...and respect your IP rights and data ownership? IP and data ownership should be yours (your institution’...sure you don’t use ELN providers that take any ownership over your data or have direct access to it. Your...

...non-functional, you may simply introduce a frame-shift mutation. This can be achieved using a restriction...standard ligation reaction resulting in a frame-shifted coding region. It is worth noting that unlike these...specifically designed mutations (not limited to frame shift mutations). Consequently, site directed mutagenesis...

...GFP to be translated), and second, through a gel shift assay using the PhosTag system (see below). This...phosphate moieties and promotes a robust mobility shift in phosphorylated, but not non-phosphorylated proteins...done using mass spectrometry to identify a mass shift proportional to a single phosphate moiety on the...

...Karnan, S., Ota, A., Hossain, E., Konishi, Y., Hosokawa, Y., & Konishi, H. (2014). A comparative analysis...j.omtn.2017.03.009 Watakabe, A., Ohtsuka, M., Kinoshita, M., Takaji, M., Isa, K., Mizukami, H., Ozawa,...

...genetics.120.303564 Kim H, Ishidate T, Ghanta KS, Seth M, Conte D Jr, Shirayama M, Mello CC (2014) A Co-... . https://doi.org/10.1534/genetics.115.178335 Dokshin GA, Ghanta KS, Piscopo KM, Mello CC (2018) Robust...

...recombination genes at low temperatures (30-34˚C).  Shifting bacteria to 42˚C for 15 minutes quickly inactivates... is not required for maintenance. A temperature shift to 42˚C not only allows for activation of the red...Department of Laboratory Medicine at the University of Washington. She is particularly interested in science communication...

...remotely for the last year to give our lab and shipping teams enough space at HQ and for some of us, that...email from us asking for your mailing address and T shirt size. You have until April 7, 2021 to make your...

...PMC4269290. Masamizu, Y., Okada, T., Kawasaki, K., Ishibashi, H., Yuasa, S., Takeda, S., Hasegawa, I., and ...PubMed PMID: 1381034. Watakabe, A., Ohtsuka, M., Kinoshita, M., Takaj, M.I., Isa, K., Mizukami, H., Ozawa...

...countries. Just eight days into 2024, we've already shipped 2,078 items to 764 scientists at 375 organizations...different countries. It's safe to say we keep our shipping team busy! But they're not alone: to better serve...

... cannot be washed or reused/repurposed in some fashion. Addgenies swapping out printer cartridges can ... striving to set an example of environmental stewardship.  Additional resources on the Addgene blog ...

...of busy Addgenies preparing plasmid samples for shipping. This was the favorite part of their visit for...sure to tell us what we already knew about our Shipping Operations Coordinator: “Kory was awesome!”. The...

...To-Do list long enough that there is no hope of accomplishing it all. How do we encourage people to take a... Hack Days, and we’ve seen great success at accomplishing “we-should-really-make-time-for-that” type projects...

... pizza or pub.” The Cepko Lab celebrates any fellowships, jobs, or paper submissions with dinner and champagne...sharing meals, going on trips, and going to New Hampshire in the summer for the annual lab retreat. How ...

...mentors and mentees have fulfilling, productive relationships. I will be sharing what I’ve learned along the...many had examples of very productive mentoring relationships with advisors and others who helped shape their...

... See highlights from the COVID-19 collection Partnership with Ginkgo Bioworks  While one way to help the... both academic and industry scientists. This partnership complemented each other's strengths: Ginkgo Bioworks...

...few months, the scientific community has seen a shift towards prioritizing research into this disease....policies and ultimately treatments in a timely fashion.”  With this in mind, Bourque and Tara Moriarty...

...release in keeping with their value of shared ownership. Go check it out! References and Resources References... Speranza, E., Chu, C. J., Gola, A., Thakur, N., Shih, R., Yao, L., Yaniv, Z. R., Beuschel, R. T., Kabat...

...you have any particular tips or tricks for those wishing to purchase and use the SpyLigase system for the...others to Addgene. I am a big fan; thanks for the T-shirt! References: SpyLigase peptide-peptide ligation...

...genome-wide CRISPR knockout library from Xiaole Shirley Liu's lab targets all mouse genes with 10 gRNAs...of ready-to-use AAV9 and AAVrg vectors. New red-shifted INTRSECT 2.0 calcium sensor available in AAV8! ...

...better with some variety in their job. Good relationships with clients and colleagues Sometimes this means...scientists) Are self-directed, but need precise leadership and support from their superiors (beware of not...