We narrowed to 530 results for: arl
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TypeBlog PostPublishedMarch 9, 2017, 3:40 p.m....better, no grant writing Job changes can happen regularly (every 4-6 years is common), but this can also...diverse options and how to take advantage of them early is one way to smooth your path to a fulfilling career...
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In Vivo Biotinylation of Bacterial Fusion Proteins
TypeBlog PostPublishedJan. 25, 2018, 2:09 p.m....plasmid in NEB T7 SHuffle bacteria (C3029J). We are clearly NEB “fanboys” as the bacteria significantly enhance...biochemist in the Vanderbilt Eye Institute and is particularly interested in cell signaling. Additional... -
Identifying Sequence Elements with SnapGene's Feature Database
TypeBlog PostPublishedDec. 21, 2017, 2:06 p.m....commercial plasmids were inconsistent and sometimes clearly wrong. In an effort to be rigorous, we dug into...Biology at the University of Chicago. He was an early depositor at Addgene, and conceived SnapGene because... -
Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants
TypeBlog PostPublishedJune 22, 2021, 1:15 p.m....Alpha variant), B1.1.298, and B.1.429 (Epsilon), nearly as well as they could for the wild-type. However...TM, Clayton KL, Nitido AD, Murali MR, Alter G, Charles RC, Dighe A, Branda JA, Lennerz JK, Lingwood D,... -
What the HEK?
TypeBlog PostPublishedApril 18, 2023, 1:15 p.m....two areas of research where HEK cells may be particularly useful due to their intrinsic properties. ...by mycoplasma and bacteria, but HEKs are also particularly vulnerable to viral infection. Yes, your cells... -
New and Upcoming Viral Vectors - December 2019
TypeBlog PostPublishedDec. 19, 2019, 2:40 p.m....We have also packaged hChR2(H134R) plasmids from Karl Deisseroth’s lab, which includes pAAV-hSyn Con/Fon...WPRE-SV40 124650 AAV9 pAAV-CamKIIa-C1V1(t/t)-mScarlet-KV2.1 55645 AAV5 pAAV-hSyn Con/Fon hChR2(H134R... -
Donations from Addgene to Yield Answers for Rare Disease Researchers
TypeBlog PostPublishedSept. 15, 2015, 2:30 p.m....by mutations in the OCRL1 gene that leads to the early death of young patients as their kidneys fail. The...interested in XP out of a desire to understand the early events in the transformation of healthy cells into... -
Celebrate National Book Month with Addgene
TypeBlog PostPublishedOct. 3, 2019, 12:47 p.m....insects, mammals, and plants, among others. I particularly enjoyed the emphasis that microbes are neither...last 8 years. Since then, we’ve read books across nearly every genre you can imagine: gothic, mystery, sci-fi... -
Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System
TypeBlog PostPublishedJune 4, 2019, 1:02 p.m.... 2017. These travel awards are intended to help early career scientists attend a conference of their choice...evidence suggests that it is incredibly important for nearly every aspect of life.” Unger began with the a bacterial... -
Creating accessible biology activities in schools with BioBits
TypeBlog PostPublishedAug. 2, 2018, 12:56 p.m....thinking: I wish I had this kind of experience earlier! After all, students build circuits in physics... about operating the rest of the car as well. Similarly, cell free expression systems contain all the ... -
Plasmids 101: Gram Negative and Positive Bacteria
TypeBlog PostPublishedAug. 2, 2022, 1:15 p.m....decolorized as gram negative. A German scientist, Carl Weigert, later expanded on Gram’s discovery, adding...resources. Bruckner, M. Gram Staining. https://serc.carleton.edu/microbelife/research_methods/microscopy/gramstain.html... -
Using CRISPR/Cas9 to Edit Disease Out of the Genome
TypeBlog PostPublishedJune 23, 2022, 1:15 p.m....They are targeting the genes responsible for both early onset and late onset AD. In this study, the group...autosomal-dominant mutations that have been connected to the early onset of Alzheimer’s disease, as well as those that... -
The MTA at Addgene
TypeBlog PostPublishedJuly 23, 2024, 2:17 p.m....MTAs by the Provider and Recipient (in Addgene parlance, the depositor and requestor) organizations. Over...depends on things being organized, tracked, and clearly communicated. And that’s where MTAs, and similar... -
Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond
TypeBlog PostPublishedJune 1, 2021, 1:15 p.m.... neutralize the SARS-CoV-2 virus. But while the early results were promising, the researchers did not ... the preprint repository BioRxiv. This example clearly illustrates the power of collaboration, which despite... -
Addgene Welcomes John Doench to the Advisory Board
TypeBlog PostPublishedAug. 13, 2015, 2:30 p.m....specificity in the miRNA regulatory process. This early research is still relevant to his work at the Broad...screens to learn more about cancer biology in Ed Harlow’s group at Harvard Medical School and then applied... -
Behind the scenes: Addgene’s new search engine and more
TypeBlog PostPublishedDec. 12, 2019, 2:22 p.m....results. The footer and improved menus we launched earlier in the year, as well as our redesigned homepage...categorization of our information in order to more granularly customize the search engine, and a scoping mechanism... -
Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME
TypeBlog PostPublishedJune 6, 2019, 12:41 p.m....miRNA reporter podcast segment Discovered in the early 1990’s, microRNAs (miRNAs) are recognized as important...analysis of immune cells has been a gap in the field. Arlene Sharpe’s group at the Harvard Medical School developed... -
Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies
TypeBlog PostPublishedJuly 28, 2017, 1:30 p.m....research (3). The sky is the limit. As mentioned earlier, even genes not found in Drosophila can be studied...immunology PhD at Harvard University. He has had nearly a decade of experience working with Drosophila ... -
Antibodies 101: Isotypes
TypeBlog PostPublishedOct. 19, 2021, 1:15 p.m.... in Health and Disease. 5th edition. New York: Garland Science; 2001. Structural variation in immunoglobulin... in Health and Disease. 5th edition. New York: Garland Science; 2001. The distribution and functions of... -
Viral Vectors 101: Inverted Terminal Repeats
TypeBlog PostPublishedOct. 19, 2023, 1:15 p.m....because they demonstrate high promoter activity (Earley et al. 2020), but non-AAV2 ITRs can be used to ...microorganisms1202031038399714. PMID: 38399714. Earley, L. F., Conatser, L. M., Lue, V. M., Dobbins, A...