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Showing: 1 - 15 of 15 results
  1. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    ...previously developed monomeric fluorescent proteins mKO and mCherry, respectively. While Tsuboi et al developed...somewhat serendipitously while attempting to enhance mKO in other ways, Subach et al made a concerted effort...
  2. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...mKusabira Orange-C1 (mKO) - Mammalian Expression mKO-pBAD - Bacterial Expression mOrange 548 562 49 6.5... Plasmids mKusabira-Orange (mKO) 548 559 31 5 4.5 hr Monomer pQC mKorange IX - Mammalian Expression mKusabira...Expression mKO2 551 565 40 5.5 Monomer mKO2-N1 - Mammalian Expression mKO2-C1 - Mammalian Expression mKO2-pBAD...
  3. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    ...ability to selectively silence neurons in vivo (Slimko and Lester, 2003).  Addgene has a large selection...1281–1295 . https://doi.org/10.1093/nar/15.3.1281 Slimko EM, Lester HA (2003) Codon optimization of Caenorhabditis...
  4. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...for mTagBFP, TagRFPt, EGFP, mVenus, mCerulean3, mKOFP2) and empty vectors that have standard cloning sites...
  5. Neurodegeneration Plasmid Collection

    Type
    Collection
    ... pCMV-mKO2-Parkin PRKN mKO2 CMV Parkinson's Lani Wu 213545 pCMV-Parkin(C431N)-A92mKO2 PRKN mKO2 CMV Parkinson's...pCMV-Parkin-A92mKO2 PRKN mKO2 CMV Parkinson's Lani Wu 213549 pLv-CMV(trunc)-Parkin-A92mKO2 PRKN mKO2 CMV Parkinson's...K161N)-A92mKO2 PRKN mKO2 EF1a Parkinson's Lani Wu 213555 pLv-EF1a-Parkin(N273K)-A92mKO2 PRKN mKO2 EF1a ...R104A)-A92mKO2 PRKN mKO2 EF1a Parkinson's Lani Wu 213557 pLv-EF1a-Parkin(S65A)-A92mKO2 PRKN mKO2 EF1a Parkinson's...pLv-EF1a-Parkin(W403A)-A92mKO2 PRKN mKO2 EF1a Parkinson's Lani Wu 213559 pLv-EF1a-Parkin-A92mKO2 PRKN mKO2 EF1a Parkinson's...Parkinson's, FTD Michael Davidson 57883 mKO2-MAPTau-C-10 MAPT mKO2 CMV Parkinson's, FTD Michael Davidson...
  6. Mammalian RNAi Tools

    Type
    Collection
    ...vector for pMKO.1 puro; Contains shRNA against GFP William Hahn 10676 pMKO.1 GFP Derivative of pMKO.1 puro...Description PI 8452 pMKO.1 puro Retroviral vector for shRNA expression Bob Weinberg 10675 pMKO.1 puro GFP shRNA...
  7. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ... Tet-inducible lentiviral shRNA expression pMKO.1 puro - Retroviral shRNA expression... GFP - Retroviral gene expression pMKO.1 GFP - Retroviral shRNA expression... expression, multiple selectable markers pMKO.1 puro - Mammalian retroviral shRNA...plasmid for mammalian gene silencing pMKO.1 puro - Retroviral plasmid for mammalian...
  8. Retrovirus Plasmids

    Type
    Collection
    ...Weinberg 10676 pMKO.1 GFP MoMLV U6-driven plasmid for shRNA expression; also expresses GFP. See pMKO.1 puro for...
  9. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...Weissman 3rd 5 10 107,415 214,830 DNA Damage Response MKOv4 Library 140219 Knockout Human J. Chen 3rd 10 4,530...
Showing: 1 - 15 of 15 results