Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis
Type
Blog Post
Published
Aug. 28, 2014, 3:34 p.m.
...was digested with 2 unique enzymes (HindIII and BamHI) and run on an agarose gel. The resulting gel image...the plasmid is digested with either HindIII and BamHI alone (lanes 4-5), there is a single band of 7.3...plasmid. The double digest with both HindIII and BamHI (lane 3) produces bands at 6kb and 1.2kb (red box... star activity. Some endonucleases (for example BamHI) are capable of cleaving sequences which are similar...