... Gadella 85053 pmScarlet-i_H2A_C1 Nucleus H2A mScarlet-I Dorus Gadella 85052 pmScarlet-H_H2A_C1 Nucleus...Nucleus H2A mScarlet-H Dorus Gadella 85051 pmScarlet_H2A_C1 Nucleus H2A mScarlet Dorus Gadella 18982 pHIV-...Bruchez 85065 pmScarlet-I_peroxisome_C1 Peroxisome SRL mScarlet-I Dorus Gadella 85064 pmScarlet-H_peroxisome_C1...Peroxisome SRL mScarlet-H Dorus Gadella 85063 pmScarlet_peroxisome_C1 Peroxisome SRL mScarlet Dorus Gadella...85045 pmScarlet_alphaTubulin_C1 Microtubules alpha-tubulin mScarlet Dorus Gadella 85046 pmScarlet-H_alphaTubulin_C1...alpha-tubulin mScarlet-H Dorus Gadella 85047 pmScarlet-i_alphaTubulin_C1 Microtubules alpha-tubulin mScarlet-I Dorus...85054 pLifeAct_mScarlet_N1 Actin Filaments LifeAct mScarlet Dorus Gadella 85055 pLifeAct_mScarlet-H_N1 ...

...EYFP-WPRE-HGHpA Karl Deisseroth AV-1-20938M 100054-AAV1 pAAV.CAG.hChR2(H134R)-mCherry.WPRE.SV40 Karl Deisseroth...-EYFP Karl Deisseroth AV-1-26967M 100049-AAV1 pAAV.hSynap.ChETA(E123T/H134R)-eYFP.WPRE.hGH Karl Deisseroth...pAAV-Ef1a-DIO ChETA-EYFP Karl Deisseroth AV-1-26969P 26969-AAV1 pAAV-CaMKIIa-hChR2(H134R)-EYFP Karl Deisseroth AV...pAAV-CaMKIIa-eNpHR 3.0-EYFP Karl Deisseroth AV-1-26973P 26973-AAV1 pAAV-hSyn-hChR2(H134R)-EYFP Karl Deisseroth AV-1...H134R)-mCherry Karl Deisseroth AV-1-35503 35503-AAV1 pAAV-Ef1a-DIO hChR2(C128S/D156A)-EYFP Karl Deisseroth...EYFP-WPRE-HGHpA Karl Deisseroth AV-5-20938M 100054-AAV5 pAAV.CAG.hChR2(H134R)-mCherry.WPRE.SV40 Karl Deisseroth...pAAV-Ef1a-DIO eNpHR 3.0-EYFP Karl Deisseroth AV-5-26968P 26968-AAV5 pAAV-Ef1a-DIO ChETA-EYFP Karl Deisseroth AV-5-...

...124650 pAAV-CamKIIa-C1V1(t/t)-mScarlet-KV2.1 CaMKII C1V1 (t/t) mScarlet Constitutive 9 Harvey 59170 pAAV-Syn-Chronos-GFP...pAAV-CamKIIa-ChrimsonR-mScarlet-KV2.1 CaMKII ChrimsonR (soma-targeted) mScarlet Constitutive 9 Harvey ...pAAV-CaMKIIa-ChRmine-mScarlet-Kv2.1-WPRE CamKII ChRmine (high-photocurrent, red-shifted) mScarlet Constitutive...-hSyn-ChRmine-mScarlet-Kv2.1-WPRE Syn ChRmine (high-photocurrent, red-shifted) mScarlet Constitutive 8...pAAV-Ef1a-DIO-ChRmine-mScarlet-WPRE EF1a ChRmine (high-photocurrent, red-shifted) mScarlet Cre dependent 5 ...137158 pAAV-nEF-ChRmine-mScarlet nEF ChRmine (high-photocurrent, red-shifted) mScarlet Constitutive 8 Deisseroth...pAAV-nEF-Con/Fon-ChRmine-oScarlet nEF ChRmine (high-photocurrent, red-shifted) oScarlet Cre and Flp dependent...

... Gateway GTPase Arl4c 10123 201/192 Gateway GTPase Arl4d 379 201 Gateway GTPase Arl5a 26225 179 Bacterial...Gateway GTPase Arl5b 221079 179 Bacterial (pET), Gateway GTPase Arl5c 390790 179 GTPase Arl6 84100 186 Bacterial...pET), Gateway GTPase Arl9 132946 187 GTPase Arl10 285598 276; 244 GTPase Arl11 115761 196 Bacterial (pET...Gateway GTPase Arl13a 392509 256 GTPase Arl13b 200894 428 Mammalian (pcDNA3), Gateway GTPase Arl14 80117 192... (pcDNA3), Gateway GTPase Arl1 400 181 Bacterial (pET), Gateway GTPase Arl2 402 184 Bacterial (pET), Mammalian... Gateway GTPase Arl3 403 182 Bacterial (pET), Mammalian (pcDNA3), Gateway GTPase Arl4a 10124 200 Bacterial...Bacterial (pET), Gateway GTPase Arl8a 127829 186 Bacterial (pET), Gateway GTPase Arl8b 55207 186 Bacterial (pET...

