Addgene

We narrowed to 219 results for: pET

Showing: 161 - 180 of 219 results

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Adenoviral Vector Production and Troubleshooting

Type

Blog Post

Published

June 14, 2018, 11:44 a.m.

...response against AdV. Importantly, recombination-competent adenovirus (RCA) can be generated if the E1 gene...
Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

Type

Blog Post

Published

Dec. 14, 2021, 2:15 p.m.

...Charlton J, Jung EJ, Mattei AL et al. (2020) TETs compete with DNMT3 activity in pluripotent cells at thousands...
Meet an Addgenie: Insights into Grad School, Career Paths, and More

Type

Blog Post

Published

June 25, 2020, 1:15 p.m.

...discovered that science here is more stressful and competitive than back home. Scientists are often under a ...
Cpf1 Update: Comparison to Cas9 and NgAgo

Type

Blog Post

Published

July 14, 2016, 2:30 p.m.

... Up On Your Cpf1 Background Read About CRISPR Competitor NgAgo Learn How You Can Use piggyBac Vectors ...
Plasmids 101: Terminators and PolyA signals

Type

Blog Post

Published

March 31, 2016, 2:30 p.m.

... weaker poly(A) signals like BGH. References Peters JM, Vangeloff AD, Landick R. Bacterial Transcription...
Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

Type

Blog Post

Published

Dec. 1, 2015, 3:30 p.m.

...pathways are involved in RNA based surveillance of repetitive gene families and provide a way to guide inheritance...
Gaining Leadership Skills Volunteering at a Professional Organization

Type

Blog Post

Published

Aug. 6, 2015, 2:30 p.m.

...Postdoctoral Association (NPA) outlines 6 Core Competencies that all postdocs (but also PhDs) should develop...
Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

Type

Blog Post

Published

June 5, 2014, 3:32 p.m.

...attend conferences makes it very challenging to compete in today’s tough academic or industry job market...
PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

Type

Blog Post

Published

June 25, 2024, 1:15 p.m.

.... M., Pallaseni, A., Kuzmin, I., Raudvere, U., Peterson, H., Liberante, F. G., & Parts, L. (2023). Prediction...
Tips for a 1st time AAV user (by a Rookie AAV user)

Type

Blog Post

Published

Oct. 23, 2018, 11:49 a.m.

...bacterial strains, like NEB Stable. NEB Stable competent cells lack RecA, a protein that aids in the homologous...
PCR Applications for Copy Number Variation Assays

Type

Blog Post

Published

April 26, 2022, 1:15 p.m.

... RQ value of 1.5 means you have either made a pipetting error or an incredible discovery. The range of...
Google Forums Round Up: First Impressions of NgAgo

Type

Blog Post

Published

Aug. 4, 2016, 2:30 p.m.

...blogs, or in the comments section below. Keep pipetting and happy genome editing! Check Out Other Argonaute...
DNA Purification Without a Kit

Type

Blog Post

Published

Feb. 11, 2020, 2:15 p.m.

...mammalian cells in 30 seconds or less without using a pipette. Here’s how this method works: nucleic acids are...
A Guide to Starting Your Own Journal Club

Type

Blog Post

Published

Feb. 6, 2020, 3 p.m.

...literature and emerging technologies to remain competitive and relevant. Enter: the journal club. A journal...
MXS Chaining

Type

Blog Post

Published

Feb. 7, 2017, 3:30 p.m.

...for the modular assembly of highly similar or repetitive sequences. Intermediate chaining products (called...
Course-Based Undergraduate Research Experiences (CUREs)

Type

Blog Post

Published

Feb. 9, 2023, 2:15 p.m.

...Einstein Discovers America. New York./ Callahan, K., Peterson, C., et al. 2022. External collaboration results...
CRISPR 101: Making a Knock-In Cell Line

Type

Blog Post

Published

Dec. 22, 2022, 2:15 p.m.

... al., Nat Com). Fig. 3: HDR events compete with NHEJ-mediated edits of small insertions and...
Summer SciComm Series: Modes of Communication

Type

Blog Post

Published

July 1, 2022, 1:15 p.m.

... needed. Traditional media formats are quite competitive while digital can be oversaturated. Transition...
Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

Type

Blog Post

Published

Aug. 27, 2020, 1:15 p.m.

...that inhibit BiFC in your cellular system. BiFC competition analysis can be used in lieu of negative controls...
CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

Type

Blog Post

Published

Aug. 9, 2016, 2:30 p.m.

...been full of dark matter: enhancers, lncRNAs, repetitive elements, repressors, insulators, and more. We...

Showing: 161 - 180 of 219 results

Results per page:

Adenoviral Vector Production and Troubleshooting

Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

Meet an Addgenie: Insights into Grad School, Career Paths, and More

Cpf1 Update: Comparison to Cas9 and NgAgo

Plasmids 101: Terminators and PolyA signals

Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

Gaining Leadership Skills Volunteering at a Professional Organization

Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

Tips for a 1st time AAV user (by a Rookie AAV user)

PCR Applications for Copy Number Variation Assays

Google Forums Round Up: First Impressions of NgAgo

DNA Purification Without a Kit

A Guide to Starting Your Own Journal Club

MXS Chaining

Course-Based Undergraduate Research Experiences (CUREs)

CRISPR 101: Making a Knock-In Cell Line

Summer SciComm Series: Modes of Communication

Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

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