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Showing: 1 - 20 of 32 results

  1. Cre-lox system

    Type
    Collection
    ...Scholer 125574 pCAG-Cre Cre CAG Mammalian Imai 125577 pCAG-iCre iCre CAG Mammalian Imai 125748 pCAG-sfGFP-GSAx9...Cre Bacterial Rajewsky 13775 pCAG-Cre Cre-Myc CAG Mammalian Cepko 13776 pCAG-Cre:GFP Cre-GFP fusion CAG ...Lentiviral Fuchs 26646 pCAG-Cre-IRES2-GFP Cre and GFP coexpression CAG Mammalian Chenn 26647 pCAG-Cre Cre CAG Mammalian...51267 pCAG-Co-InCreN N-terminal component of the Co-InCre system CAG Mammalian Pelczar 51268 pCAG-Co-InCreC...69572 pCAG-N-CretrcintG Cre recombinase dependent on GFP (CRE-DOG) CAG Mammalian Cepko 69573 pCAG-C-CreintG...Pu 87694 pCAG-ERT2-PhoCl-Cre-PhoCl-ERT2 light-activated Cre cTnT Mammalian Campbell 89573 pCAG-iCre iCre...Mammalian Heller 112618 pCAG-VHC Venus, Cre-ERT2 CAG Mammalian Heller 112619 pCAG-EHC Emerald, Cre-ERT2 ...

  2. Zhang Lab CRISPR Page

    Type
    Collection
    ...efficiency. SpCas9 and SpCas9n with 2a-Puro and 2a-EGFP are also available. 2. SpCas9 (or SpCas9n, D10A nickase...plasmid expressing SpCas9 alone without sgRNA. Full references are below. 1. SpCas9 (or SpCas9n, D10A nickase...expression cassettes, a human codon-optimized SpCas9 or SpCas9n, and the single guide RNA. The vector can ... 3. SpCas9 alone: This plasmid contains a single cassette expressing human codon-optimized SpCas9; sgRNA...codon-optimized SpCas9 plasmids can be ordered via the links below: 42230 : PX330; SpCas9 and single guide...guide RNA 48138 : PX458; SpCas9-2A-EGFP and single guide RNA 62988 : PX459; SpCas9-2A-Puro and single guide...RNA 48873 : PX460; SpCas9n (D10A nickase) and single guide RNA 48140 : PX461; SpCas9n-2A-EGFP (D10A nickase...

  3. CRISPR Guide

    Type
    Collection
    ...pyogenes (SP); SpCas9 3' NGG SpCas9 D1135E variant 3' NGG (reduced NAG binding) SpCas9 VRER variant 3'...NGCG SpCas9 EQR variant 3' NGAG SpCas9 VQR variant 3' NGAN or NGNG xCas9 3' NG, GAA, or GAT SpCas9-NG 3...engineered Cas9s can enhance CRISPR specificity. SpCas9 (from Streptococcus pyogenes ) is the most popular...no cuts. Cas9 nickase (Cas9n), a D10A mutant of SpCas9, has one active nuclease domain and one inactivated...fidelity enzymes, and their mechanisms, include: eSpCas9(1.1) - weaken interactions between the HNH/RuvC...RuvC groove and the non-target DNA strand SpCas9-HF1 - disrupt Cas9’s interactions with DNA phosphate backbone...your desired genes for modification. Engineered SpCas9 enzymes with altered PAM specificities are available...

  4. Adeno-associated virus (AAV) Plasmids

    Type
    Collection
    ...tTA-TRE amplification system Gradinaru 110770 pRepCap6 AAV6 pRepCap6 contains both rep and cap genes from the...KanR AAV-BI28 RepCap for AAV production Deverman 203533 iCAP-BI-48-KanR AAV-BI48 RepCap for AAV production...KanR AAV-BI49 RepCap for AAV production Deverman 203535 iCAP-BI-62-KanR AAV-BI62 RepCap for AAV production...plasmid (encoding adenovirus E4, E2A and VA) and a RepCap plasmid (encoding AAV Rep and respective capsid...packaging, expressing adenovirus E4, E2A and VA Wilson RepCap Plasmids for Serotypes Available as a Viral Service...controlled by tTA-TRE amplification system Gradinaru RepCap Plasmids for Serotypes Not Available as a Viral...production Deverman 203536 iCAP-BI-65-KanR AAV-BI65 RepCap for AAV production Deverman 205991 AAV2/9 REP-AAP-ΔCAP...

