...large genome edits using transposases or the Cascade-Cas3 system. We also describe newly engineered Cas9s...

...systems, however, Cascade from VchINT does not recruit other nucleases (such as Cas3) or have its own ...requirement for multiple Cas proteins to form the Cascade targeting complex is a hallmark of Type I CRISPR-Cas...proteins (TniQ, Cas8, Cas7, Cas6) that forms the QCascade DNA-targeting module together with the crRNA.  ... upstream 2-bp PAM sequence for recognition by QCascade; although 5’-CC-3’ is one of the best PAMs for...recognized by the system. Guided by the crRNA, QCascade complexes survey the genome and bind target DNA...downstream of the genomic target site bound by QCascade (Fig. 1B). The process also creates a 5-bp target-site...

...that interact with CRISPR-Cas systems such as Cascade-Cas3, Cas9, Cas12, and Cas13 (Marino et al., 2020...

...from gut. While still far from the clinic, CRISPR-Cas3 systems have been used successfully in mice for ...Clostridioides difficile Using Phage-Delivered CRISPR-Cas3 Antimicrobials. mBio 11: . https://doi.org/10.1128...1) a CRISPR RNA, (2) four proteins forming the QCascade DNA-targeting module with the crRNA, (3) three...