...later generations of GCaMP6, to the service. While they were both requested, GCaMP6 remains the more requested...with successive generations developed ever since. GCaMP6 was offered through our viral vector service in...than jGCaMP7 (Figure 5B).   Figure 5: A) GCaMP6, jGCaMP7, and jGCaMP8 viral preps distributed per...2016–2023. B) Inset showing distribution of only GCaMP6, jGCaMP7, and jGCaMP8 per year from 2016–2023. ..., as jGCaMP7 was released relatively soon after GCaMP6. It could be a matter of the size of the incremental... “picture” of a train of action potentials that GCaMP6 and jGCaMP7 do not. Most likely, it’s a combination...

...to have slower, but larger, calcium changes. The GCaMP6 family of sensors are commonly used calcium reporters... plasma membrane. GCaMP-X is a nucleus-targeted GCaMP6 with an extra apoCaM-binding motif to prevent aberrant...expression in both zebrafish and mammalian systems. The GCaMP6, jGCaMP7, and jGCaMP8 sensors are also suitable...optimized for neuron-specific, astrocyte-specific (GCaMP6 only) or mammalian cell expression, and compatible...specific to the neuronal cell body or the axon (GCaMP6 only). The genetic elements required for Drosophila...

...AAV1 pAAV-hSynapsin1-axon-GCaMP6s 112005  AAV9 pAAV-hSynapsin1-axon-GCaMP6s-P2A-mRuby3) 112010  AAV9...AAV9 pAAV-hSynapsin1-FLEx-axon-GCaMP6s New voltage sensors Plasmid Serotype Name 119036  AAV1  ...variant 1513-WPRE 105714  AAV8  pAAV-Ef1a-fDIO-GCaMP6s Optogenetics Plasmid Serotype Name 124651...

...axon-GCaMP6s-P2A-mKate2 (AAV9) pAAV-hSynapsin1-GCaMP6s-P2A-mKate2 (AAV9) pAAV-hSynapsin1-GCaMP6s-P2A-mRuby3...dLight1.2 and voltage sensor Ace2N and additional GCaMP6 constructs: pAAV-CAG-dLight1.2 (AAV1) pGP-AAV-...-mRuby3 (AAV9) pAAV-CAG-SomaGCaMP6f2 (AAV9) Plus, additional AAV serotypes of the most recent jGCaMP8...

...viral prep in serotype AAV1. Synapse targeting GCaMP6s from the Rylan Larsen lab are now available in ...ready-to-use AAV5 viral vector. Synaptophysin-tagged GCaMP6s is enriched in the pre-synaptic terminal providing...expressing GCaMP6f with this promoter. Image calcium transients in PV interneurons with AAV-S5E2-GCaMP6f are ...viral preps from recent months: Axon targeting GCaMP6f from Lin Tian’s lab is available in serotype AAV1...

...optogenetics, and other imaging tools. The GCaMP5 and GCaMP6 series of genetically encoded calcium indicators...

...GP-AAV-CAG-FLEX-jGCaMP7s-WPRE 105714 AAV8 pAAV-Ef1a-fDIO-GCaMP6s Neurotransmitter sensors: Dopamine and norepinephrine...

...variant, and is approximately 5-fold brighter than GCaMP6s. jGCaMP7f is the fastest responding variant. jGCaMP7b...

...cytoplasmic-tdTomato and Synaptophysin-GFP. Synapse targeting GCaMP6s from the Rylan Larsen lab, now available in a ready...calcium transients in PV interneurons with AAV-S5E2-GCaMP6f, now available as a ready-to-use viral prep in ...

...expresses a genetically encoded calcium sensor, GCaMP6 (Nguyen et al., 2015). If you are interested in...

... sparse (e) labeling of the calcium indicator, GCaMP6f, in the Drosophila optic lobe. Image from Isaacman-Beck...of T5 neurons by placing the calcium indicator GCaMP6f in the effector position, as well as to optogenetically...

... sparse (e) labeling of the calcium indicator, GCaMP6f, in the Drosophila optic lobe. Image from Isaacman-Beck...

... Plasmid Serotype Name 83899 AAVrg pAAV-mDlx-GCaMP6f-Fishell-2  Additional resources on the Addgene...

...mDlx-GFP, hDlx-FLEX-GFP, hDlx-Flex-dTomato, mDlx-GCaMP6f, hDlx-GiDREADD-dTomato and mDlx-ChR2-mCherry. ...