  5. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...Mb Cpf1 Kim pCAG-SpCas9-GFP-U6-gRNA 79144 Mammalian hU6 yes, cut S. pyogenes EGFP Zou pCAG-eCas9-GFP-U6...Chimeric_BB-CBh-hSpCas9-hGem(1/110) 71707 Mammalian hU6 yes, cut S. pyogenes Draetta pCAS1yl 73226 Other/...BsaI none S. pyogenes Chloramphenicol Marraffini pCas9 42876 Bacteria BsaI yes, cut S. pyogenes Chloramphenicol...backbone 61424 Mammalian BbsI none S. pyogenes Zhang pSpCas9n(BB)-2A-Puro (PX462) V2.0 62987 Mammalian BbsI ...BbsI yes, nick S. pyogenes Puro Zhang pSpCas9(BB)-2A-Puro (PX459) V2.0 62988 Mammalian BbsI yes, cut S. pyogenes...Yeast RPR1(TetO) yes, cut S. pyogenes URA3 Davis eSpCas9(1.1) 71814 Mammalian U6 yes, cut (enhanced) S. ...

  6. TALEN Plasmids and Kits

    Type
    Collection
    ...Golden Gate TALEN 2.0 37184 pCAG-T7-TALEN(Sangamo)-Destination Pawel Pelczar pCAG-T7-TALEN(Sangamo)-Destination...mutations) FokI domains. 40131 pCAG-T7-TALEN(Sangamo)-FokI-KKR-Destination 40132 pCAG-T7-TALEN(Sangamo)-FokI-...robust cleavage activity in several later studies. pCAG-T7-TALEN(Sangamo)-Destination vectors are available...assembly. Destination vectors, pcDNA-TAL-NC2 and pCAGGS-TAL-NC2, are mammalian expression/mRNA synthesis...

  7. CRISPR References and Information

    Type
    Collection
    ...efficiency. DeepSpCas9 (Link opens in a new window) : a webtool that provides models for SpCas9 gRNA design...pAc-sgRNA-Cas9 PDF 165.1 KB Marraffini Bacteria: pCas9 new spacer cloning pCas9 PDF 125.6 KB Marraffini Bacteria: pCRISPR...Musunuru CRISPRs in human pluripotent stem cells pCas9_GFP ; gRNA empty vector Link O'Connor-Giles Fly: gRNA... for Genomic Deletions in Mammalian Cell Lines pSpCas9(BB) (pX330) Link Parrott NEBuilder Assembly of ...

  8. Penn Vector Core Partnership with Addgene

    Type
    Collection
    ...ALL864 51503-AAV1 AAV pCAG-FLEX-tdTomato-WPRE Hongkui Zeng AV-9-ALL864 51503-AAV9 AAV pCAG-FLEX-tdTomato-WPRE... EYFP Karl Deisseroth AV-1-ALL854 51502-AAV1 AAV pCAG-FLEX-EGFP-WPRE Hongkui Zeng AV-1-PV0102 105531-AAV1... EYFP Karl Deisseroth AV-2-ALL854 51502-AAV2 AAV pCAG-FLEX-EGFP-WPRE Hongkui Zeng AV-2-PV0101 105530-AAV2... EYFP Karl Deisseroth AV-9-ALL854 51502-AAV9 AAV pCAG-FLEX-EGFP-WPRE Hongkui Zeng AV-9-PV0101 105530-AAV9...

  9. Lentiviral Prep Service

    Type
    Collection
    ...-targeting controls. This backbone contains SpCas9. SpCas9 and 76,441 unique sgRNAs targeting 19,114 human...non-targeting controls. This backbone does not contain SpCas9. 76,441 unique sgRNAs targeting 19,114 human genes...non-targeting controls. This backbone does not contain SpCas9. 78,637 unique sgRNAs targeting 19,674 mouse genes...

  10. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...to dimerization pcDNA3-CFP - Mammalian Expression pCAG-CFP - Mammalian Expression Cerulean 433 475 27 4.7... dimerization pcDNA3-EGFP - Mammalian Expression pCAG-GFP - Mammalian Expression pLV-eGFP - Mammalian ...530 68 5.9 Monomer pJL-mGold - Yeast Expression pCaggs-mGold - Mammalian Expression mCitrine 516 529 59... Mammalian Expression DsRed2 563 582 24 Tetramer pCAG-DsRed2 - Mammalian Expression DsRed2-N1 - Mammalian... - Mammalian Expression HcRed1 588 618 0.3 Dimer pCAG-HcRed - Mammalian Expression HcRed1-N1 - Mammalian...

