...EGFP/NLS Ple12 AVP pEMS1241 EGFP/NLS Ple12 AVP pEMS1482 intron-lacZ/NLS Ple13 AVP pEMS1242 EGFP/NLS Ple15...intron-lacZ/NLS Ple36 CRH pEMS1153 EGFP/NLS Ple37 CRH pEMS1154 EGFP/NLS Ple38 CRH pEMS1155 EGFP/NLS Ple39 CRH...cre/NLS Ple50 DBH pEMS1361 EGFP/NLS Ple51 DBH pEMS1113 EGFP/cre/NLS Ple51 DBH pEMS1362 EGFP/NLS Ple52 DCX...EGFP/NLS Ple53 DCX pEMS1199 EGFP/NLS Ple53 DCX pEMS1524 intron-lacZ/NLS Ple54 DCX pEMS1200 EGFP/NLS Ple55...intron-lacZ/NLS Ple56 DDC pEMS1125 EGFP/NLS Ple57 DDC pEMS1126 EGFP/NLS Ple58 DDC pEMS1127 EGFP/NLS Ple58 DDC...EGFP/NLS Ple63 DRD1 pEMS1168 EGFP/NLS Ple64 FEV pEMS1129 EGFP/cre/NLS Ple64 FEV pEMS1398 EGFP/NLS Ple66...EGFP/NLS Ple81 GAL pEMS1177 EGFP/NLS Ple84 GAL pEMS1180 EGFP/NLS Ple85 GCHFR pEMS1122 EGFP/cre/NLS Ple85...

...containing SFFV promoter, dCas9 that is fused to 2x NLS and tagBFP 46911 pHR-SFFV-dCas9-BFP-KRAB Human expression...containing SFFV promoter, dCas9 that is fused to 2x NLS, tagBFP and a KRAB domain 46912 pMSCV-LTR-dCas9-VP64...containing MSCV LTR promoter, dCas9 that is fused to 2x NLS, VP64 and tagBFP 51023 pSLQ1658-dCas9-EGFP Human ...expression vector containing dCas9 that is fused to 2x NLS and EGFP for CRISPR imaging 51024 pSLQ1651-sgTelomere...containing MSCV LTR promoter, dCas9 that is fused to 2x NLS, p65 activation domain and tagBFP 46914 pU6-sgGFP-NT1...CEN/ARS vector (Leu2) that contains dCas9 fused to NLS controlled by TDH3 promoter 46921 pTDH3-dCas9-Mxi1...

...8, 9, rg*, PHP.eB Fishell 99130 pAAV-mDlx-NLS-mRuby2 Dlx NLS-mRuby2 Constitutive 1, 2, 8, 9, rg*, PHP....Constitutive 2, 5, rg*, PHP.eB Gradinaru 104061 CAG-NLS-GFP CAG NLS-GFP Constitutive PHPeB Gradinaru 105530 pAAV.CMV.PI.EGFP.WPRE.bGH...pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP EF1a NLS-mCherry or nls-EGFP Cre dependent 1, 2, 5, 8, 9, rg* Harvey...Constitutive 1, 8 Deisseroth 135630 pAAV-S5E2-dTom-nlsdTom E2 regulatory dTomato Constitutive 1, 9, PHP.eB...oScarlet Constitutive 8 Deisseroth 192552 pAAV-CAG-2xNLS-tdTomato-WPRE-hGHpolyA CAG tdTomato Constitutive...

... 37354 pQC NLS mCherry IX Nucleus NLS mCherry Connie Cepko 37341 pQC NLS YFP IX Nucleus NLS YFP Connie...pCaggs-NLS-PAmCherry1-GSS-EGFP Nucleus NLS mGold Francois St-Pierre 14893 pEBFP2-Nuc Nucleus NLS EBFP2 ...Nuc Nucleus NLS mKalama1 Robert Campbell 73205 pcDNA3.1-NLS-myc-dL5-2xG4S-mCer3 Nucleus NLS (from Mak16p...98875 3xnls-mNeonGreen Nucleus 3xNLS mNeonGreen Dorus Gadella 98817 3xnls-mTurquoise2 Nucleus 3xNLS mTurquoise2...mTurquoise2 Dorus Gadella 98816 3xnls-mScarlet-I Nucleus 3xNLS mScarlet-I Dorus Gadella 98876 4xmts-mNeonGreen...

