...Foundation Search Use Addgene's Search to find plasmids available to industry scientists while also adding additional...growing collection of plasmids that are available to industry. Learn more about the available plasmids and ... Distribution to Industry Featured Collections Search Plasmids Browse Plasmids Browse...of the plasmids that are currently available to industry scientists. ID Plasmid Gene/Insert PI Kits Kit...us know what you would like to see available to industry scientists. Please note this does not guarantee...access to plasmid research tools. Pricing will be indicated on the product pages after you login to your account... information on pricing, payment, and billing. Industry Material Transfer Agreements Most Addgene orders...

...Collections Tetracycline (Tet) Inducible Expression Tetracycline (Tet) Inducible Expression Additional Resources...of tetracycline, tetracycline binds to tetR. This precludes tTA binding to the tetO sequences and subsequent...system induction. Investigate whether or not your serum is tetracycline-free. Tetracycline-inducible Empty...Empty Backbones Find a construct that will allow you to insert and induce your gene of interest. ID Plasmid...absence of binding of tTA or rtTA None On Vogelstein 25735 pSLIK-Neo Lentiviral vector for inducible expression...vectors for tet-inducible gene or miRNA expression rtTA On Alexeyev 14901 pCL-CTIG The inducible cassette contains...Overview of tetracycline regulated inducible transcriptional activation or repression Plasmid...

... TALengineering Reagents TALE Repeat Plasmids Individual REAL TALE Repeat Plasmids You may also like.....longer arrays. These 28 plasmids are available individually or as a set as part of the Joung Lab REAL Assembly...

...: the N - t erminal D omain (NTD) the M ethyl B inding D omain (MBD) a T ranscriptional R epressor D omain...iPSC (Link opens in a new window) PMID: 26944080 (Link opens in a new window) Alysson Muotri Q83X C247T...iPSC (Link opens in a new window) PMID: 26944080 (Link opens in a new window) Alysson Muotri c.806delG...a new window) Wendy Gold A140V C419T A140V M Fibroblasts Unpublished (Link opens in a new window) Wendy...a new window) Wendy Gold HO_AN G917A R306H F Fibroblasts Unpublished (Link opens in a new window) Wendy... opens in a new window) RettBase - MECP2 mutation database (Link opens in a new window) OMIM Rett Syndrome...in a new window) OMIM MECP2 - MECP2 curated literature information (Link opens in a new window) UniProt...

...fluorescent cAMP indicator (Flamindo2) Genetically-encoded yellow fluorescent cAMP indicator with an expanded...protein-based cAMP indicator (Pink Flamindo) Red fluorescent protein-based cAMP indicator applicable to optogenetics...Calcium Indicators (Constitutive or Cre-dependent) jGCaMP8 Fast Genetically Encoded Calcium Indicators. Janelia...encoded Calcium indicators (GECOs) An Expanded Palette of Genetically Encoded Ca2+ Indicators. Science. 2011...calcium indicator for fluorescence using HaloTag ligands A modular chemigenetic calcium indicator enables...fluorescence indicator of K+ for optical imaging (GINKO) Genetically encoded fluorescent indicators for imaging...Multicolor palette of ATP indicators (MaLion) RGB-color intensiometric indicators visualize spatiotemporal...

...structure are responsible for binding the antigen, and are called the antigen binding fragments (Fab). The tips...recognizing and binding to epitopes on antigens, they are also capable of recognizing and binding epitopes on...single individual, giving a high probability that an antibody will be able to recognize and bind to any... and binds to the protein of interest. Signal strength is determined by how many antibodies bind the protein...detection antibody is added and binds to the bound protein. If using the indirect sandwich method, a conjugated...? Will you be using a direct or indirect detection method? If indirect, do you already have secondary ...tools used in the lab to specifically and tightly bind to other proteins. Antibodies, produced as part ...

...Anhydrotetracycline inducible expression of wild-type Cas9 from S. pyogenes for inducing double stranded ...coat protein binding sites Philippe Cluzel RNA localization: Contains a 2x array of the binding sequence ... the MS2 binding sites within this plasmid. pZE31-DsRed-ms2x2 26248 MS2 coat protein binding sites Philippe...localization: Contains a 2x array of the binding sequence for RNA-binding protein MS2 fused to dsRED. Can be... Tac (lactose/IPTG inducible) MBP, 6x His David Waugh Empty lactose/IPTG inducible vector for expression.../IPTG inducible) C-terminal TEV cleavable 6x His Cheryl Arrowsmith Empty lactose/IPTG inducible vector...expression upon induction) lactose/IPTG Cheryl Arrowsmith Empty lactose/IPTG inducible vector. pPro Series...

