...agencies to propose reforms to the H-1B visa system. Large firms and firms that work on projects with an international...

...transfected with low efficacy. But since there can be large differences between endogenous expression and transfected... This can be particularly useful when designing larger multicolour panels, where different manufacturers...

...distributing widely throughout the organism to target large or diffuse biological structures. Of primary interest...transduction in Old World monkeys AAV.CAP-Mac is largely neurotropic in infant rhesus macaques and green...AAVs to transduce the same cell, can accommodate larger, modular genetic payloads. Since most systemic ...

...redshifted-emission spectrum compared to that of CyOFP1, a large stokes shift, long fluorescence lifetime, and a ...imaging. These variants were developed as part of a larger strategy to engineer improved red fluorescent proteins...

...electrophoresis. Smaller fragments migrate quicker than larger fragments. These fragments are quantified over ...that smaller fragments are quantified first before larger fragments. Figure 1: The quantification of...time. Smaller fragments migrate more quickly than larger fragments. Image from OpenWetWare. This method...

...with a transfectable cell line. 2) They require larger expression vectors which are often more difficult...into viral vectors. Cas9 multiplexing vectors are larger because they require regulatory sequences to allow...individual promoters).  spCas9 and its gRNAs are also larger than their Cpf1 counterparts. Table 2. Cas9 Multiplexing...

...outlier, this story had us wondering: is there a larger trend here? Do papers associated with Addgene deposits...deposits are more highly cited, we went to one of the largest bibliographic databases available, Web of Science...

... and mediate biochemical processes. One of the largest and best characterized of these is the Src homology... sequencing, revealing that they bind to a much larger subset of protein motifs than previously thought...

...preps. This new Viral Service represents Addgene’s largest new initiative since we started distributing plasmids...support we are able to provide faster access to a larger number of viral preps. Visit our Sponsors and Supporters...

...hundred bps of sequence. However, if you have a larger knock-in (200 bp – 2 kb), a dsDNA donor will need...oligonucleotides. These donors traditionally have larger homology arms in the 500 bp to 1 kb range, however...

...accomplishment while the longer tasks will give you larger payoffs later. Don’t be afraid to accomplish some...ve also recently started using Trello to manage larger, multi-person projects. Day-to-day, flexible to-do...

...consequence, the preparations for this technique must be larger scale, to allow the extraction of enough plasmid...

...Addgene colleagues, collaborators, partners, and the larger scientific community to maximize the positive impact...

... Cas14 could be a more primitive version of the larger and more complex Cas9 and Cas12 proteins. Cas14...

...event and to expand the conference to accommodate a larger number of attendees. This first-year event’s success...

...creations can go on display for all to enjoy. The largest competition of this kind is Princeton University...

...vectors. At 3500 plasmids, this represents the largest single deposit in our repository and was a three–year...

...What is the advantage of peptide tags for building larger protein assemblies? Howarth: Peptide tags can be...

... but in some biological systems one “might need larger numbers.” Further details and protocols are available...

...like lab meeting presentations. You can download larger images without the watermark, which are better ...

...over hosting them. Since then, webinars make up a larger part of Hans’ workload. “I am currently getting...

...to try out techniques that were discussed in the larger sessions and reinforced our newfound knowledge ...

...different however. In industry the career ladder is larger and there are more opportunities for promotion....

...wanted to share these, and other, findings with the larger content and marketing team, and I started preparing...

... and two-photon excitation around 1,040 nm, has larger photocurrents, and is more selective for K+ than...

...cleavage efficiency. If this finding is replicated in larger studies, it will represent a key advantage over...

...Relevant, and Time-bound. It is a good idea to take larger goals (e.g. Figure out what I want to do after ...

...78% in body wall muscle cells. These changes were larger than previously reported for other organisms, and...

... "The Mistborn series is the first 3 books of a larger epic of fantasy novels. Set in a world where 1000...

...the cell, and potentially spread out over an even larger area, thus necessitating even higher retrograde...

...screening experiments, so the scale of the DNA prep is larger. And since the library contains many plasmids, ...

...are being given in vivo, it is difficult to use larger enzymes. To reduce the impact of off-target mutations...

...TagRFP excitation and the emission filter collects a larger fraction of the TagRFP emission than the mCherry...

...the gel for longer to achieve good separation of larger DNA fragments. I hope these tips demonstrate ...

... making this choice.  How big is the lab? While larger labs tend to be more "chaotic" and offer less micromanaging...

...pushed the field further with the creation of the largest miRNA sensor collection reported to date, containing...

...reagents with the scientific community.   (click to enlarge) As we look ahead to the things to come, we wanted...

...Elasticsearch to support the different usage patterns the larger team identified. The basic function of a search...

...www.wannapik.com/   Even though ChIP requires a larger upfront investment than many other antibody applications...

...activate or inhibit neuronal firing. As GPCRs are the largest class of signal transducing receptors in the brain...