...In July 2016, we launched our Viral Service and began delivering ready-to-use lentivirus and adeno-associated... virus (AAV) to scientists around the world. We began with only a few inventory items offered domestically...PMCID: PMC3785459. 2. Krashes MJ, Koda S, Ye C, Rogan SC, Adams AC, Cusher DS, Maratos-Flier E, Roth BL...

...stakeholders, the product managers collate and organize that information and form it into a roadmap--a... features, programs, or tools to build. After organizing the information, a software product manager works...everything running smoothly, you’ll want to be really organized. Often, product managers will have three or more...

...proteomic mapping in both cell culture and whole organisms (see our 2019 overview). Until recently, the approach...dedicated transgenic line. Using zebrafish as a model organism, the Parton Lab recently created a flexible system...Given that the liver is an immunologically active organ, this advance is likely to afford the ability to...

... at Addgene, ComSciCon, and many more great organizations. At ComSciCon, our primary mission is to strengthen...respective fields. To help attendees launch new organizations that will extend opportunities to practice science... create them.  Seek out collaborations with organizations that practice science communication or start...

...engineer a specific function in these multicellular organisms. However, the rewards are great if you take the...are needed to increase the body of knowledge. Organizations like Open Plant can then build on this research...plant circadian clock. Petunias have been a model organism for plant research for decades, and the biochemical...

...defined as the inability of an organism to synthesize a particular organic compound required for its growth...DNA transformation system.Unlike most other microorganisms, yeast have both a stable haploid and diploid...

...like to start a group mentoring program at your organization, contact me! I can provide a step-by-step, how-to... people from different labs, departments or organizations as it brings a diversity of perspective. Sometimes...transition. Schedule your first meeting and get organized. Discuss your general areas of interest and what...

...More…” menu. You can also follow companies and organizations to see their news and job postings. 4) Use ...connections can connect you to people at that organization. This type of connection can help you gather...that is not relevant to business or volunteer organizational work. LinkedIn is not the platform for politics...

...celebrate! As we enter this new growth phase of the organization and the expansion of our impact, I’d like to...service and contribution! Leadership On an organizational level, we keep passing our audits with flying...even via Twitter. We love working with local organizations like Just-a-Start, the Northeastern co-op program...

...conjugating a CRBN-binding ligand (thalidomide) to an FKBP12F36V binding ligand (ortho-AP1867) via various...molecule is dTAG-13 due its immediate action, potent ligand performance, great depth of degradation, absolute...

...functional correction of the gene in clonally expanded organoids. This work, and many others, pushed the field ... post was originally written in 2014 by Kendall Morgan and updated in 2023 by Susanna Stroik.  References...of CFTR by CRISPR/Cas9 in Intestinal Stem Cell Organoids of Cystic Fibrosis Patients." Cell Stem Cell. ...

...concentrations and transients in living cells and organisms. Unlike chemical indicators, these DNA constructs...constructs can be delivered to intact tissues and organs noninvasively and target a defined population of cells...Genetically encoded fluorescent indicators for organellar calcium imaging. Biophysical journal. 2016 Sep...

...of NHEJ, HDR, and MMEJ repair varies from organism to organism. MMEJ doesn’t yield a “perfect repair” like...researchers can exploit for CRISPR gene editing. In organisms where HDR is downregulated, MMEJ represents another...

...tech transfer team will communicate with your organization’s technology transfer office or other authorized.... rerio (zebrafish), D. melanogaster (fly), C. elegans (nematode), S. cerevisiae (budding yeast), S. pombe.... The name of the Principal Investigator and Organization where your plasmids were first created. From...

...to deliver a standard AdV vector to the targeted organs or tissues with Cas9 and a gRNA designed to target...67 Ding Q, Strong A, Patel KM, Ng S-L, Gosis BS, Regan SN, Cowan CA, Rader DJ, Musunuru K (2014) Permanent...  Kendall Morgan...

...in 720°, i.e. they are on a membrane or in an organelle, then their effective concentration will be much...the membranes into stacked structures including organized smooth endoplasmic reticulum (OSER) whorls. 2)...distorted into dense stacked membranes termed Organized Smooth Endoplasmic Reticulum or OSER if a dimerizing...

...a co-founder of the BioBricks Foundation, an organization working to advance biological engineering openly... technical challenge. We have a strategy that organizes how we start the work, and it begins with the ... Kendall Morgan...

...slices, which are easier to expand than, say, a C. elegans (Damstra, et al. 2022; Yu, et al. 2020). With any...//doi.org/10.7554/elife.73775  Dani A, Huang B, Bergan J, et al (2010) Superresolution Imaging of Chemical...Wassie AT, et al (2020) Expansion microscopy of C. elegans. Elife 9:e46249. https://doi.org/10.7554/elife.46249...

...Chromatography columns containing appropriate immobilized ligands are incubated with cell culture supernatant containing...antibodies. The antibodies bind the immobilized ligands and can be eluted once the other materials have...incubated with the cell suspension. Antibodies bind ligands on the beads and a magnet is used to separate the...

...generated using random genomic fragments from the organism of interest, or a defined cDNA library (e.g. the...number of two-hybrid systems available in model organisms, including bacteria, alternative fungi (C. albicans...Enzymol. 1983;100:293-308. PubMed PMID: 6312261. 2. Keegan, L., Gill, G., and Ptashne, M. Separation of DNA...