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Showing: 141 - 160 of 499 results

  1. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    Published
    July 27, 2021, 1:15 p.m.
    ...internal control and a SL-sensitive fluorophore (circularly permuted green fluorescent protein or cpGFP) ... New from the viral service By Jason Nasse We regularly add new viral aliquots from our plasmid collection...

  2. Cellosaurus: A Cell Line Information Database

    Type
    Blog Post
    Published
    Feb. 21, 2023, 2:15 p.m.
    ...new resources with the scientific community – particularly ones that help address ongoing problems, like...growing cell line information database describing nearly 145,000 cell lines. Cellosaurus Cellosaurus seeks...

  3. Addgene at the ASM Microbe 2016 Conference

    Type
    Blog Post
    Published
    July 7, 2016, 2:30 p.m.
    ...Labeling Plasmids from the Barbier lab). Bright and early on Saturday morning, John Doench’s opening presentation... to the scientific community. However, from the nearly 4000 posters presented at the conference, it looks...

  5. 10 Ways to Share Your Science!

    Type
    Blog Post
    Published
    May 13, 2014, 8:34 p.m.
    ...some science on the one you already have. Read my earlier post about Scientists on Twitter here. 4)   If ...commitment, start your own blog. Plan to write regularly and, when you are ready, tell your friends or ...

  6. Donations from Addgene to Yield Answers for Rare Disease Researchers

    Type
    Blog Post
    Published
    Sept. 15, 2015, 2:30 p.m.
    ...by mutations in the OCRL1 gene that leads to the early death of young patients as their kidneys fail. The...interested in XP out of a desire to understand the early events in the transformation of healthy cells into...

  7. New and Upcoming Viral Vectors - December 2019

    Type
    Blog Post
    Published
    Dec. 19, 2019, 2:40 p.m.
    ...We have also packaged hChR2(H134R) plasmids from Karl Deisseroth’s lab, which includes pAAV-hSyn Con/Fon...WPRE-SV40 124650 AAV9 pAAV-CamKIIa-C1V1(t/t)-mScarlet-KV2.1 55645 AAV5 pAAV-hSyn Con/Fon hChR2(H134R...

  8. What Do I Do Now? Academic v. Non-Academic Career Decisions

    Type
    Blog Post
    Published
    March 9, 2017, 3:40 p.m.
    ...better, no grant writing Job changes can happen regularly (every 4-6 years is common), but this can also...diverse options and how to take advantage of them early is one way to smooth your path to a fulfilling career...

  10. Safe Port in a Storm...How Addgene is Weathering the Pandemic

    Type
    Blog Post
    Published
    Sept. 17, 2020, 1:15 p.m.
    ...expected to be in this here and now. Our closure was earlier than most companies. Since the start of this crisis... to make most of our payroll for the spring and early summer months. We are also extremely grateful to...

  11. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    Published
    Jan. 25, 2018, 2:09 p.m.
    ...plasmid in NEB T7 SHuffle bacteria (C3029J). We are clearly NEB “fanboys” as the bacteria significantly enhance...biochemist in the Vanderbilt Eye Institute and is particularly interested in cell signaling.     Additional...

  12. Identifying Sequence Elements with SnapGene's Feature Database

    Type
    Blog Post
    Published
    Dec. 21, 2017, 2:06 p.m.
    ...commercial plasmids were inconsistent and sometimes clearly wrong. In an effort to be rigorous, we dug into...Biology at the University of Chicago. He was an early depositor at Addgene, and conceived SnapGene because...

  13. What the HEK?

    Type
    Blog Post
    Published
    April 18, 2023, 1:15 p.m.
    ...two areas of research where HEK cells may be particularly useful due to their intrinsic properties. ...by mycoplasma and bacteria, but HEKs are also particularly vulnerable to viral infection. Yes, your cells...

  14. Single-cell tracking of lineage and identity with CellTag

    Type
    Blog Post
    Published
    May 9, 2019, 1:15 p.m.
    ...time point, making it difficult to investigate how early expression patterns influence reprogramming outcomes...However, distinct transcriptional states were seen as early as day 6. All together these results suggest that...

  15. Addgene Welcomes John Doench to the Advisory Board

    Type
    Blog Post
    Published
    Aug. 13, 2015, 2:30 p.m.
    ...specificity in the miRNA regulatory process. This early research is still relevant to his work at the Broad...screens to learn more about cancer biology in Ed Harlow’s group at Harvard Medical School and then applied...

  16. Celebrate National Book Month with Addgene

    Type
    Blog Post
    Published
    Oct. 3, 2019, 12:47 p.m.
    ...insects, mammals, and plants, among others. I particularly enjoyed the emphasis that microbes are neither...last 8 years. Since then, we’ve read books across nearly every genre you can imagine: gothic, mystery, sci-fi...

  18. The MTA at Addgene

    Type
    Blog Post
    Published
    July 23, 2024, 2:17 p.m.
    ...MTAs by the Provider and Recipient (in Addgene parlance, the depositor and requestor) organizations. Over...depends on things being organized, tracked, and clearly communicated. And that’s where MTAs, and similar...

  19. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    Published
    Oct. 19, 2023, 1:15 p.m.
    ...because they demonstrate high promoter activity (Earley et al. 2020), but non-AAV2 ITRs can be used to ...Addgene's AVV guide AAV Viral Preps References  Earley, L. F., Conatser, L. M., Lue, V. M., Dobbins, A...
Showing: 141 - 160 of 499 results