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Showing: 1 - 20 of 29 results

  1. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    ... sgRNA containing alternating MS2 and PP7 sequences (sgRNA-Sirius-4X(MS2-PP7)) that result in yellow fluorescence...) Diagram of sgRNA-Sirius-8XMS2, sgRNA-Sirius-8XPP7, and sgRNA-Sirius-4X(MS2-PP7) showing recruitment ...scaffolds that contain two hairpin sequences (either MS2, PP7, or boxB) that each recruits a different fluorescent...unstable.   Figure 1: CRISPRainbow labeling. Two MS2, PP7, or boxB sequences inserted into the sgRNA generates...green, or red colors for labeling. A combination of MS2, PP7, or boxB sequences in the same sgRNA will generate...’ end of the gRNA. Because of the success of the MS2 and PP7 RNA hairpin sequences in CRISPRainbow, they...for further development. They inserted an octet of MS2 hairpins into the gRNA tetraloop and went through...

  2. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    ...Blue MS2-MS2 Green PP7-PP7 Red boxB-boxB Cyan MS2-PP7 Yellow PP7-boxB Magenta boxB-MS2 White...White boxB-MS2-PP7 Despite the success of Ma et al.’s approach, fluorescently labeled Cas9’s come with...

  3. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    ...the 3’ UTR of the reporter mRNA via an MS2 coat protein (NLS-MS2-RFP) the reporter mRNA, which contains...achieved by using multiple copies of the PP7 and MS2 coat protein binding sites to tether many copies ...

  8. Bacterial Expression Systems

    Type
    Collection
    ...been fused to the MS2 binding sites within this plasmid. pZE31-DsRed-ms2x2 26248 MS2 coat protein binding...RNA-binding protein, MS2. Can be used in combination with a fluorophore-tagged MS2 (such as that found ...RNA-binding protein MS2 fused to dsRED. Can be used in combination with a fluorophore-tagged MS2 (such as that...can be used in super resolution microscopy. pZE31ms2 25860 MS2 coat protein binding sites Philippe Cluzel...found in pZS*12-MS2-GFP ) to track RNA localization of your gene of interest once its appropriate cDNA has...that found in pZS*12-MS2-GFP ) to track RNA localization of your gene of interest once its appropriate ...appropriate cDNA has been fused to the MS2 binding sites within this plasmid. Return to Top Protein Purification...

  9. Lentiviral Prep Service

    Type
    Collection
    ...Zhang 61426 lenti MS2-P65-HSF1_Hygro MS2-P65-HSF1 Hygromycin lenti vector encoding the MS2-P65-HSF1 activator... complex with a 2A Hygro resistance marker (EF1a-MS2-p65-HSF1-2A-Hygro-WPRE) Zhang Pooled Barcoding Libraries...

  10. Zhang Lab CRISPR Page

    Type
    Collection
    ...sgRNA incorporating two MS2 RNA aptamers at the tetraloop and stemloop 2 The MS2-P65-HSF1 activation helper...Expresses the MS2-P65-HSF1 activator helper complex with 2A GFP 61424 : sgRNA cloning backbone with MS2 loops ...resistance marker 61426 : lentiviral vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro...marker 61427 : lentiviral sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-zeo resistance...

  12. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...the 3’ UTR of the reporter mRNA via an MS2 coat protein (NLS-MS2-RFP) the reporter mRNA, which contains... coat protein binding sites and 24 copies of the MS2 bacteriophage coat protein binding sites to tether... study to target various RNA molecules - such as MS2 stem loops, PPL stem loops, the Spinach2 aptamer,...

  14. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...Mammalian BbsI yes, cut S. pyogenes Puro Zhang sgRNA(MS2) cloning backbone 61424 Mammalian BbsI none S. pyogenes...Lentiviral BsmBI none S. pyogenes Puro Zhang lenti sgRNA(MS2)_zeo backbone 61427 Mammalian/Lentiviral BsmBI none...Lentiviral hU6 none S. pyogenes Puro Moffat lenti sgRNA(MS2)_puro backbone 73795 Mammalian/Lentiviral U6 none...yes, interfere S. pyogenes URA3 Davis lenti sgRNA(MS2)_puro optimized backbone 73797 Mammalian/Lentiviral..., cut (enhanced) S. pyogenes Puro Abbosh pCFD3-MS2tail-Scaffold 78906 Fly dU6:3 none S. pyogenes Virmilion...

  19. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...to recruit an sgRNA with MS2 hairpin binding sites, which then recruit an MS2-fused hyperactive variant...

  20. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Jackson 78624 sgRNA(MS2)_Prnp_SAM1 PRNP U6 Dementia Walker Jackson 78625 sgRNA(MS2)_Prnp_SAM2 PRNP U6 ...148112 pcDNA3.1-MS2-HA-HshnRNPA1_Z HNRNPA1 CMV ALS Elisa Izaurralde 148113 pcDNA3.1-MS2-HA-HshnRNPA2-B1...Alzheimer's Axel Ullrich 65812 pCI-MS2V5-eIF4G delN83 EIF4G1 V5, MS2 CMV Parkinson's Niels Gehring 66818... GFP CMV Parkinson's Elisa Izaurralde 148960 pT7-MS2-HA-C1-HsGIGYF2_1-718_AI GIGYF2 HA CMV Parkinson's...
Showing: 1 - 20 of 29 results