...premises we can classify a plasmid preparation in 3 different ways: Transformation grade DNA Cloning grade ... our Plasmids 101 blog posts Learn about the differences between transformation, transduction, conjugation...Resources on Addgene.org Read the molecular biology reference Find plasmids for your research Brush up on molecular...

...but when it came time to add author details a different person in the lab wanted to be the first author...

...beginning of September all will be powered by 100% renewable electricity! A (not so) shocking transition  ...bulk purchase energy from a higher percentage of renewable sources. This program offers options of between...between 14% - 100% electricity supplied from renewable sources and we have chosen to participate at the highest... will be drawing 100% of our electricity from renewable energy projects right here in New England- the...attempt to save energy. The transition to 100% renewable electricity will allow us to maintain these particularly...the office more eco-friendly The switch to 100% renewable electricity is certainly not the only sustainability...as well. For a more in-depth look at Addgene’s current lab-oriented environmental efforts check out Leila...

...comparing Addgene global deposit and distribution trends for Zinc Finger, TALE, CRISPR, and SynBio plasmids...

...Before the conference Is the conference you are attending on the larger side? At large conferences, chances...once in different rooms. Before arriving at the conference, plan out your agenda. Some conferences even ... the entire conference, in general). After the conference Review your favorite conference moments on social...present your findings at a conference. But what you get out of a conference depends on what you put into...introduction. During the conference When you meet someone new at a conference, one of the first things... the conference, you can end by asking them to come to your poster or talk. After the conference After...elevator pitch several times at the conference, you might notice some trends. Were there parts of your elevator...

... of the conference Addgenies were lucky enough to meet hundreds of the over 11,000 conference registrants...Check Out Our Microbiology Page! The start of the conference After setting up the Addgene booth on Thursday...involved with was one of the highlights of the conference. It was inspiring to see how microbiology research...were happy to chat with lots of researchers who weren’t aware of Addgene’s plasmid sharing services, as...out many of the posters being presented at the conference and talked to some scientists who stopped by ...from the nearly 4000 posters presented at the conference, it looks like there are plenty more exciting...Engineering Learn how to Network at Scientific Conferences Resources on Addgene.org Check out Our Microbiology...

...Research" conference. You can register for the conference here. The Future of Research conference is organized...training. One of the best recent discussions of this apparently worsening crisis recently appeared in eLife: ...

...public, not private interests. On this nonprofit awareness day, we layout the ways in which we promote our...build reserves so the company is sustainable. The difference is what nonprofits do with their revenue. A nonprofit... offices trust us to work for them with full transparency. We don’t have to explain that the materials...collaboration, and fun Addgene believes there is strength in diversity and in empowering team members to... Jane, Jason, Jessica, Joel, Jonathan, Justine, Karen, Kate, Kayla, Kelly, Kory, Kris, Leila, Lianna, ...Pamela, Racheal, Ramsey, Randall, Ray, Rebecca, Renee, Shannon, Susanna, Tenisha, Thomas, Tony, Tracy,... nonprofit awareness day...

...types in the body from otherwise terminally differentiated cells simply by expressing four proteins Oct4... usually I only get to scratch the surface of different fields. This meeting has given me an opportunity...can often take the back burner at scientific conferences. In my nearly 20-year career in science, I have...Shinya Yamanaka, George Q. Daley, Christine Mummery, Lorenz Studer, and Deepak Srivastava--addressed a battery...trial patients with advanced macular degeneration; Laurenz Studer discussed his work with Parkinson's Disease...

...revolutionizing your research. Why serendipity matters Serendipity is not just a stroke of luck; it's ...redefine the course of one's research journey — serendipity. The best scientific breakthroughs often occur...delve into the importance of leaving room for serendipity during your PhD studies and offer two simple ... your search The first step towards inviting serendipity into your research lies in expanding the scope...from looking beyond its confines. The beauty of serendipity often emerges at the intersections of disciplines...sparking innovative thinking that may benefit your current project. Another strategy could entail immersing...Widening your search creates fertile ground for serendipitous encounters that could spark unforeseen connections...

... the conference, map out which posters you are interested in viewing. For most larger conferences, poster...ensure you have the most beneficial scientific conference experience possible. Tips for poster session ... times while standing at your poster – it’s a different experience to practice while seated or with your...Dress for the venue and professionalism of the conference, and remember to wear comfortable shoes, as you...your poster board well ahead of time ensures you aren’t scrambling to tack up your poster seconds before...this, and have many times answered a completely different question than the intended one. Take a deep breath...tips and tricks for presenting at scientific conferences, so let us know your own ways to successfully...

