a DSB using homology-directed repair (HDR) instead...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Fully...gRNA-defined target sequence. DSBs are preferentially repaired in the cell by non-homologous end joining (NHEJ...genomic changes, researchers use ssDNA or dsDNA repair templates with 1. homology to the DNA flanking ...

.... When a repair template is present, the cell may repair a DSB using homology-directed repair (HDR) instead...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The...gRNA-defined target sequence. DSBs are preferentially repaired in the cell by non-homologous end joining (NHEJ...genomic changes, researchers use ssDNA or dsDNA repair templates with homology to the DNA flanking the...

.... When a repair template is present, the cell may repair a DSB using homology-directed repair (HDR) instead...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Eukaryotic...gRNA-defined target sequence. DSBs are preferentially repaired in the cell by non-homologous end joining (NHEJ...genomic changes, researchers use ssDNA or dsDNA repair templates with homology to the DNA flanking the...

...activity reporter of SARS-CoV-2 main protease Mpro. (Unpublished) A FlipGFP-based activity reporter of SARS-CoV... also lists several luciferase and fluorescent reporter plasmids that have been used for measuring viral...method termed Specific High Sensitivity Enzymatic Reporter UnLOCKING (SHERLOCK and SHERLOCKv2). The Broad... termed DNA Endonuclease Targeted CRISPR Trans Reporter (DETECTR). Mammoth Biosciences has published information...(Link opens in a new window) CRISPR tools and reporters now available from Stanley Qi's lab. COVID-19 ... Coronavirus Resource Hub COVID-19 SARS-CoV-2 preprints from medRxiv and bioRxiv Elsevier's Novel Coronavirus...

.... When a repair template is present, the cell may repair a DSB using homology-directed repair (HDR) instead...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The...gRNA-defined target sequence. DSBs are preferentially repaired in the cell by non-homologous end joining (NHEJ...genomic changes, researchers use ssDNA or dsDNA repair templates with homology to the DNA flanking the...

...to generate high quality preparations. Following production, all antibody preps are titered and undergo...Addgene does not recommend repeated freeze thaw of antibodies and suggests preparing single-use aliquots before...Innovation Antibody Collection webpage. Antibody preparations developed at IPI may differ from Addgene antibodies...Sanger Sequencing Purified recombinant antibody preps contain low levels of co-purified plasmid from transfection...

...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts CRISPR...Next-Generation Sequencing (NGS) to confirm library representation. CRISPR screening experiments require large...whether they are used to knockout, activate or repress target genes. Number of gRNAs - A given CRISPR ... taurus ) Library Size Genome-wide Subpool Viral Prep Available Yes No Clear Filters Name ID Library Type...Generation gRNAs per gene Total gRNAs Adamson DNA Repair CRISPRi Libraries 177663, 177664, 177670 Inhibition...A - The protospacers contained in the library represent all 4096 (4 6 ) combinations of 6 nucleotides....Editing Human Wei 3rd 3 53,502 sgRNA Pool for Streptococcus pneumoniae 170432 Knockout S. pneumoniae Veening...

...Mammalian expression vectors for TAL effector repeat arrays assembled using the REAL, REAL-Fast or FLASH... into which DNA fragments encoding TAL effector repeat arrays (assembled using the REAL, REAL-Fast or .... All three of these methods yield TAL effector repeat arrays with identical DNA and amino acid sequences...together with the Joung Lab Individual REAL TALE Repeat Plasmids in the Joung Lab REAL Assembly TALEN Kit...Biotechnol. 2011 ): ID Plasmid C-terminal 0.5 TALE repeat Binds 32285 JDS70 NI RVD A 32287 JDS71 HD RVD C...

...has been used for dynamic tracking of repetitive and non-repetitive genomic loci, as well as chromosome ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Using...given region. For example, chromosome-specific repetitive loci can be efficiently visualized in living ... targeted sequences in close proximity. A non-repetitive genomic locus can also be labeled by co-delivering...

