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We narrowed to 10 results for: SunTag

Showing: 1 - 10 of 10 results

  1. SunTag and Fluorescent Imaging

    Type
    Blog Post
    Published
    March 28, 2017, 2:30 p.m.
    ...concerns are greater with SunTag. v1 SunTag exhibits some scaffold aggregation (v4 Suntag does not.) It’s up...may be toxic to the cell. Here comes the SunTag How does SunTag fix these problems? Instead of directly...power of SunTag for single molecule imaging, finding that plasma membrane-targeted CAAX-SunTag was 18-fold...traditional FP fusion, a SunTag fusion could be used as well. Pros of SunTag compared to traditional ...protein import into the nucleus. In developing their SunTag technology, the Vale and Weissman labs took this...signals. Named for the "stellar explosion SUperNova," SunTag can help you turn up the brightness in your fluorescent... interest, you instead fuse it to the synthetic SunTag scaffold. This scaffold contains 10-24 copies of...

  2. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

    Type
    Blog Post
    Published
    Oct. 6, 2020, 1:15 p.m.
    ...popular activation methods (VPR and SunTag) (Chavez et al., 2016). SunTag Description Rather than using a ...combines components of SAM and SunTag activators, fusing the epitope tag of SunTag with the P65-HSF activation...-engineer components of Cas systems themselves. SunTag, SAM, and VPR have all shown significant improvements...a single copy of VP64 per each dCas9, SunTag uses a repeating peptide array to fused with multiple copies...to be recruited per targeted gene. Performance SunTag performs better than first generation activators...system. Its activation levels are similar to that of SunTag. An advantage to this method compared to other ...dependent on gRNA design (SAM) or peptide design (SunTag). This streamlines its delivery, making it a common...

  3. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    Published
    Aug. 1, 2017, 1:15 p.m.
    ...contains 24 copies of SunTag, a synthetic scaffold that can recruit GFP fused to the SunTag-specific antibody...scFv. Each time the reporter is translated, the SunTag sites on the nascent polypeptide recruit scFV-GFP..., as the authors of these papers did. Since the SunTag-based system tethers mRNAs to the plasma membrane...Additional Resources the Addgene Blog Learn More about SunTag Catch up on Your GFP Background Choose the Brightest...

  4. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    Published
    June 3, 2021, 1:15 p.m.
    ... The SunTag system uses an scFv to amplify the fluorescence intensity of a tagged protein. SunTag has ...multiple copies of the scFv-GFP fusion bind the SunTag scaffold, the intensity of the fluorescent signal...Developmental Studies Hybridoma Bank Learn more about the SunTag system which uses an scFv to amplify the fluorescence...

  5. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    Published
    Aug. 11, 2015, 2:30 p.m.
    ...sensitivity of the technique. One potential solution is SunTag, a synthetic scaffold that can be used to recruit...from Addgene!) Since the signal level provided by SunTag is so high, cells may be imaged under lower illumination...

  6. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    Published
    July 2, 2019, 12:51 p.m.
    ...circumvented by the Yang Lab with the development of a Suntag-p65-HSF1 (SPH) transgenic mouse (Zhou et al., 2018...This mouse expresses a modified version of the “Suntag” Cas9 activator that recruits up to twenty copies...sequence. ES cells that stably express the dCas9-Suntag activator were transduced with a pooled sgRNA library...

  7. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ...inducible gene expression by creating the SunTag system. The SunTag system, named after the "stellar explosion...Gersbach CA, Nat Chem Biol 2015 Mar;11(3):198-200. SunTag system for single molecule imaging and inducible...to a target polypeptide. Multimerization in the SunTag system occurs via antibody-peptide labeling; specifically...intracellular processes. Multimerization via the SunTag system also shows strong upregulation of gene activation... activation when fused to dCas9. In the dCas9-SunTag-VP64 system, dCas9 is fused to a scaffold containing...transcription of the target gene. The brilliant SunTag system plasmids are available through Addgene and...

  8. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    Published
    Oct. 13, 2022, 1:45 p.m.
    ...VP64.  Later generations of activators include the SunTag system which facilitates recruitment of multiple...VP64, VP64 along with other SAM factors, and the Suntag array of factors, all targeted to the promoter ...

  9. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    Published
    Aug. 18, 2016, 2:30 p.m.
    ...We have found that SAM (Konermann et al., 2014), Suntag (Tanenbaum et al., 2014, Gilber et al, 2014), and...activators, check out our blog post covering VP64, Suntag, SAM, and VPR. Worry less about off targets Unlike...
Showing: 1 - 10 of 10 results