...second day of the conference included workshops which were framed in advance by the conference organizing team...the amazing, postdoc-driven Future of Research Conference at Boston University last week.  This fantastic...past practices and mores.  His list is dire:  The current system is unsustainable, hypercompetition is corroding...cited the oft quoted statistic that only 15% of current postdocs will be academic principal investigators...

...spending on a conference? One thing to consider is the size of the conference. Large conferences have many...make new relationships than at scientific conferences. Conferences provide the opportunity to meet people...especially when solving problems.  During the conference The conference may start in the airport. If you see ...programs. After the conference Follow up with the new people you met at the conference. If you promised ...overwhelming as far as meeting new people. At large conferences, look for smaller events or meet-ups to connect...sessions that match your interests. I favor small conferences where the attendees stay onsite and eat together...face it, you see enough of them).  Before the conference Make sure you have a business card – even students...

...sessions The poster session from conference to conference can be wildly different. There can be no presenter...participants used the conference hashtag after the conference was over. Depending on the conference, organizers ...scientific conferences. Plan your sessions, not your walks between sessions Like many large conferences with...can use to help plan your conference experience.One of the biggest differences: you don't have to build..., requiring you to zoom in to different parts of the poster. Conferences, such as Photonics Online Meetup..., 2020 Final thoughts on virtual conferences Virtual conferences remove many barriers of participation...spend with the conference. “If you want to have the same experience as an on-site conference, you really ...

...enzymes and these different Dicer-like enzymes are involved in the biogenesis of different small RNAs (Figure...choice of tool requires careful balancing of the inherent advantages and limitations of any technique in...that function downstream of DNA, such as RNA interference, can reversibly exert their effect independent...independent of gene copy number. RNA interference (RNAi) is a conserved eukaryotic process where approximately...transcriptionally or post-transcriptionally. In plants, different dicer-like enzymes (DCL) process the dsRNA into...equivalently precise, but each method recovered different genes known to be required for viability (Morgens...subsequent two. Consequently, I will focus on different approaches and considerations present at the first...

...freezers that the samples can be stored in? Different shelves? Different racks on each shelf? Give each container...about location, location, location There are many different types of samples at Addgene - DNA sent from depositors... that we never run out. There are a number of different strains of E. coli that can be used for transformation... concentration or need to be transformed into different cells than were originally requested. Most of ...analysis from our protocols and molecular biology reference pages! Resources Avaliable on the Addgene Blog...

...green cards and citizenship.   Lauren Celano, CEO Propel Careers. Lauren is passionate about working with...This post was contributed by guest bloggers Lauren Celano of Propel Careers and Rachel Casseus, Esq. ...information presented may not reflect the most current legal developments.  Further, this post may contain...development for foreign national researchers and entrepreneurs who are looking to continue their careers in...tasked with working with US immigration in its current state. As of September 2017, USCIS received 116,224... As an international researcher and aspiring entrepreneur, you have many career options available to you...skills overtime for success. Many people will have different roles, titles, and responsibilities during their...

...We’re excited to welcome Karen Katz, Esq and Carl Paratore, MBA, CPA to the Addgene Board of Directors...scientific community manage those plasmids so scientists aren’t bogged down with distributing them and spending... enjoying that immensely. Paratore: I have two children active in sports, so I enjoy watching their games... Daisy, or out on golf course! Learn more about Karen Katz, Esq Ms. Katz is the Director of Intellectual...Property Programs at Suffolk University Law School. Karen graduated with highest honors from the University...associated with Hale & Dorr and Crowell & Morning. Karen was a founding member of Mass Medical Angels, a ...

...codon usage biases of different organisms. Different species have different preferences for the codons they...scientists from MIT tapped into this data to learn about trends in synthetic biology and DNA synthesis. They published...the gene may not express well because of these different codon usage biases. But if a gene’s sequence was...could be determined through comparisons with a reference sequence database. Through a series of simulations...Kunjapur says. Percent sequence identity was a different story - the team found that the percent sequence...sequences in Addgene’s database. They showed a growing trend of sourcing genes from distantly related organisms...and synthetic biology is expanding rapidly.  References 1. Kunjapur, Aditya M., Philipp Pfingstag, and...

...Koeris' own transition from graduate student to entrepreneur, listen to our full interview with Dr. Koeris...contrasts being a graduate student with being an entrepreneur 29:00 - 32:20 : What did It mean for Dr. Koeris...developing a diagnostic? 36:53 - 38:00 : As an entrepreneur, is it more important to develop something that...Board and how he hopes to help young biotech entrepreneurs.   We'd like to thank Dr. Koeris for taking... entrepreneur...