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Showing: 141 - 160 of 343 results

  1. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    Published
    Dec. 15, 2017, 2:07 p.m.
    ...difficult for individual labs to make efficiently. For select viral vectors in the repository, you can instead...in the brain, mapping neuronal networks, and selectively activating signaling pathways. Many scientists...

  2. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    Published
    Dec. 12, 2019, 2:22 p.m.
    ...terms in a search bar, by ticking some boxes, by selecting a suggestion — how should these be supported and...viral prep filter for promoter “Syn”, that, when selected, will match variations present in the data such...

  3. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    Published
    June 27, 2019, 12:25 p.m.
    ... contain the RP4 transfer origin as well as a selectable marker and a desired genetic payload (ex: GFP...sorted using FACS and isolated with antibiotic selection. The team tested this system in vitro and in vivo...

  4. What's New in CRISPR - November 2021

    Type
    Blog Post
    Published
    Nov. 18, 2021, 2:15 p.m.
    ...Chemical Biology Find the AsCas12f1 plasmids   Selective endogenous encapsidation for cellular delivery...sequences in the capsid, in an approach called Selective Endogenous eNcapsidation for cellular Delivery...

  5. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    Published
    July 14, 2021, 1:15 p.m.
    ...production of monoclonal antibodies starts with selecting just one of these B cell clones for further antibody...vials of the same lot of polyclonal antibody, or select a monoclonal or recombinant antibody instead. Otherwise...

  6. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    Published
    May 30, 2023, 1:15 p.m.
    ... or Cre dependent gene switches, and serotype selection. Promoters and serotype control, respectively,...AAVs down, you’re ready to take the next step – selecting your AAV and AAV components (serotype and promoter...

  7. To Each HIS Own

    Type
    Blog Post
    Published
    March 2, 2023, 2:15 p.m.
    ...substrates such as Ni2+ and are therefore more selective. When using systems with higher levels of histidine-containing...production systems, consider using these more selective substrates along with stringent wash conditions...

  8. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    Published
    Oct. 20, 2022, 1:15 p.m.
    ... gRNA. These gRNAs can be cleaved and rendered useless after less than a minute of exposure to the appropriate...CRISPR resources Request ready-to-use viral preps of select CRISPR lentiviral plasmids Additional resources...

  11. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

    Type
    Blog Post
    Published
    April 6, 2021, 12:30 p.m.
    ...confirm promoters, tags, fusion proteins, and selectable markers by detecting common features using Snapgene...sequence all plasmids in the pool but instead selectively sequence the variable portion of the plasmid ...

  12. Rabies and Neuronal Tracing

    Type
    Blog Post
    Published
    May 29, 2018, 1:51 p.m.
    ... al., 2013). Ultimately, this allows RABV to selectively infect a genetically defined cell population....RABV transsynaptic spread can be controlled by selectively supplying the G protein in trans only to starter...

  13. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    Published
    Aug. 10, 2023, 1 p.m.
    ... as well as newly developed calcium sensors. Selecting a calcium sensor Genetically encoded calcium indicators...is a great fit out there waiting for you. When selecting a sensor to use for your studies, you can start...

  14. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    Published
    July 11, 2023, 1:15 p.m.
    ...Using PSAMs PSAMs PSAMs, or pharmacologically selective actuator modules, were developed with the goal...specifically and potently activated by pharmacologically selective effector molecules (PSEMs). Higher potency means...

  15. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    Published
    Nov. 14, 2024, 2:15 p.m.
    ...fully understood, so it is important to carefully select the target locations of knock-in studies to avoid.../Cas13, which targets RNA instead of DNA and selectively degrades RNA molecules. This system is often ...

  16. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    Published
    March 8, 2017, 3:15 p.m.
    ...anything dangerous or toxic to humans in bacteria, select “yes” in the drop-down and contact Addgene. We ...prohibit Addgene from distributing the plasmid, select “yes” from the drop-down and add an explanation...

  17. Plasmids 101: Modular Cloning

    Type
    Blog Post
    Published
    March 12, 2024, 1:15 p.m.
    ...includes promoters, UTRs, terminators, etc. Once selected, individual units can be combined into… Transcription...systems as well as additional components such as selectable markers, promoters, enhancers, etc. MoClo is ...

  18. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    Published
    Nov. 7, 2017, 1:59 p.m.
    ...shuffling, and peptide displays. These capsids will be selected both for their ability to evade the immune system...specificity through control of gene expression and the selection of specific promoters may also help AAV vectors...

  19. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    Published
    Aug. 9, 2022, 1:15 p.m.
    ... Fig. 2: Schematic of the goldilocks antibody selection process The exposure time of the primary antibody...resources on the Addgene blog Antibodies 101: ValidationSelecting the Right AntibodyPlasmid-based Recombinant...

  20. Hot Plasmids: Winter 2025

    Type
    Blog Post
    Published
    March 11, 2025, 1:15 p.m.
    ... Figure 3: ASAP5 for voltage imaging. A) ASAP5 selected for its improved kinetics and responsivity in ...context-dependent responses and molecular patterns of selectivity. With 10 plasmids available as a kit, plus 21...
Showing: 141 - 160 of 343 results