...pAAV-CaMKIIa-mScarlet CamKIIa mScarlet Constitutive 8 Deisseroth 131001 pAAV-hSyn-mScarlet Syn mScarlet Constitutive...Foff 2.0-oScarlet EF1a oScarlet Cre dependent 8 Deisseroth 137138 pAAV-Ef1a-Coff/Fon-oScarlet EF1a oScarlet..., PHP.eB Dimidschstein 137135 pAAV-Ef1a-oScarlet EF1a oScarlet Constitutive 8 Deisseroth 192552 pAAV-CAG..., 8, 9, rg* Harvey 131002 pAAV-Ef1a-DIO mScarlet EF1a mScarlet Cre-dependent 1 Deisseroth 50457 pAAV-hSyn-DIO-EGFP...dependent 8 Deisseroth 137136 pAAV-Ef1a-Con/Fon-oScarlet EF1a oScarlet Cre and Flp dependent 8 Deisseroth 137137...

...AND NOT Cre 137135 pAAV-Ef1a-oScarlet None 137136 pAAV-Ef1a-Con/Fon-oScarlet Cre AND Flp 137137 pAAV-Ef1a-Con...pAAV-Ef1a-Con/Foff 2.0-oScarlet Cre AND NOT Flp FRT/F5 137138 pAAV-Ef1a-Coff/Fon-oScarlet Flp AND NOT Cre Dual...137158 pAAV-nEF-ChRmine-mScarlet None 137159 pAAV-nEF-Con/Fon-ChRmine-oScarlet Cre AND Flp 137160 pAAV-nEF-Coff...Fon-ChRmine-oScarlet Cre AND NOT Flp FRT/F5 137161 pAAV-nEF-Con/Foff 2.0-ChRmine-oScarlet Flp AND NOT ..., Awatramani R. 2018. Mapping projections of molecularly defined dopamine neuron subtypes using intersectional...LE, Ramakrishnan C, Silberberg G, Deisseroth K, Carlén M, Meletis K. 2019. A hypothalamus-habenula circuit...

...Expression mScarlet-I3 568 592 68 4.2 2 min Monomer pmScarlet-I3_C1 - Mammalian Expression mScarlet3 569 592...Monomer pmScarlet3_C1 - Mammalian Expression mScarlet 569 594 71 5.3 2.9 hr Monomer pmScarlet_C1 - Mammalian...Maturation Structure Plasmids mScarlet-H 551 592 15 4.8 4.4 hr Monomer pmScarlet-H_C1 - Mammalian Expression...Mammalian Expression pCS2+mScarlet-C Cloning Vector - Mammalian Expression mScarlet-I 569 593 57 5.4 36 min...min Monomer pmScarlet-i_C1 - Mammalian Expression mStrawberry 574 596 26 4.5 50 min Monomer mStrawberry-N1...

...118744 pScarlet-Kif5a KIF5A mScarlet CMV ALS Beverly Koller 118745 pCAG-Scarlet-Kif5a KIF5A mScarlet CAG ...Parkinsonism, early-onset Roger Reeves 53822 SYNJ1-170_pCSdest SYNJ1 CMV, SP6 Parkinsonism, early-onset Roger...telangiectasia Charles Swanton 173580 Lenti-sgAtm#2/Cre ATM CMV Ataxia telangiectasia Charles Swanton 173759...Arrowsmith 184825 pSN837 KIF5A mScarlet CMV ALS Kyoko Chiba 184826 pSN838 KIF5A mScarlet CMV ALS Kyoko Chiba 184827...184827 pSN526 KIF5A mScarlet Strep polH ALS Kyoko Chiba 184832 pSN840 KIF5A mScarlet Strep polH ALS Kyoko...FLAG-Synaptojanin 1-145 SYNJ1 Flag CMV Parkinsonism, early-onset Pietro De Camilli 22292 pcDNA3-FLAG-Synaptojanin...FLAG-Synaptojanin 1-170 SYNJ1 Flag CMV Parkinsonism, early-onset Pietro De Camilli 22293 pEGFPC1-FLAG-Synaptojanin...

...experimental systems. LumiLuc and LumiScarlet : LumiLuc and LumiScarlet are ATP-independent luciferases ...be lysed or the substrate must be delivered intracellularly in order to measure luminescence. Gaussia luciferase... (Rluc8) through an Ala-Glu-Leu linker to the circularly permuted Venus fluorophore. Learn more about ...Insertion of imperfect miRNA sequence in the 3' UTR Carl Novina 33154 pAC-Luc Firefly Creation of N-terminal...fusion for expression in plants, co-expressed with mScarlet-I for ratiometric imaging David Nelson 141301 ...

