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Showing: 1 - 9 of 9 results

  1. Your Top Requested Plasmid in 2015!

    Type
    Blog Post
    ...the Addgene repository in 2015 was... lentiCRISPR V2   LentiCRISPR v2 originates from Feng Zhang’s lab... and how does it work? LentiCRISPR v2 purpose As its name suggests, lentiCRISPR v2 is a lentiviral transfer...,000 human and mouse genes respectively, use lentiCRISPR v2 as the backbone for their many gRNAs. The ...high titer lentivirus production afforded by lentiCRISPR v2 is key to its efficient use in screening experiments...pooled library screens. Availability and Use LentiCRISPR v2 is available from Addgene both as a single...CRISPR/Cas9. If you need help cloning a gRNA into lentiCRISPR v2, you can take a look at the protocol kindly...kindly provided by the Zhang lab. Even though lentiCRISPR v2 has only been available for a little over a ...

  2. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    ...transfected cells. Find pSpCas9(BB)-2A-GFP (PX458). lentiCRISPR v2 - This 3rd generation lentiviral transfer ...the top requested plasmid of 2015. What makes lentiCRISPR v2 great? It produces a ~10 fold higher titer...CRISPR pooled library screening experiments. Find lentiCRISPR v2.(If you’ve peeked through the rest of the ...

  4. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    ...including Addgene’s top requested plasmid in 2015: lentiCRISPR v2, used both for targeted modification and genome-wide...

  5. CRISPR References and Information

    Type
    Collection
    ...D10A (pX335) ; PDF 248.6 KB Zhang sgRNA cloning lentiCRISPR v2 packaging plasmids: pVSVg , psPAX2 ; positive...pooled library amplification 1 vector system: lentiCRISPR v2 2 vector system: lentiCas9-Blast and lentiGuide-Puro...

  6. CRISPR Pooled gRNA Libraries

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    Collection
    ...Varies 156,250 Mouse CRISPR Knockout Library in lentiCRISPR v2-Blast 1000000173 Knockout Mouse Liu 3rd 10...

  7. Zhang Lab CRISPR Page

    Type
    Collection
    ... available in two formats: 1 vector system - lentiCRISPR - sgRNA and SpCas9 together 2 vector system -...

  9. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Familial British dementia Jan Johansson 138347 lentiCRISPR v2-sgDnmt1_1 DNMT1 U6 Hereditary sensory neuropathy...neuropathy type IE Yi Zhang 138348 lentiCRISPR v2-sgDnmt1_2 DNMT1 U6 Hereditary sensory neuropathy type ...LRRK2 His T7 Parkinson's Suzanne Pfeffer 186025 lentiCRISPR v2-sgCOQ2 COQ2 U6 Multiple system atrophy Boyi...
Showing: 1 - 9 of 9 results