  11. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...ataxia Bruce Tempel 30137 pCAX APP 695 APP CAG Alzheimer's Dennis Selkoe 30138 pCAX APP 751 APP CAG Alzheimer's...Dennis Selkoe 30143 pCAX APP delta CT APP CAG Alzheimer's Dennis Selkoe 30144 pCAX APP AENATA APP CAG ...Dennis Selkoe 30145 pCAX APP Swe/Ind APP CAG Alzheimer's Dennis Selkoe 30146 pCAX APP C99 APP CAG Alzheimer's...Dennis Selkoe 30147 pCAX APPs-695-alpha APP CAG Alzheimer's Dennis Selkoe 30149 pCAX APPs-751-alpha APP...-ko-sgRNA1-SpCas9 TARDBP U6 ALS Rajat Rohatgi 107858 pHBS953 TARDBP-Exon2-ko-sgRNA2-SpCas9 TARDBP U6 ALS...APP CAG Alzheimer's Dennis Selkoe 30154 pCAX FLAG APP APP Flag CAG Alzheimer's Dennis Selkoe 31255 pGEX...pScarlet-Kif5a KIF5A mScarlet CMV ALS Beverly Koller 118745 pCAG-Scarlet-Kif5a KIF5A mScarlet CAG ALS Beverly Koller...

  12. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...Cas9s: Three high fidelity Cas9 variants , SpCas9-HF, eSpCas9, and HypaCas9 display very low off-target...Researchers have expanded the CRISPR field beyond SpCas9, moving us closer to being able to target every... Staphylococcus aureus (SaCas9) is smaller than SpCas9 and more easily packaged in AAV. Cas9 orthologs...

  13. Church Lab CRISPR Plasmids

    Type
    Collection
    ...spectinomycin 48668 M-SPcas Mammalian S. pyogenes Cas9 expression, human optimized 48645 DS-SPcas Bacterial S. ...ACTTTAAAAGTATTCGCCAT), p15A/chloramphenicol 48657 DS-SPcasN- Bacterial nuclease-null SP Cas9 expression 48659...

  14. Chemogenetics Plasmids

    Type
    Collection
    ...Sternson 119739 pCAG PSAM4 GlyR IRES EGFP PSAM4-GlyR CAG IRES-EGFP No Sternson 119740 pCAG PSAM4-5HT3 HC...Psam4GlyR m3m4 HA PSAM4-GlyR m3m4 Syn No Sternson 123066 pCAG PSAM4 5HT3 LC IRES GFP PSAM4-5HT3 LC CAG IRES-EGFP...

  15. CRISPR Plasmids - Tagging

    Type
    Collection
    ... AAVS1 genomic safe harbor locus using untagged SpCas9 (Addgene 41815 or #44719 ) in combination with ...using an all in one vector from the Doyon lab, eSpCas9(1.1)_No_FLAG_AAVS1_T2 (Addgene #79888) , which ... integrating at the AAVS1 "safe harbor" locus: eSpCas9(1.1)_No_FLAG_AAVS1_T2 Doyon Lab TAP Tagging Protocol...

  16. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ... NLS YFP IX Nucleus NLS YFP Connie Cepko 158000 pCaggs-NLS-PAmCherry1-GSS-EGFP Nucleus NLS mGold Francois...FUmGW Membrane Palmitoylation GFP Connie Cepko 14757 pCAG-mGFP Membrane Palmitoylation sequence from GAP43...Actin Filaments LifeAct mCherry Robin Shaw 21948 pCAG-mGFP-Actin Actin Filaments beta-actin EGFP Ryohei...

  17. Retrovirus Plasmids

    Type
    Collection
    ...35617 pCAG-Eco Envelope Ecotropic MLV expressing envelope plasmid Nienhuis and Salmon 35616 pCAG-VSVG ...

  18. Lentivirus Plasmids

    Type
    Collection
    ...35617 pCAG-Eco N/A Envelope Ecotropic MLV expressing envelope plasmid Nienhuis and Salmon 35616 pCAG-VSVG...

  19. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...purposes. NOTE: Here we discuss the usage of the pSpCas9(BB) plasmid (pX330) (Addgene plasmid ID 42230)....allows for the simultaneous expression of sgRNA and SpCas9, but does not contain markers for selection 1 ....

  20. Immunology Research Plasmids and Resources

    Type
    Collection
    ... HSP86, HSP89A, HSP90A, HSP90N, HSPC1, HSPCA, HSPCAL1, HSPCAL4, HSPN, Hsp89, Hsp90, LAP2 HSP90AB1 heat...VIRG, VPAC1, VPCAP1R VIPR2 vasoactive intestinal peptide receptor 2 FLJ16511, VPAC2, VPCAP2R XCL1 chemokine...
Showing: 1 - 20 of 32 results