We narrowed to 14 results for: gcamp6
-
TypeBlog PostPublishedJuly 30, 2024, 1:15 p.m....later generations of GCaMP6, to the service. While they were both requested, GCaMP6 remains the more requested...with successive generations developed ever since. GCaMP6 was offered through our viral vector service in...than jGCaMP7 (Figure 5B). Figure 5: A) GCaMP6, jGCaMP7, and jGCaMP8 viral preps distributed per...2016–2023. B) Inset showing distribution of only GCaMP6, jGCaMP7, and jGCaMP8 per year from 2016–2023. ..., as jGCaMP7 was released relatively soon after GCaMP6. It could be a matter of the size of the incremental... “picture” of a train of action potentials that GCaMP6 and jGCaMP7 do not. Most likely, it’s a combination...
-
Viral Vectors 101: Calcium Sensors
TypeBlog PostPublishedAug. 10, 2023, 1 p.m....to have slower, but larger, calcium changes. The GCaMP6 family of sensors are commonly used calcium reporters... plasma membrane. GCaMP-X is a nucleus-targeted GCaMP6 with an extra apoCaM-binding motif to prevent aberrant...expression in both zebrafish and mammalian systems. The GCaMP6, jGCaMP7, and jGCaMP8 sensors are also suitable...optimized for neuron-specific, astrocyte-specific (GCaMP6 only) or mammalian cell expression, and compatible...specific to the neuronal cell body or the axon (GCaMP6 only). The genetic elements required for Drosophila... -
New and Upcoming Viral Vectors - June 2019
TypeBlog PostPublishedJune 18, 2019, 1:15 p.m....AAV1 pAAV-hSynapsin1-axon-GCaMP6s 112005 AAV9 pAAV-hSynapsin1-axon-GCaMP6s-P2A-mRuby3) 112010 AAV9...AAV9 pAAV-hSynapsin1-FLEx-axon-GCaMP6s New voltage sensors Plasmid Serotype Name 119036 AAV1 ...variant 1513-WPRE 105714 AAV8 pAAV-Ef1a-fDIO-GCaMP6s Optogenetics Plasmid Serotype Name 124651... -
Hot Biosensors 2022: Year-End Roundup
TypeBlog PostPublishedDec. 6, 2022, 2:15 p.m....axon-GCaMP6s-P2A-mKate2 (AAV9) pAAV-hSynapsin1-GCaMP6s-P2A-mKate2 (AAV9) pAAV-hSynapsin1-GCaMP6s-P2A-mRuby3...dLight1.2 and voltage sensor Ace2N and additional GCaMP6 constructs: pAAV-CAG-dLight1.2 (AAV1) pGP-AAV-...-mRuby3 (AAV9) pAAV-CAG-SomaGCaMP6f2 (AAV9) Plus, additional AAV serotypes of the most recent jGCaMP8... -
Hot Plasmids and Viral Preps - May 2021
TypeBlog PostPublishedMay 18, 2021, 1:15 p.m....viral prep in serotype AAV1. Synapse targeting GCaMP6s from the Rylan Larsen lab are now available in ...ready-to-use AAV5 viral vector. Synaptophysin-tagged GCaMP6s is enriched in the pre-synaptic terminal providing...expressing GCaMP6f with this promoter. Image calcium transients in PV interneurons with AAV-S5E2-GCaMP6f are ...viral preps from recent months: Axon targeting GCaMP6f from Lin Tian’s lab is available in serotype AAV1... -
The 10 Most Distributed Plasmid Technologies in Addgene's First 10 Years
TypeBlog PostPublishedJan. 8, 2014, 3:10 p.m....optogenetics, and other imaging tools. The GCaMP5 and GCaMP6 series of genetically encoded calcium indicators... -
New and Upcoming Viral Vectors - December 2019
TypeBlog PostPublishedDec. 19, 2019, 2:40 p.m....GP-AAV-CAG-FLEX-jGCaMP7s-WPRE 105714 AAV8 pAAV-Ef1a-fDIO-GCaMP6s Neurotransmitter sensors: Dopamine and norepinephrine... -
New and Upcoming Viral Vectors - September 2019
TypeBlog PostPublishedSept. 12, 2019, 1 p.m....variant, and is approximately 5-fold brighter than GCaMP6s. jGCaMP7f is the fastest responding variant. jGCaMP7b... -
Hot Plasmids and Viral Preps - March 2021
TypeBlog PostPublishedMarch 23, 2021, 1:15 p.m....cytoplasmic-tdTomato and Synaptophysin-GFP. Synapse targeting GCaMP6s from the Rylan Larsen lab, now available in a ready...calcium transients in PV interneurons with AAV-S5E2-GCaMP6f, now available as a ready-to-use viral prep in ... -
Five Popular Model Organisms
TypeBlog PostPublishedApril 11, 2019, 12:39 p.m....expresses a genetically encoded calcium sensor, GCaMP6 (Nguyen et al., 2015). If you are interested in... -
Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation
TypeBlog PostPublishedMay 21, 2020, 1:15 p.m.... sparse (e) labeling of the calcium indicator, GCaMP6f, in the Drosophila optic lobe. Image from Isaacman-Beck...of T5 neurons by placing the calcium indicator GCaMP6f in the effector position, as well as to optogenetically... -
Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative
TypeBlog PostPublishedJune 2, 2020, 1:15 p.m.... sparse (e) labeling of the calcium indicator, GCaMP6f, in the Drosophila optic lobe. Image from Isaacman-Beck... -
New and Upcoming Viral Vectors - May 2020
TypeBlog PostPublishedMay 28, 2020, 1:15 p.m.... Plasmid Serotype Name 83899 AAVrg pAAV-mDlx-GCaMP6f-Fishell-2 Additional resources on the Addgene... -
Hot Plasmids - August 2020
TypeBlog PostPublishedAug. 18, 2020, 1:15 p.m....mDlx-GFP, hDlx-FLEX-GFP, hDlx-Flex-dTomato, mDlx-GCaMP6f, hDlx-GiDREADD-dTomato and mDlx-ChR2-mCherry. ...