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We narrowed to 6 results for: turboid

Showing: 1 - 6 of 6 results

  1. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

    Type
    Blog Post
    Published
    Dec. 2, 2019, 2 p.m.
    ...APEX2 plasmids! TurboID and minturbo: Rapid and non-toxic proximity labeling  TurboID and miniTurbo were...directed evolution to generate two mutants, the 35 kD TurboID, which has 15 mutations compared to BirA and the...deleted and 13 mutations compared to wild type BirA. TurboID and miniTurbo both enable biotin labeling in 10...10 minutes without any issues in toxicity. TurboID’s signal is significantly greater than miniTurbo at...more precise temporal control.  Find plasmids for TurboID and miniturbo Split-BioID: A conditional proteomics...proximity labeling in living cells and organisms with TurboID." Nature biotechnology 36.9 (2018): 880. PubMed...

  2. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

    Type
    Blog Post
    Published
    Dec. 6, 2018, 1:34 p.m.
    ...labeling with TurboID and miniTurbo Article contributed by Eric Perkins  Listen to the TurboID and miniTurbo...recent Nature Biotechnology paper, they describe TurboID and miniTurbo, which utilize non-toxic biotin, ... optimally at 37℃. The increased efficiency of TurboID and miniTurbo makes PL a viable method for proteomics...including Drosophila and C. elegans.   Find the TurboID plasmids here! Branon et al. Nat Biotechnol. 2018...

  3. Hot Plasmids - May 2022

    Type
    Blog Post
    Published
    May 26, 2022, 1:15 p.m.
    ...to its target stabilizes the TurboID-nanobody protein and allows TurboID to start labeling proteins in... al. 2021.    BLITZ uses the biotin ligase TurboID fused to a conditionally stabilized GFP-nanobody...

  4. Hot Plasmids - November 2023

    Type
    Blog Post
    Published
    Nov. 14, 2023, 3:35 a.m.
    ...engineering a light-sensitive LOV domain into the TurboID enzyme, Lee and Cheah et al. achieved precise control...TransitID, where two proximity labeling enzymes, TurboID and APEX2, are localized to different cellular ...proteins. B) TransitID uses sequential labeling by TurboID and APEX2 at different cellular locations. C) Enrichment...

  5. Hot Plasmids: Spring 2025

    Type
    Blog Post
    Published
    June 3, 2025, 1:15 p.m.
    ... Monroe, MS. Proximity ligation tools like TurboID and its mini counterpart label nearby proteins ...proteins at a certain time and genomic location. TurboID improved on other proximity ligation tools with...proximity labeling in living cells and organisms with TurboID. Nature Biotechnology, 36(9), 880–887. https://... of dCas9 with the dynamic labeling power of miniTurboID, a proximity ligation tool (Cenik et al., 2024...kinetics and less background labeling. By fusing miniTurboID with dCas9 to create TurboCas, the Shilatifard...
Showing: 1 - 6 of 6 results