Addgene

We narrowed to 218 results for: pET

Showing: 141 - 160 of 218 results

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Creating accessible biology activities in schools with BioBits

Type

Blog Post

Published

Aug. 2, 2018, 12:56 p.m.

...skills like developing a hypothesis, precisions pipetting, and good record keeping. Finally, if you are ...
New and Upcoming Viral Vectors - May 2020

Type

Blog Post

Published

May 28, 2020, 1:15 p.m.

... Maya Peters Kostman...
Developing a cold tolerant E. coli using specialized chaperones

Type

Blog Post

Published

Jan. 28, 2020, 2:35 p.m.

... Medal in the Undergrad category at the iGEM Competition 2019. He has an active interest in scientific...
GCE4All: Making Genetic Code Expansion Accessible

Type

Blog Post

Published

June 15, 2023, 1:15 p.m.

...biology scene in 1989 through a seminal paper from Peter Schultz’s lab describing a technique that allowed...
Viral Vectors 101: Viruses as Biological Tools

Type

Blog Post

Published

March 21, 2023, 1:15 p.m.

...nature, extra safety measures, like replication incompetent viral particles, are required. We will be covering...
Viral Vectors 101: Gamma-Retroviral Packaging Systems

Type

Blog Post

Published

Aug. 6, 2024, 1:15 p.m.

...interchangeably. Are γ-retroviruses replication incompetent? Yes, as with lentiviruses, the genes required...
Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

Type

Blog Post

Published

Feb. 18, 2014, 4:22 p.m.

... and help each other practice meeting people – compete for fastest to set up a coffee date or most cards...
CRISPRainbow and Genome Visualization

Type

Blog Post

Published

Feb. 28, 2017, 3:30 p.m.

...(GAL4) was supplied. In addition to labeling repetitive telomeric sequences, Chen et al. successfully...
Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

Type

Blog Post

Published

Aug. 5, 2021, 12:15 p.m.

...used along other thiol-containing acceptors in competition assays. “I believe that improved biosensors or...
To Each HIS Own

Type

Blog Post

Published

March 2, 2023, 2:15 p.m.

...then eluted using a high imidazole buffer that outcompetes the interaction between the protein of interest...
Summer SciComm: Preprints

Type

Blog Post

Published

Aug. 16, 2022, 1:15 p.m.

...//doi.org/10.1242/bio.059310 Fraser N, Mayr P, Peters I (2021) Motivations, concerns and selection biases...
Addgene's Tips for Plasmid Quality Control

Type

Blog Post

Published

Jan. 14, 2016, 3:30 p.m.

...primers that bind within or must read through repetitive regions (such as LTRs or ITRs), hairpins, or ...
5 Steps to Writing Grant Proposals for Grad Students

Type

Blog Post

Published

Feb. 11, 2014, 12:13 p.m.

...assume that their advisor alone applies for these competitive grant applications; however, in some cases, your...
Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

Type

Blog Post

Published

March 8, 2017, 3:15 p.m.

...cloning strain DH5α. For plasmids with highly repetitive sequences (prone to recombination in bacteria...
Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

Type

Blog Post

Published

Dec. 14, 2021, 2:15 p.m.

...Charlton J, Jung EJ, Mattei AL et al. (2020) TETs compete with DNMT3 activity in pluripotent cells at thousands...
A Look Back at One Year of Plasmid Sharing for COVID-19 Research

Type

Blog Post

Published

July 12, 2021, 1:15 p.m.

... CR, Chu HY, Tortorici MA, Veesler D, Murphy M, Pettie D, King NP, Balazs AB, Bloom JD (2020) Protocol...
Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

Type

Blog Post

Published

May 4, 2021, 1:15 p.m.

...validation experiments performed will be useful in perpetuity. Second, plasmids are easy to store and share...
Hot Plasmids and Viral Preps - January 2021

Type

Blog Post

Published

Jan. 5, 2021, 2:15 p.m.

...cells. Casɸ from Biggiephage can eliminate competing mobile genetic elements. Image by Basem Al-Shayeb...
Meet an Addgenie: Insights into Grad School, Career Paths, and More

Type

Blog Post

Published

June 25, 2020, 1:15 p.m.

...discovered that science here is more stressful and competitive than back home. Scientists are often under a ...
Viral Vectors 101: Chemogenetics

Type

Blog Post

Published

July 11, 2023, 1:15 p.m.

... This blog post was originally written by Maya Peters Kostman in May 2020 and updated by Jason Nasse ...

Showing: 141 - 160 of 218 results

Results per page:

Creating accessible biology activities in schools with BioBits

New and Upcoming Viral Vectors - May 2020

Developing a cold tolerant E. coli using specialized chaperones

GCE4All: Making Genetic Code Expansion Accessible

Viral Vectors 101: Viruses as Biological Tools

Viral Vectors 101: Gamma-Retroviral Packaging Systems

Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

CRISPRainbow and Genome Visualization

Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

To Each HIS Own

Summer SciComm: Preprints

Addgene's Tips for Plasmid Quality Control

5 Steps to Writing Grant Proposals for Grad Students

Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

A Look Back at One Year of Plasmid Sharing for COVID-19 Research

Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

Hot Plasmids and Viral Preps - January 2021

Meet an Addgenie: Insights into Grad School, Career Paths, and More

Viral Vectors 101: Chemogenetics

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