...Flp-dependent Control Deisseroth 99130 pAAV-mDlx-NLS-mRuby2 Dlx NLS-mRuby2 Control Gradinaru 83900 pAAV-mDlx-...Color-flipping switch. Expresses NLS-mCherry in the absence of Cre, and expresses NLS-EGFP in Cre-positive cells...Recombinases Janelia 51085 AAV-hSyn1-GCaMP6f-P2A-nls-dTomato Syn GCaMP6f and physically separate nuclear...Calcium sensor Ting 51083 AAV-EF1a-DIO-GCaMP6f-P2A-nls-dTomato EF1a GCaMP6f and physically separate nuclear...Calcium sensor Fishell 51084 AAV-hSyn1-GCaMP6s-P2A-nls-dTomato Syn GCaMP6s and physically separate nuclear...

...Gradinaru 104061-PHPeB CAG-NLS-GFP AAV expression of nuclear localized GFP with SV40 NLS Viviana Gradinaru 105448...of the tTA Viviana Gradinaru 99130-AAV1 pAAV-mDlx-NLS-mRuby2 An AAV genome that expresses nuclear localized... enhancer Viviana Gradinaru 99130-AAV2 pAAV-mDlx-NLS-mRuby2 An AAV genome that expresses nuclear localized... enhancer Viviana Gradinaru 99130-AAV8 pAAV-mDlx-NLS-mRuby2 An AAV genome that expresses nuclear localized... enhancer Viviana Gradinaru 99130-AAV9 pAAV-mDlx-NLS-mRuby2 An AAV genome that expresses nuclear localized...enhancer Viviana Gradinaru 99130-AAVrg pAAV-mDlx-NLS-mRuby2 An AAV genome that expresses nuclear localized...enhancer Viviana Gradinaru 99130-PHPeB pAAV-mDlx-NLS-mRuby2 An AAV genome that expresses nuclear localized...

...pAAV-hDlx-GiDREADD-dTomato-Fishell-5 hM4D(Gi) - Inhibition NLS-dTomato none 1, 9, rg* Fishell 83897 pAAV-hDlx-GqDREADD-dTomato-Fishell...pAAV-hDlx-GqDREADD-dTomato-Fishell-4 hM3D(Gq) - Activation NLS-dTomato none 1, 9, rg* Fishell 50472 pAAV-GFAP-HA-rM3D...

...pAAV-CAG-eYFP CAG EYFP Control Gradinaru 104061 CAG-NLS-GFP CAG NLS-GFP Control Gradinaru 105530 pAAV.CMV.PI.EGFP.WPRE.bGH...pAAV-mDlx-GFP-Fishell-1 Dlx GFP Control Fishell 99130 pAAV-mDlx-NLS-mRuby2 Dlx mRuby2 Control Gradinaru 104055 pAAV-CAG-eYFP...dTomato/nlsdTomato Control Fishell 213936 pAAV_BiPVe4_dTomato_nlsdTomato BiPVe4 dTomato/nlsdTomato Control...Control AIBS , Ting 213814 pAAV_BiSSTe10_dTomato_nlsdTomato BiSSTe10 dTomato/nlsdTomato Control Fishell 213829...213829 pAAV_BiCHATe27_dTomato_nlsdTomato BiCHATe27 dTomato/nlsdTomato Control Fishell 213855 pAAV_BiVIPe4...BiVIPe4 dTomato/nlsdTomato Control Fishell 213914 pAAV_BiLAMP5e3_dTomato_nlsdTomato_nlsdTomato BiLAMP5e3 dTomato...Control Fishell 213940 pAAV_BiPVe3_dTomato_nlsdTomato BiPVe3 dTomato/nlsdTomato Control Fishell 213944 pAAV_BiSSTe4...

