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  1. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    Published
    May 4, 2017, 2:30 p.m.
    ...previously developed monomeric fluorescent proteins mKO and mCherry, respectively. While Tsuboi et al developed...somewhat serendipitously while attempting to enhance mKO in other ways, Subach et al made a concerted effort...

  3. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type
    Blog Post
    Published
    June 15, 2017, 2:30 p.m.
    ...can be used in the OSER assay including CytERM-mKOkappa, CytERM-mTurquoise2, and CytERM-dTomato. When...structure. Based on these data, we conclude that mKOk has dimerization tendency and is not suitable for...

  4. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type
    Blog Post
    Published
    July 24, 2018, 12:51 p.m.
    ...Transfer Vectors   Expressing your gene of interest pMKO.1 GFP (Plasmid #10676) Retroviral vector for cloning...with GFP expression.  Cloning and expressing RNAi pMKO.1 puro (Plasmid #8452) RNA interference (RNAi) is...

  5. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    Published
    Nov. 12, 2020, 2:15 p.m.
    ...ability to selectively silence neurons in vivo (Slimko and Lester, 2003).  Addgene has a large selection...1281–1295 . https://doi.org/10.1093/nar/15.3.1281 Slimko EM, Lester HA (2003) Codon optimization of Caenorhabditis...

  7. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    Published
    July 16, 2015, 2:30 p.m.
    ...Leipzig: S. Hierzel.
 J.A Dent, A.G. Polson, M.W. Klymkowsky. (1989) Development, 105.61-74. Pubmed. J. Sharpe...

  8. 15 Hot Plasmids from 2017

    Type
    Blog Post
    Published
    Jan. 9, 2018, 3:02 p.m.
    ...for mTagBFP, TagRFPt, EGFP, mVenus, mCerulean3, mKOFP2) and empty vectors that have standard cloning sites...
Showing: 1 - 8 of 8 results