...the Addgene repository in 2015 was... lentiCRISPR V2   LentiCRISPR v2 originates from Feng Zhang’s lab... and how does it work? LentiCRISPR v2 purpose As its name suggests, lentiCRISPR v2 is a lentiviral transfer...,000 human and mouse genes respectively, use lentiCRISPR v2 as the backbone for their many gRNAs. The ...high titer lentivirus production afforded by lentiCRISPR v2 is key to its efficient use in screening experiments...pooled library screens. Availability and Use LentiCRISPR v2 is available from Addgene both as a single...CRISPR/Cas9. If you need help cloning a gRNA into lentiCRISPR v2, you can take a look at the protocol kindly...kindly provided by the Zhang lab. Even though lentiCRISPR v2 has only been available for a little over a ...

...transfected cells. Find pSpCas9(BB)-2A-GFP (PX458). lentiCRISPR v2 - This 3rd generation lentiviral transfer ...the top requested plasmid of 2015. What makes lentiCRISPR v2 great? It produces a ~10 fold higher titer...CRISPR pooled library screening experiments. Find lentiCRISPR v2.(If you’ve peeked through the rest of the ...

...the Addgene viral service. CRISPR technology lentiCRISPR v2 (Plasmid #52961) This is a 3rd generation ...titer of lentiviral particles than the original lentiCRISPR v1. Special note from the Zhang lab: We are constantly...

...including Addgene’s top requested plasmid in 2015: lentiCRISPR v2, used both for targeted modification and genome-wide...