.... Find pSpCas9(BB)-2A-Puro (PX459) V2.0. pCMV-VSV-G - pCMV-VSV-G was deposited by the Bob Weinberg lab.... It can be used with packaging plasmids such as pCMV-dR8.2 dvpr (lentiviral) or pUMVC (MuLV retroviral..., this plasmid predates Addgene’s founding. Find pCMV-VSV-G. pRSV-Rev - This 3rd generation lentiviral...15 years of plasmid sharing has certainly taken us on many adventures...From moving office locations ...plasmids are from the Zhang lab. In fact, the Zhang lab has shared over 40,000 CRISPR-Cas9 components over the...Chimeric_BB-CBh-hSpCas9 - The S. pyogenes Cas9 (SpCas9) has been widely popular in genome engineering. For use...

... base editor pCMV-BE3 has been requested nearly 400 times, and newcomer pCMV-ABE7.10 has already earned... CRISPR has made it possible to design programmable epigenome editing tools, and the field has certainly...Since the start of the CRISPR revolution, Addgene has distributed over 100,000 CRISPR plasmids. But that...have available by category. Each plasmid page now has a helpful graphic to introduce you to the plasmid... the Transcriptional Regulation category already has five blue flame plasmids covering three different...LwCas13a guide expression backbone with U6 promoter has already earned a blue flame. RNA Editing: Did anyone...

... Protoc. 2017 with modifications Screening pCMV-Rep78/68 Scanning Saturation Mutagenesis (SSM) Pooled...1866 bp, plus a 20 bp barcode 400 ng Solid pCMV-Rep 78/68 SSM Library Amplification and Sequencing...replicate faster. For this reason, if your library has inserts of different sizes (such as a transcription...

...CMV-mito-GEM-GECO1  and blue flame extracellular glutamate sensor pCMV(MinDis).iGluSnFR. Picking your reporter Don’t forget...enzyme activity. Small molecule biosensors Addgene has many sensors that measure the concentration of small...

.... Grant Challen’s lab also created constitutive (pCMV-dCas9-D3A) and Tet-dependent (TetO-dCas9-D3A) constructs...sum of epigenetic modifications across the genome) has been implicated in disease states, and targeting ...genomic loci is possible using CRISPR, and Addgene has a number of tools for this purpose. Epigenetics began...many TALEN-based technologies, the advent of CRISPR has made targeting much easier! With the help of guide...modification at specified loci. Charles Gersbach’s lab has deposited mammalian expression constructs including...BioRender.com. Tet1 demethylase Ronggui Hu’s lab has created pdCas9-Tet1-CD for targeted cytosine demethylation...Methyltransferase 3 Alpha (DNMT3A) Vlatka Zoldoš’ lab has deposited pdCas9-DNMT3A-EGFP and pdCas9-DNMT3A-PuroR...

...MbPyl pyrrolysyl-tRNA synthetase Mammalian Cells pCMV-DnpK dinitrophenyl hapten Mammalian Cells pANAP..., organelles or other cellular impediments. FRET has been used in many applications, including drug discovery...up a FRET-based assay or screen (the Addgene blog has an excellent primer on FRET and how to measure it...synthetases is required and thus far, this method has proven most effective only in a few cell types (E...FRET measurements in intact cells. The Gradia Lab has deposited a gateway vector with Addgene, where you...

... activity; protein purification vector  87439  pCMV-HF-BE3  HF-Cas9 (NGG)  Very low off-target activity...inhibitory GABA-ergic interneurons, Gordon Fishell’s lab has developed a new AAV approach using regulatory elements...fluorescent protein - mScarlet! The Dorus Gadella lab has recently developed a synthetic red fluorescent protein... imaging and in protein fusions. The Gadella lab has deposited all mScarlet constructs with Addgene. ...efficiency Blue flame depositor Yannick Doyon’s lab has developed a new method to increase CRISPR editing...increases the probability that the locus of interest has also been edited! Thus, ouabain selection enriches...coselection are available at Addgene, and the Doyon lab has also provided a detailed protocol at Protocol Exchange...

... plasmid, pCMV14-3X-Flag-SARS-CoV-2 S, which is now easily available through Addgene and has already been...Jackson Laboratory, a nonprofit research institution, has been able to rapidly propagate this strain, making...