... tet inducible Cre-GFP fusion tet-hCMV Mammalian Sauer 11961 pBS596 tet-hCMV-GFPcre tet inducible Cre-...Tamoxifen inducible CAG Mammalian Cepko 15028 Hsp68-CreER(TM) (DM#264) CRE-ER(TM) - Tamoxifen inducible Hsp68...Tamoxifen inducible; Targeting vector for Pax7 Tanaka 111187 pBADZ-HisCre Cre; arabinose inducible. PBAD ...Tamoxifen inducible CAG Mammalian Capecchi 125749 pCAG-sfGFP-iCre-ERT2 iCre-ERT2 - Tamoxifen inducible CAG ...and recombination. Use Addgene's curated tables to find Cre-lox plasmids for your experiments. Plasmid...constructs also contain fluorescent labels that indicate if recombination has occurred, allowing for direct...expression and to optimize Cre expression once it is induced. Addgene has a wide variety of Cre recombinase ...

...Acinar Cells induced Beta Cells Adenoviral Mouse Long-term persistence and development of induced pancreatic... Shinya Yamanaka’s breakthrough development of induced pluripotent stem cells (iPSCs) in both mouse and...expression of key factors. The tables below will help you find plasmids available from Addgene that can be used...search the iPSC Neurodegenerative Disease Initiative (iNDI) Collection for plasmids that can be used to create...Browse the table below for plasmids used to create induced pluripotent stem cells (iPSCs). Sort the table ...species, or PI and then select the article link to find more information. Delivery Method Species Description...and Lin28 from four separate lentiviral plasmids Induced Pluripotent Stem Cell Lines Derived from Human ...

...a new window) Brain Banks (Link opens in a new window) Rodent Models (Link opens in a new window) Michael...reporter and a PAX7 inducible vector . Harley et al. Cell Rep. 2023 Dec 26. Induce CNS cells with concomitant...Foundation (Link opens in a new window) A foundation dedicated to finding a cure for Parkinson's disease... opens in a new window) Research workspace on Protocols.io (Link opens in a new window) . This resource...Find out about the plasmid collections available at Addgene that may be useful for neurodegeneration ... Addgene has assembled the following to help you find information and plasmid collections that may help...triplet series within the huntingtin (HTT) gene. An individual with the mutated HTT gene will have huntingtin...

.... gRNA binding induces a conformational change in Cas9, shifting it into an active, DNA-binding configuration...( C lustered R egularly I nterspaced S hort P alindromic R epeat) technologies. Before CRISPR, genome ...composed of a scaffold sequence necessary for Cas-binding and a user-defined ∼20-nucleotide spacer that defines... otif (PAM) The PAM sequence (NGG) serves as a binding signal for Cas9, but the exact sequence depends...with the target DNA. Once the Cas9-gRNA complex binds a putative DNA target, the seed sequence (8–10 bases...undergoes a second conformational change upon target binding. In this formation, its nuclease domains, RuvC ...causes small nucleotide insertions or deletions (indels) at the DSB site. Therefore, a population of cells...

...available to industry, in addition to academics and nonprofits, and this is denoted in the Industry column ...Plasmid Distribution to Industry page. The list of plasmids available to industry is continually growing... SARS-CoV-2. Find plasmids related to these genes below. ID Plasmid Description Industry PI For more information...protocols and design resources (Link opens in a new window) Find Zhang Lab Cas13a plasmids Additional protocol...diagnostics: SARS-CoV-2 DETECTR (Link opens in a new window) Find Doudna Lab Cas12a plasmids Addgene's full CRISPR...Link opens in a new window) BST-LF expression plasmid from Andrew Ellington's lab. Find plasmid tools that...like that of SARS-CoV and MERS CoV, depends on binding of the viral spike (S) protein to cellular receptors...

... increase its stability and DNA binding activity, and allow it to bind transcriptional co-activators and...not indicate a specific relationship. p53 Pathway Color is used for clarity and does not indicate a specific...and DNA-damage-inducible; alpha, beta, or gamma IGF-BP3 Insulin-like growth factor binding protein 3 KAI...protein p53 regulated apoptosis inducing protein 1 p53R2 p53 inducible, ribonucleotide reductase M2 B ...apoptosis effector PIDD p53-induced death domain protein 1 PIGs Etoposide induced 2.4 PIRH-2 Ring finger and...these mutations are missense mutations in the DNA-binding domain, often at one of six hotspot residues. These... cancer. p53 Pathway Plasmids Click on a name to find available plasmids for the gene, or browse the gene...

...Find intersectional expression plasmids that combine multiplexed recombinases, including Cre, Flp, and...Standard Operating Procedure (Link opens in a new window) with general principles for working with INTRSECT...experimental design. How-to guide (Link opens in a new window) for the molecular design and testing of novel ...plasmids. Online spreadsheet (Link opens in a new window) of Flp-recombinase-expressing transgenic mouse...inhibitory opsins , genetically-encoded calcium indicators, and rabies targeting genes. Recombinases Addgene...Methods 11(7):763-72. PubMed (Link opens in a new window) Chuhma N, Mingote S, Yetnikoff L, Kalmbach A, ...Elife. 7. pii: e39786. PubMed (Link opens in a new window) Cummings KA, Clem RL. 2020. Prefrontal somatostatin...

