...individual plasmid within a single bacterial cell. Large plasmids usually have a low copy number (approximately... other hand, smaller plasmids can be present in large numbers, 50 or more per cell and have a high copy...usually want to start 2 mL in a falcon tube, but for larger preps you might want to use as much as a liter ...

... from 10-50 µL depending on application and is largely determined by the volume of DNA to be cut. A typical...website about star activity . If you are digesting a large number of plasmids with the same enzyme(s) (for ...

...less efficient at taking up larger plasmids. If you need to transform large plasmids, it is a good idea...very few if any transformants when transforming large plasmids (>10 kb) or BACs, what can I do? Chemically...

...30 °C instead of 37 °C. This is often true for large unstable plasmids, which sometimes recombine at ...

...occurring bacterial endonucleases that recognize a large range of DNA sequences. Given the variety of these...

...immediately, and decontaminate as necessary. For large spills that cannot be easily cleaned, evacuate and...

... liquid. Pro-Tip If pipetting from a relatively large container holding a small volume of liquid, tilt...

...Additionally, if your final product is going to be very large (>10kb) you might want to use electro-competent ...

...replication use a HEK293 line stably expressing the SV40 large T antigen. For antibody-expressing plasmids containing...

...Additionally, if your final product is going to be very large (>10kb) you might want to use electro-competent ...

...should be very little background staining. Pro-Tip A large shift in the electrophoretic mobility of the bands...

... you will be screening a large number of colonies, we recommend using larger plates. Many labs use 100...

...efficient means to scale-up without having to handle a large number of T-175 flasks. Workflow Timeline Day 0:... (PES) filter system, Nalgene, 565-0010 (or for larger volumes 1000 mL 0.45 µm Rapid-Flow PES Filtration...

...microfuge tubes used in labs, though some larger models can hold larger conical tubes. Depending on the centrifuge...speeds to their smaller cousins, but can hold much larger containers. Ultracentrifuges are also generally...generally floor sized centrifuges that can hold larger containers, but they can also rotate at much higher speeds...

...are magnification (the ability to make an image larger) and resolution (the ability to distinguish between...diaphragm and use the coarse focus adjustment knob (the larger of the two focus knobs) until you can see the edges...magnification, you will notice that the objectives get larger. Be careful to avoid hitting your sample with the...magnification, remember that the objectives are larger. Adjust your focus slowly so that you don't accidentally...

..., and a lower percentage gel will help separate larger bands. 10% Rule For each sample you want to load...

...places. Pro-Tip If you’re weighing out an amount larger than the capacity of your balance or weighing boat...

...proportionally. 1μL of ligase should be sufficient for larger ligation reactions. Because ligase buffer contains...

..., and a lower percentage gel will help separate larger bands. 10% Rule For each sample you want to load...

... of bacteria to grow (low copy plasmids require larger cultures). Centrifuge the culture to pellet the...