...for imaging a protein’s entire life-cycle. This makes it nearly impossible for scientists to use GFP to...large, dim, and permanently attached to a protein, making them hard to amplify and image for long periods...in vivo. These probes however can be expensive to make, hard to deliver into living systems, and often ... proven to fold and function in living cells. To make these scaffolds specific to different targets, the...

...you believe your idea has potential to make a difference, then make sure you’re able to answer every possible... Start with your network You've spent your life making connections and finding mentors -- well, it's time...discuss each project and business decision before making it, to have an internal review system.  On top ...

...method limits screening to a few hundred worms, making the detection of rare events difficult. Dickinson...resistance and the Roller movement phenotype. To make these markers easy to remove after injection, they... to generate a self-excising cassette (SEC). To make it possible fluorescently label proteins in C. elegans...fluorescent protein fusions, this system could be used to make many other genome modifications, including targeted...

..., as it can edit near to or far from PAM sites, making it less constrained by PAM availability than other...within the base editing window, while prime editors make a specific edit defined by the pegRNA. In cases ...the non-edited strand, they can cause the cell to remake that strand using the edited strand as the template... These variants are worth testing if you want to make your prime editing as efficient as possible. ...Some examples include: TwinPE: two prime editors make complementary edits to opposite strands of the DNA...

...generating/replenishing kits, is to find new ways to make lab processes more efficient. Being in the Boston...there because both LabCentral and Addgene aim to make scientific advancement easier. NEBNext Ultra FS ...we did at the workshop (the kit is mostly used to make new libraries as opposed to replicating them like...new without spending a lot of time developing and making space for the techniques ourselves. As you probably...

...are able to make when they have access to shared materials, and then pay it forward by making the new materials...has been able to rapidly propagate this strain, making it available to scientists around the world. This...research by removing logistic hurdles to continue making materials available rapidly to scientists (Nature...

...pombe (fission) and S. cerevisiae (budding). Yeast make for an excellent model organism for a few reasons...phaffii is highly efficient at protein secretion, making harvesting recombinant proteins a cinch. This is...it doesn’t secrete its own endogenous proteins, making the recombinant protein its major secretion. Beyond... removing methanol from the media. This feature makes the strain tractable for peroxisomal studies on ...

... strategies can make socially distanced work more creative. An example could be making lab meetings more...without leaving Zoom. The annotate function can also make the screen-sharing experience more interactive. ...: “Science is increasingly done in large teams, making it more likely that papers will be written by several...

...Christopher Voigt and Jeffrey Tabor have been working on making E. coli light responsive since 2005, when Tabor...systems continue to be optimized by Tabor’s lab, making light-controlled gene expression in E. coli easier...the response regulator encoded in the genome. To make the system less bulky and easier to use with other...Schmidl et al. chose to optimize these systems to make them more tunable and user-friendly. They experimented...

...the earliest) or a functional one. Pick one that makes sense for your skills, and the job you are applying...seconds on a resume when they first look at it. Don’t make them have to guess what you are capable of. You ...might know a thing or two about computer modeling. Make sure to focus on these skills. The only exception... yet for that dream job. Also, many universities make their job postings and salary expectations public...

... digest tips and tricks: The following tips will make it easier for you to obtain a useful and informative... recognition sequence. Most enzyme manufacturers make High Fidelity versions of the endonucleases and/...before loading them. The glycerol in the buffer will make sure your sample settles in the gel well and the...wrong! Quantify your DNA. Loading too much DNA will make it difficult to obtain crisp bands and analyze the...

...efficiency. Check out Addgene’s blog to learn about making your own competent cells and our protocols page... encodes a green fluorescent protein gene which makes the bacterium fluoresce green. Comic by Maya Kostman...Lytic phages hijack the bacterial hosts machinery to make more viral particles. Eventually the cell lyses ...bacterial chromosome and allows the donor bacterium to make a small “bridge” or sex pilus that attaches to the...

...feature, you can compare different Cas enzymes to make sure you are getting the desired features and cutting...and discover orthologs with similar properties (Makarova et al). CasPEDIA is an evolving database, and... to it! The CasPEDIA Consortium will, of course, make efforts to keep it updated with new entries and ...Structural Biology. https://doi.org/10.32942/X2C31F     Makarova, K. S., Wolf, Y. I., Iranzo, J., et al. (2019)...

...think. So, if you’ve ever wondered what goes into ‘making virus’ or what gag and pol stand for, we demystify...recombinant adenovirus is missing the endogenous E1 gene, making it replication deficient. This is typically supplied... some of the plasmids in our collection. Want to make a vector construct on your own? No problem, we are...discussed are compiled for reference below. Happy virus making!  References and Resources Kulanayake, S., Tikoo...

...value. Types of biosensors So now that we know what makes up a biosensor, we can discuss what flavors of sensors...difficult to achieve in whole organisms, but AAVs make it possible. In AAV systems, different serotypes...expression and downstream monitoring. Nervous about making a virus for your biosensor? Don’t be! We have you...viral resources and safety tips. Still worried? We make virus with some of our most popular biosensors already...

...summaries of recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you...Parton Lab recently created a flexible system that makes use of pre-existing transgenic lines, termed BLITZ...system provides researchers better flexibility and makes the approach more accessible, as it reduces the ... be packaged into an AAV system. dCas9-SSAP will make the editing of kilobase-scaled sequences more efficient...

... explore small-scale opportunities where I could make mistakes and learn from them. I started at my previous...Beyond Academia: Stories and Strategies for PhDs Making the Leap to Industry." He successfully transitioned...Resources  Resources for aspiring managers Books: The Making of a Manager by Julie Zhuo Radical Candor by Kim...Managing vs. Leading Management for Scientists: What Makes a Good Leader Anyways?  Transferable Skills Guide...

...character you painted in your personal statement. Make certain to choose your recommenders based on your...individuals are inundated with requests. The easier you make it for them, the likelier you will end up with a...lighting for a smooth performance. Most importantly, make certain that everyone you live with is aware of ...consistent with your personal statement, while you make the ultimate case: why you? 7. Strength in numbers...

...variety of educational resources in an effort to make scientific knowledge broadly available.  The ability... return the matching resources in an order that makes sense to the user. The devil is in the details of... of course: what does “best” mean? and what “makes sense” to a user? Moreover, there are many ways a user...institutions. There are many more technical details that make our new search engine unique. Most importantly, ...

...will be a positive asset throughout your career? 1. Make scicomm practice a priority The good news for young...of the work you presented may also be unclear. 2. Make the audience your primary focus When writing a paper...address that 'why' question effectively, you need to make your audience the focus of every sentence you write...know this? How will they react to it - what will it make them think? Does this lead to the conclusions I ...