...AAV-EF1a-DIO-GCaMP6f-P2A-nls-dTomato EF1a GCaMP6f dTomato Cre dependent rg* Ting 51085 AAV-hSyn1-GCaMP6f-P2A-nls-dTomato..., Huebener , Rose 51082 AAV-EF1a-DIO-GCaMP6s-P2A-nls-dTomato EF1a GCaMP6s dTomato Cre dependent 1 Ting...Cre dependent 5 Larsen 51084 AAV-hSyn1-GCaMP6s-P2A-nls-dTomato Syn GCaMP6s dTomato Constitutive 1, rg* Ting...

...IRF-1 promoter. 98041 Sa-dCas9-NLS-3xFLAG/pcDNA3.1 Expresses Sa-dCas9-NLS-3xFLAG in mammalian cells for...

...and 47389 include a Flag epitope tag and an SV40 NLS at the N terminus, a 152-residue deletion from the...acids after the repeat domain, and C-terminal SV40 NLS and HA tags. Plasmid 47389 also contains the VP64...

...cloning in gRNA: pSimpleII-U6-tracr-U6-BsmBI-NLS-NmCas9-HA-NLS(s) PDF 47.5 KB Vosshall and Matthews CRISPR...

...permits programmable protein phosphorylation. Pirman NL, Barber KW, Ma NJ, Haimovich AD, Rogulina S, Isaacs...-Free Protein Synthesis. Oza JP, Aerni HR, Pirman NL, Rogulina S, ter Haar CM, Isaacs FJ, Rinehart J, ...

...share: In vivo data: Retrograde hSyn1-GCaMP6f-P2A-nls-dTomato Detailed protocol: AAV Production in HEK293...

... promoter pAdx-CMV-YFP 73348 Express YFP with an NLS from the CMV promoter pAdx-CMV-dsRed 73349 Expresses...

...firefly luciferase Javier Alcudia 113442 pcDNA3.1 NL NanoLuc® CMV Mammalian expression of Nanoluc with...expression of firefly luciferase Georg Weber 113450 pLenti NL NanoLuc® Ubc Lentiviral expression of Nanoluc with...

...dCAS9-VP64 with 2A Blast resistance marker (EF1a-NLS-dCas9(N863)-VP64-2A-Blast-WPRE) Zhang 61426 lenti...

...(red flour beetle) Averof 119868 pGEM-NLS-Cre-2A-mCherry-FKF NLS-Cre, mCherry T7 Bacterial Sauka-Spengler...miRNA expression PGK Lentiviral Jacks 17736 pOG231 NLS-Cre CMV Mammalian Wahl 19131 pIC-Cre Cre Bacterial...pLOX-CW-CRE Cre CMV Lentiviral Trono 12265 pHR-CMV-nlsCRE Cre CMV Lentiviral Trono 12493 p259 pCMV-CRE-M ...Fsyn_Cre Cre hSyn Lentiviral Cohen 111150 Prrx1:TFPnls-T2a-Cre-Ert TFP and Cre-ERT2 - Tamoxifen inducible...tissue mouse Prrx1 Vertebrate Tanaka 111151 Col1A2:TFPnls-T2a-mErt-Cre-Ert TFP and Cre-ERT2 - Tamoxifen inducible...fibroblasts mouse Col1A2 Vertebrate Tanaka 111152 Col2a1:TFPnls-mErt-Cre-Ert TFP and Cre-ERT2 - Tamoxifen inducible...

...packaging construct encoding Tat. Reiser 17531 pCD/NL-BH*DDD 2nd Packaging Contains Gag/Pol, Tat, Rev. ...

... See full Fluorescent Protein Guide NLS Nuclear Localization pENTR4-myc-nuc - pEntry ...