... sgRNA containing alternating MS2 and PP7 sequences (sgRNA-Sirius-4X(MS2-PP7)) that result in yellow fluorescence...) Diagram of sgRNA-Sirius-8XMS2, sgRNA-Sirius-8XPP7, and sgRNA-Sirius-4X(MS2-PP7) showing recruitment ...scaffolds that contain two hairpin sequences (either MS2, PP7, or boxB) that each recruits a different fluorescent...unstable.   Figure 1: CRISPRainbow labeling. Two MS2, PP7, or boxB sequences inserted into the sgRNA generates...green, or red colors for labeling. A combination of MS2, PP7, or boxB sequences in the same sgRNA will generate...’ end of the gRNA. Because of the success of the MS2 and PP7 RNA hairpin sequences in CRISPRainbow, they...for further development. They inserted an octet of MS2 hairpins into the gRNA tetraloop and went through...

...Blue MS2-MS2 Green PP7-PP7 Red boxB-boxB Cyan MS2-PP7 Yellow PP7-boxB Magenta boxB-MS2 White...White boxB-MS2-PP7 Despite the success of Ma et al.’s approach, fluorescently labeled Cas9’s come with...

...the 3’ UTR of the reporter mRNA via an MS2 coat protein (NLS-MS2-RFP) the reporter mRNA, which contains...achieved by using multiple copies of the PP7 and MS2 coat protein binding sites to tether many copies ...

...of these probes, fluorophore binding aptamers and MS2-binding fluorescent proteins were the state of the...as compared to imaging with broccoli aptamers or MS2 tags using the same number of fluorophores. You can...

...sgRNA incorporating two MS2 RNA aptamers at the tetraloop and stem-loop 2; 3. The MS2-P65-HSF1 plasmid which...

... engineered with aptamers that bind to MS2 proteins. These MS2 proteins then recruit additional activation...

...inactive Cas9-VP64, a sgRNA with two MS2 RNA aptamers, and the  MS2-P65-HSF1 helper protein (Konermann ...

...CRISPRa system in yeast is mediated by recruitment of MS2-functionalized gRNAs.  Find the yeast CRISPRa plasmids...

...the 3’ UTR of the reporter mRNA via an MS2 coat protein (NLS-MS2-RFP) the reporter mRNA, which contains... coat protein binding sites and 24 copies of the MS2 bacteriophage coat protein binding sites to tether... study to target various RNA molecules - such as MS2 stem loops, PPL stem loops, the Spinach2 aptamer,...

... by incorporation of an RNA hairpin, such as the MS2 hairpin, that is capable of recruiting additional...

...Figure 2). RNA production can be detected by using a MS2 coat protein that specifically detects a specific...

...transcription, but with two additional activation domains (MS2 and p65) to enhance transcriptional activation. Zhang...

...mammalian cells. This plasmid is used with pcDNA3.1-MS2-Tet1-CD to decrease methylation and activate transcription...

...to recruit an sgRNA with MS2 hairpin binding sites, which then recruit an MS2-fused hyperactive variant...

...Int J Mol Sci., 21(18): 6461 (2020). 10.3390/ijms21186461 Lin, S., Staahl, B. T., Alla, R. K., Doudna,...homology-dependent repair. Nat. Com., 9-1133 (2018). 10.3390/ijms21186461 Additional Resources on the Addgene Blog Check...

...Int J Mol Sci 24:5994. https://doi.org/10.3390/ijms24065994 Xu J, Xu K, Jung S, et al (2021) Nanobodies ...

...Mol Sci. 2023 Jan 13;24(2):1563. doi: 10.3390/ijms24021563 Resources on Addgene.org CRISPR Plasmids and...

...Fragments. IJMS 21:6324 . https://doi.org/10.3390/ijms21176324  Additional resources on the Addgene blog: ...

...Engineering. IJMS 22:5084. https://doi.org/10.3390/ijms22105084 Additional resources on the Addgene blog Learn...