...Link opens in a new window) . GenET (Genome Editing Toolkit) (Link opens in a new window) is a library of...endonuclease) Tools: Cas-OFFinder & Cas-Designer From the Kim Lab , Cas-OFFinder identifies gRNA target ...of California, Berkeley. CRISPR Software Sanger Indel Analysis ICE (Inference of CRISPR Edits) This new... datasets and allows the direct comparison of individual experiments. PubMed PMID 27404874 . MAGeCK Model-based...their efficiency. DeepSpCas9 (Link opens in a new window) : a webtool that provides models for SpCas9 gRNA...gRNA design. DeepBE (Link opens in a new window) : a webtool that provides models for base editor (BE)...BE) gRNA design. DeepPrime (Link opens in a new window) : a webtool that provides models for prime editor...

...the ligand-binding pocket Bind specific subunit pairs, outside the ligand-binding pocket Bind specific ...antibodies that uniquely: Bind to the alpha subunit, outside the ligand-binding pocket Bind to the beta subunit... opens in a new window) based on yeast display technology (Link opens in a new window) and an optimized...Find unique recombinant antibodies targeting families of cell surface and secreted proteins from a collaboration...Protein Innovation (IPI) Collection! Here, you’ll find unique recombinant antibodies targeting families...model. Learn more about IPI (Link opens in a new window) Glypican Antibodies Glypicans are a family of ...specific subunit pairs, blocking ligand binding and receptor function Figure courtesy of IPI Browse Integrin ...

...ULBP1 UL16 binding protein 1 RAET1I ULBP2 UL16 binding protein 2 N2DL2, RAET1H ULBP3 UL16 binding protein...kinase binding protein DAP12, KARAP, PLOSL ULBP1 UL16 binding protein 1 RAET1I ULBP2 UL16 binding protein...RING11 TAPBP TAP binding protein (tapasin) NGS17, TAPA, TPN, TPSN, tapasin TAPBPL TAP binding protein-like...factor beta binding protein 1 MGC163161 LTBP2 latent transforming growth factor beta binding protein 2 ... RAB GTPase binding effector protein 1 RAB5EP, RABPT5 RABEP2 rabaptin, RAB GTPase binding effector protein...cell is primed to recognize a specific antigen, it finds and attacks pathogens or infected cells expressing...T cell, whereas T cell tolerance or anergy are induced when the TCR is engaged without co-stimulation ...

...CAG Alzheimer's Dennis Selkoe 30145 pCAX APP Swe/Ind APP CAG Alzheimer's Dennis Selkoe 30146 pCAX APP ...Aleksandra Radenovic 114194 pc DNA FRT TO- APPSwed/Ind APP CMV Alzheimer's Aleksandra Radenovic 114410 AICSDP...Huntington's Susan Lindquist 1178 p416 46Q GPD HTT GFP GPD Huntington's Susan Lindquist 1179 p416 72Q GPD...Huntington's Susan Lindquist 1180 p416 103Q GPD HTT GFP GPD Huntington's Susan Lindquist 1181 p426 25Q GPD...Huntington's Susan Lindquist 1182 p426 46Q GPD HTT GFP GPD Huntington's Susan Lindquist 1183 p426 72Q GPD...Huntington's Susan Lindquist 1184 p426 103Q GPD HTT GFP GPD Huntington's Susan Lindquist 1185 p416 25Q GAL...Huntington's Susan Lindquist 1186 p416 103Q GAL HTT GFP GAL1 Huntington's Susan Lindquist 1187 p426 25Q GAL...

... in a new window) The Malate Dehydrogenase CUREs Community (MCC) (Link opens in a new window) develops...mentor future MCC members. Find a regional hub (Link opens in a new window) and contact the hub director...opens in a new window) — Learn how to get started with a CURE, explore MDH projects, find teaching resources...expression in bacteria and cloned into an IPTG-inducible His-tag expression vector. All His-tags are cloned... cloned at the C-terminus of MDH and, if indicated on the plasmid map, a TEV cleavage site is present ...plasmid page. Use the search bar or sort buttons to find plasmids based on: Organism or species (e.g., human.... DOI: 10.24918/cs.2021.43 (Link opens in a new window) External Collaboration Results in Student Learning...

...separate the individual crRNAs. (3) The tracrRNA and Cas9 nuclease form a complex with each individual, unique...directly after where the crRNA would bind. (5) After the complex binds, Cas9 separates the double stranded...CRISPR (Clustered Regularly Interspaced Short Palindromic Repeat) sequences were initially discovered in...protospacer adjacent motif (PAM). The CRISPR complex binds to the foreign DNA and cleaves it to destroy the...foreign DNA. Protospacers are separated by short palindromic repeat sequences. (1) The CRISPR array is transcribed...pre-crRNA). (2) The pre-crRNA is processed into individual crRNAs by a special trans-activating crRNA (tracrRNA...(tracrRNA) with homology to the short palindromic repeat. The tracrRNA helps recruit RNAse III and Cas9...