...double-strand break (DSBs) at a specific location based on a co-expressed gRNA-defined target sequence. DSBs are ...etc.) On the other hand, gRNA plasmids that do not co-express a Cas protein can be paired with a wide variety...Plasmid Promoter Cloning Enzyme(s) Delivery Resistance Co-expressed Cas9 Depositing lab Cas9 species = S. pyogenes...

...genome loci, the most efficient and easiest way is to co-transfect several plasmid together, with each plasmid...clone two spacers for each locus into PX330, then co-transfect or delivery the two plasmids together into...use double nickase, you need to have four spacers co-expressed and using PX335 to express the Cas9n (nickase..., test run them and then select the best guide to co-transfect in HR donor. Alternatively, a new system... 10uM oligo stock for each well on 24-well plate, co-deliver into the cells with the Cas9/sgRNAs vector...screened? Before starting your experiment, consider co-transfecting with GFP. This allows you to sort for...puromycin resistance cassette, such as PX459 ). After you co-transfect the CRISPR/Cas system with your homologous...

...field and every attendee of the meeting will be a co-author of the guide. We hope that this guide will...02139 Organizing Committee Chair: Dr. Melina Fan, Co-Founder and CSO, Addgene Organizing Committee: Dr...your participation in this meeting, you will be a co-author on this communication and you will be helping...

...etc.) On the other hand, gRNA plasmids that do not co-express a Cas protein can be paired with a wide variety...Plasmid Promoter Cloning Enzyme(s) Delivery Resistance Co-expressed Cas9 Depositing lab Cas9 species = S. pyogenes...

...basepair complementary region to any gene of interest. Co-expression of dCas9 and sgRNA can efficiently block...designed to target arbitrary sequences in the genome. Co-expression of both plasmids in bacteria could cause...fluorescent gating of transiently transfected cells. Co-expression of both plasmids in HEK293 cells could...

...etc.) On the other hand, gRNA plasmids that do not co-express a Cas protein can be paired with a wide variety...Plasmid Promoter Cloning Enzyme(s) Delivery Resistance Co-expressed Cas9 Depositing lab Cas9 species = S. pyogenes...

...method for the systematic and quantitative analysis of co-immunoprecipitations using both Renilla and Firefly...Combines in vivo BRET with ex vivo luminescence-based co-immunoprecipitation to assess protein-protein interactions...upstream of Firefly luciferase. Renilla luciferase is co-expressed. Oskar Laur 87070 pcDNA3.1-Nanoluc-ccdB...Firefly luciferase fusion for expression in plants, co-expressed with mScarlet-I for ratiometric imaging...C-terminal Red Luciferase fusion for expression in plants, co-expressed with Gaussia luciferase for ratiometric...

...etc.) On the other hand, gRNA plasmids that do not co-express a Cas protein can be paired with a wide variety...Plasmid Promoter Cloning Enzyme(s) Delivery Resistance Co-expressed Cas9 Depositing lab Cas9 species = S. pyogenes...

...etc.) On the other hand, gRNA plasmids that do not co-express a Cas protein can be paired with a wide variety...Promoter Cloning Enzyme(s) Validated In Resistance Co-expressed Cas9 Depositing lab Cas9 species = S. pyogenes...

...Link opens in a new window) Seydoux lab website . Co-CRISPR plasmids that facilitate detection of genome-editing...Developed in Craig Mello's lab and described in A Co-CRISPR Strategy for Efficient Genome Editing in Caenorhabditis...

...neuronal co-injection marker); pCFJ104 (body wall muscle co-injection marker); pCFJ90 (pharyngeal co-injection...

...etc.) On the other hand, gRNA plasmids that do not co-express a Cas protein can be paired with a wide variety...Promoter Cloning Enzyme(s) Validated In Resistance Co-expressed Cas9 Depositing lab Cas9 species = S. pyogenes...

...system, Cas9 is tagged with a biotin acceptor site and co-expressed with BirA biotin ligase. The locus is isolated...etc.) On the other hand, gRNA plasmids that do not co-express a Cas protein can be paired with a wide variety...

...etc.) On the other hand, gRNA plasmids that do not co-express a Cas protein can be paired with a wide variety...Cas-containing plasmids. ID Plasmid Promoter Cloning Enzyme Co-expressed Cas9 Cas9 System Selection PI 46968 pICSL01009...

...achieved by fusing a biotin acceptor site to dCas9 and co-expressing BirA biotin ligase, as seen in the CAPTURE...

...non-repetitive genomic locus can also be labeled by co-delivering multiple gRNAs that tile the locus. Chromosome...

...Cas system can be implemented in mammalian cells by co-expressing the S. pyogenes Cas9 (SpCas9) nuclease...expressing human codon-optimized SpCas9; sgRNA can be co-transfected as PCR amplicons (Ran et al., Nature ...human codon-optimized SaCas9; SaCas9 sgRNA can be co-transfected as PCR amplicons using a protocol similar...

...112006-AAV9 pAAV-hSynapsin1-GCaMP6s-P2A-mKate2 For co-expression of GCaMP6s with mKate2 Lin Tian 112007...112007-AAV9 pAAV-hSynapsin1-GCaMP6s-P2A-mRuby3 For co-expression of GCaMP6s with mRuby3 Lin Tian 112010-AAV5...combination with Cre-dependent transgenic mouse lines or co-infection with Cre dependent viral vectors. Jonathan...-hSyn-DIO-jGCaMP8s-P2A-ChrimsonR-ST Cre dependent co-expression of jGCaMP8s and soma-targeted ChrimsonR...

...ability to bind to methylated DNA and recruit known co-repressors or other transcriptional modifiers suggests...D omain (TRD) the TRD contains a Nuclear receptor Co-Repressor 1/Silencing Mediator of Retinoic acid and...

... recombinant antibody preps contain low levels of co-purified plasmid from transfection. This residual...