...evolved adaptive immune defenses termed clustered regularly interspaced short palindromic repeats (CRISPR)...Cas9. Mali P, Yang L, Esvelt KM, Aach J, Guell M, Dicarlo JE, Norville JE, Church GM. Science . 2013 Jan ...Saccharomyces cerevisiae using CRISPR-Cas systems. Dicarlo JE, Norville JE, Mali P, Rios X, Aach J, Church...activity, while particular protospacers interact nonlinearly with different PAM sequences to determine overall...favorable protospacers and applications requiring particularly tight binding. In these cases it may be advisable...

...6R] Mad3 Human Mouse IgG2a 140076 Anti-Arl13b [N295B/66R] Arl13b? Mouse Mouse IgG2a 140077 Anti-ZNF423 ...TrpV3 Rat Mouse IgG2a 188211 Anti-BBS3/Arl6 [N302/10R] BBS3/Arl6 Human Mouse IgG2a 188212 Anti-PSD-95 [... channel Rat Mouse IgG2b 199413 Anti-Arl13b [N295B/66R-2b] Arl13b Rat Mouse IgG2b 199414 Anti-GluN1/NR1...41R] EAAC1 Rat Mouse IgG2a 206616 Anti-Arl13b [N295B/54R] Arl13b Mouse Mouse IgG2a 206618 Anti-GluN3A/NR3A...1] VGlut2 Rat Mouse IgG1 206708 Anti-Arl13b [N295B/66R-1] Arl13b Mouse Mouse IgG1 206709 Anti-NR1 glutamate... N29/29 VGlut2 Rat Mouse 182086 Arl13b scFv [N295B/66] N295B/66 Arl13b Mouse Mouse 182088 GABA(A)R, gamma2...

...language abilities. The disease can be classified as early-onset, where symptoms appear between a person’s ...appear during or after a person's mid-sixties. The early-onset form accounts for less than 10 percent of ...of all cases of Alzheimer's disease. Of these early-onset forms, a fraction of these are due to a mutation...neuron-specific reporter and a PAX7 inducible vector . Harley et al. Cell Rep. 2023 Dec 26. Induce CNS cells ...

... a neurodevelopmental disorder that presents in early toddlerhood primarily affecting females at an incidence...criteria, defined as the presence of relatively normal early development, regression with a loss of spoken language... strong predictor of symptom severity. Broadly, early gene disruptions, missense mutations located in ...window) PMID: 6638958 Kankirawatana et al. 2006. Early progressive encephalopathy in boys and MECP2 mutations...

...activity, leading to the development of tumors in early adulthood, a disorder known as Li-Fraumeni syndrome...Germline TP53 mutations and Li-Fraumeni syndrome. Varley JM. Hum Mutat. 2003 Mar;21(3):313-20. PubMed PMID...PMID: 12619118 . p53 mutant mice that display early ageing-associated phenotypes. Tyner SD, Venkatachalam...

...immunogenic properties of the final product, particularly in large animal studies. Endotoxin contamination...detected. pAAV-Ef1a-mCherry-IRES-Cre was a gift from Karl Deisseroth (Addgene viral prep # 55632-AAVrg). ...being discarded and replaced with a new vial of early passage cells. Approximately 2 weeks post-thaw, ...

...pAAV_EF1a-DIO-PdCO-mScarlet-ER-miniWPRE Expresses optimized PdCO in frame with mScarlet under control of...

...Publications CRISPR Overview Class 2 C lustered R egularly I nterspaced S hort P alindromic R epeat (CRISPR...strand separation and cutting at off-target sites. Similarly, SpCas9-HF1 lowers off-target editing through ...target DNA based on the gRNA targeting sequence. Early experiments demonstrated that targeting dCas9 to...genetic screening. Forward genetic screens are particularly useful for studying diseases or phenotypes for...Cas12a (also known as Cpf1) CRISPR C lustered R egularly I nterspaced S hort P alindromic R epeat, a bacterial...2013. Mali P, Yang L, Esvelt KM, Aach J, Guell M, DiCarlo JE, Norville JE, Church GM. Science . 339(6121)...

...Delivery AAV - Cas9 Mouse CRISPR ( C lustered R egularly I nterspaced S hort P alindromic R epeats) is ...oligos (design is indicated below) can be cloned scarlessly into the vector before the sgRNA scaffold. The..., and a pair of annealed oligos can be cloned scarlessly into the vector before the sgRNA scaffold. The...

...transcription factors, and reporter genes. pCS-PesaRlux 47640 PesaR 3OC6HSL (Quorum sensing molecule) ...The EsaR-responsive promoter can be found in pCS-PesaRlux controlling luciferase expression. pCW-LIC 26098...performing a bacterial two-hybrid (B2H) screen of modularly assembled zinc fingers. Return to Top Do you have...

...the construct leads to a change in signal of a circularly permuted fluorescent protein (cpFP) or Förster...Neuron. 2020 Jun 20. pii: S0896-6273(20)30432-3. Karl Deisseroth , INTRSECT 2.0 Project Calcium Expression...Neuron. 2020 Jun 20. pii: S0896-6273(20)30432-3. Karl Deisseroth , INTRSECT 2.0 Project Calcium Mitochondrial... voltage sensor to detect neural activity (Ace-mScarlet) A high-speed, bright, red fluorescent voltage...