...plasmids, the cell may instead repair the break using homology-directed repair (HDR). HDR is much less error-prone...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The...gRNA-defined target sequence. DSBs are preferentially repaired in the cell by non-homologous end joining (NHEJ...the DNA, possibly resulting in frameshifts. If a repair template with high homology to the DNA surrounding...

...generating dsDNA donors for homology-directed repair to replace genes or other genomic sequence with an attP...coinjected to generate mutations via imperfectly repaired DSBs. Coinjection of phsp70-Cas9 and plasmids ... of an ssODN donor template can mediate gene replacement by homologous recombination. For the sgRNA, vector...for generating dsDNA donors for homology-directed repair. Contains the visible marker 3xP3-DsRed....

...developed three platforms for engineering TAL effector repeat arrays of any desired length: the REAL (Restriction... three of these platforms assemble TAL effector repeat arrays on a single architectural framework and ...methods are identical in sequence. TAL effector repeat arrays produced on this framework has yielded TALENs...Lab REAL Assembly TALEN Kit Individual REAL TALE Repeat Plasmids TALEN Expression Vectors for REAL, REAL-Fast...for Endogenous Human Genes TALENs for the EGFP Reporter Gene Additional Plasmids compatible with the REAL...

...limited use label licenses (LULLs). Titer For AAV preps produced through our Packaged on Request service... we may expect lower titers for a particular AAV prep, mostly likely due to one of the following reasons...moving forward with a helpful solution. You must reply to that email within 10 business days in order to...been completed at the time of shipment. If any discrepancies are identified during this analysis, you will...moving forward with a helpful solution. You must reply to this email within 10 business days in order to...

...which is not required for the transcription or replication of the genome within infected cells, but is required... cells that lack the G protein, the virus can replicate but the newly formed viruses are unable to infect...starter cell via the EnvA receptor. RABV is able to replicate in the starter cell using the G-protein, which...eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our form to let us know what...what you would like to see available in viral prep format. Please note this does not guarantee viral vector...

...editing, empty backbones, fluorescent proteins, reporters, biosensors, and Cre expression plasmids. Large...studying diseases, are listed below: Lipid droplet reporters - Richard White Lab. An imaging platform to study...using red light. LipoGlo - Steven Farber Lab. A reporter system that allows direct assessment of lipoprotein...International Resource Center (ZIRC) - A US-based repository for zebrafish strains and materials and information...European Zebrafish Resource Center (EZRC) - A repository at the Karlsruhe Institute of Technology (KIT...

...leucine-rich repeat-containing G protein-coupled receptor 4 GPR48 LGR5 leucine-rich repeat-containing G... to the formation of a high avidity BCR through repeat cycles of expansion. Chemokines Chemokines, or ... to the formation of a high avidity BCR through repeat cycles of expansion. Symbol Name Synonyms AKT1 ...MGC102867 SEMA5A sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain ... semF SEMA5B sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain ...LGR1, MGC141667, MGC141668, ODG1 GAL galanin prepropeptide GALN, GLNN, GMAP, MGC40167 GALP galanin-like...GHRFR, GHRHRpsv, GRFR GHRL ghrelin/obestatin prepropeptide MTLRP, obestatin GHSR growth hormone secretagogue...

...be used with a repair template to introduce specific edits via homology-directed repair (HDR). Want more...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts CRISPR...nicks create a double-strand break (DSB) that is repaired using error-prone non-homologous end joining (...

... modification, including shuttle, integration, reporter, and tagging vectors. Metabolism Browse plasmids...regulatory elements, including promoters, terminators, repressors, activators, and more. Also contains pre-assembled...BioBrick Box Mukhopadhyay Lab Efflux Pumps Voigt Lab Repressors , Terminators , Light Signaling , Sigma Factors...Foundation Registry of Standard Biological Parts - Repository of community-created BioBricks BioBricks Foundation...

...Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Prime editing is a “search and replace” gene...Find prime editor plasmids for (search and replace) genome editing. CRISPR...heteroduplex is resolved by the cell’s mismatch repair (MMR) system. Alternatively, if the edited 3’ flap...

...Melina Fan/Leila Haery 5:00 PM Adjourn Meeting Preparation For Speakers: Each speaker will give a 20 minute... reagents)? For All Attendees: To help us best prepare and compile resources for this meeting's publication...meeting will be an openly shared communication representing a reference for AAV use in neuronal intersectional...commitment to open science! Please see the meeting preparation section for how to best help contribute to this...

...Microbiology Plasmids and Resources Addgene’s repository contains plasmid tools for a range of microbial...distributes cannot be used to reconstitute self-replicating microbes or recreate the diseases they cause....Salmonella sp. Shigella sp. Staphylococcus sp. Streptococcus sp. Synechococcus sp. Thermus sp. Vibrio sp....plasmid tools for the community that aren't in the repository? Help us widen our resources for the community...

...Biosafety Blog: Tips for Titering Your Lentiviral Preps Lentiviral Pooled Libraries All Viral Plasmids Packaging...Expresses shRNA under mouse U6 promoter; CMV-EGFP reporter cassette is included to monitor expression Parijs...Expresses shRNA under mouse U6 promoter; CMV-EGFP reporter cassette is included to monitor expression Kissler...transgene and CMV drives expression of RFP as a reporter. See plasmid 17618 for GFP plasmid. Cheng 17452...

...populations. For more information, see the bioRxiv preprint describing ICE . Deep Sequencing Data Analysis...non-homologous end joining (NHEJ) and homologous directed repair (HDR) frequency. CRISPResso requires two inputs...for genome editing, as well as gene regulation (repression and activation). Genome support for bacteria ... lab: Tips for CRISPR design, homology directed repair and cloning . CRISPR Discussion Group (moderated...

... engineered small proteins derived from ankyrin repeat proteins. These are antibody mimics instead of ...isotype. Overview of application Fix your samples. Prepare your samples using permeabilization, antigen retrieval...and controls follow that of IF (above). Sample preparation, signaling excitation, and assay readout vary...must also be run. Unique samples (non-biological replicates) often each require their own set of controls...conjugating them in the lab? How will the sample be prepped, and will this or other factors affect epitope ...

...bound to DNA, and the break can be repaired by the cell’s endogenous repair mechanisms. Cas3 belongs to the...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Cas3...

...and innovation programme and EFPIA. Figure 1: Representation of RESOLUTE mission. The combination of metabolomics...deorphanizing engine. Genetic and proteomic interactions represent the framework that provides biological context...the Resolute Partnership using their RESOLUTE repository feedback form . If you have further questions...

...Serotype Testing AAV These AAV encode fluorescent reporters and can be used to compare the tropism of different...eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our form to let us know what...what you would like to see available in viral prep format. Please note this does not guarantee viral vector...

...Endogenous Zebrafish Gene Targets Recent published reports have shown that engineered zinc finger nucleases...greater in a well-characterized bacterial two-hybrid reporter system but have NOT yet been tested for activity...OZ552 OPEN OPEN gGTCATCTGCcattcGAGGCGGACa REST corepressor 2 OZ553 and OZ554 OPEN OPEN gCTCACCATCtgttggaTGCGATGGAa...

...elegans genome is well studied and hundreds of reporters have been developed and characterized which allow...plasmid tools for the community that aren't in the repository? Help us widen our resources for the community...Contains a set of plasmids for building homologous repair templates that incorporate a self-excising drug...

...164499 EZH2-mEGFP AICSDP-83 mEGFP EZH2 Polycomb repressive complex 2 164500 POLR2A-mEGFP AICSDP-117 mEGFP...II 168798 TERF2-mEGFP AICSDP-77 mEGFP Telomeric repeat-binding factor 2 (TRF2) Telomeres 168799 CTCF-mEGFP...Proliferating cell nuclear antigen (PCNA) DNA replication foci IPSC cell lines can be ordered from the ...