...To: Plan Your Experiment Overview Select Genetic Manipulation Select Expression System Design gRNA Publications...mammalian cell lines Select Your Target Sequence and Design Your gRNA Once you have selected your CRISPR components...where the edit should occur and select a target sequence nearby. Select gRNAs based on predicted on-target...that are available for your particular experiment. Select Your Desired Genetic Manipulation Different genetic...manipulations require different CRISPR components. Selecting a specific genetic manipulation can be a good ... exist, including the multi-plasmid SAM system. Select Expression System To use CRISPR, you will need ...promoter for Cas enzyme and gRNA Presence of a selectable marker (drug or fluorophore) Delivery method ...

...efficiency than NHEJ. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Browse CRISPR Plasmids ...directed evolution. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Nick CRISPR/Cas nickase...repair (HDR). ID Plasmid Gene/Insert Promoter Selectable Marker Publication Prime Edit Cas9 H840A nickase... RT template. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Activate Catalytically ...specific locus. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Interfere Catalytically...specific locus. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Epigenetics To make targeted...heritable in dividing cells. ID Plasmid Gene/Insert Selectable Marker PI Publication RNA Knockdown Type VI CRISPR...

...transfected. Alternatively, you can use a selection marker to select transfected cells (for example, plasmid...your HR template has a selection marker such as puromycin, you can (also) select for the positive colonies...sequence. Because the 'NGG' of the PAM is used to select your genomic target, you need to make sure the ...protospacer followed by PAM. Click here for a target selection tool that should be helpful in minimizing or testing...clone in your target guides, test run them and then select the best guide to co-transfect in HR donor. Alternatively.../abstract/S0092-8674(13)01015-5 As for target selection, we usually pick 3-6 guides around the region ... might still be okay if you are introducing a selection marker gene. The homology arms should be no more...

...Insert Promoter Selectable Marker PI Publication Empty gRNA Expression Vectors Select a gRNA expression... efficiency than NHEJ. ID Plasmid Gene/Insert Selectable Marker PI Publication Base Edit Catalytically...strand) mutations. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Nick CRISPR/Cas nickase...repair (HDR). ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Prime Edit Cas9 H840A nickase... RT template. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Activate Catalytically ...specific locus. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Interfere Catalytically...expression plasmid based on factors such as selectable marker or cloning method. When using CRISPR, you will...

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...Insert Promoter Selectable Marker PI Publication Empty gRNA Expression Vectors Select a gRNA expression...efficiency than NHEJ. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Base Edit Catalytically...strand) mutations. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Nick CRISPR/Cas nickase.... ID Plasmid Description Gene/Insert Promoter Selectable Marker PI Publication Activate Catalytically ...specific locus. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Interfere Catalytically...specific locus. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Purify A catalytically ...expression plasmid based on factors such as selectable marker or cloning method. When using CRISPR, you will...

...nuclei, AF488, AF555, and AF647 Antibody Selection Selecting the right antibody for your experiment is...section to help you select antibodies for your approach. Primary antibodies To select an antibody, first...Methods Cell Sorting Methods Multiplex Assays Selection Validation Affinity reagents are molecular tools...mid-forties. When designing a multiplex assay, selection of the fluorophores used is important. It is best...appropriate lasers and filters for each fluorophore you select. Suggested panels for small multiplex experiments... you may either purchase several to compare, or select one based on price, shipping availability, ease... called secondaries, are usually much easier to select. There are three main considerations. What is the...

...Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert Promoter Selectable Marker PI...Promoter Selectable Marker PI Publication Drosophila ID Plasmid Gene/Insert Promoter Selectable Marker ...Publication Empty Prime Editing gRNA Vectors A selection of empty gRNA vectors suitable for prime editing...pegRNA, epegRNA, nicking sgRNA), cloning enzyme, selectable marker, and whether the plasmid contains Cas9...Guide RNA Type Cloning Enzyme Co-expressed Cas9 Selection PI 132777 pU6-pegRNA-GG-acceptor Mammalian hU6...

... you can find or select for only the cells that received the plasmid. Selectable Marker...curated by species, epitope tags/fusion protein, selectable marker, viral expression, and more. Plasmid...Species-specific expression Epitope tag or fusion protein Selectable markers Viral expression and packaging Reporters... our list of common tags . Back to Top Selectable Markers Regardless of your delivery method, it's...retroviral gene expression, multiple selectable markers pMKO.1 puro - Mammalian ... retroviral gene expression with GFP selectable marker LZRS-Rfa or pMXs-gw - Mammalian...

...expression of shRNA; neomycin selection; plasmid 21915 has puromycin selection TetR On Wiederschain 41393...miR-shRNA; selection cassette in format: rtTA3+IRES+Neo; see article for additional selection options third-generation...for inducible expression for Gateway cloning; selection cassette in format: PGK-rtTA-2A-puro; see article...luciferase in cloning site; see article for additional selection and FP option rtTA On Kowarz 16623 pBI-GFP Expression..., and Puromycin was replaced with Blasticidin selection. Please NOTE, this vector contains an insert (...

...expression of shRNA; puromycin selection. See plasmid 21916 for Neo selection. Wiederschain 11578 pSico 3rd...with EGFP marker. See plasmid 14749 for Thy1.1 selection. Benoist and Mathis 21915 Tet-pLKO-puro 3rd inducible...shRNAs or miRNAs, and use a wide variety of drug selection markers. See article for more variations. Campeau...-puro 3rd CMV driven expression of cDNA. Puro selection. Shih 25737 pSLIK-Hygro 3rd Tet-based inducible...Tet-pLKO-neo 3rd inducible expression of shRNA; neomycin selection. See plasmid 21915 for Puro resistance. Wiederschain...

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... and SpCas9, but does not contain markers for selection 1 . Other constructs may be utilized, such as ...ID 48139), which include GFP and puromycin as selectable markers, respectively, or constructs in which...Screening CRISPR/Cas9 Clones for Deletions and Clone Selection For suspension cells, transfer all clones to a...conditions optimized on the bulk cells (see step 6 ). Select the clones identified with the desired deletion...onto LB agar plates with the relevant antibiotic. Select multiple colonies, mini-prep each one, and subject...initial screen ensures that the correct clone was selected and reproducibility of results. To evaluate the...

...removing Stop sequences; Puro selection. See plasmid 35688 for Hygro selection. Mammalian Jacks 11925 pBS302...We also offer ready-to-use viral preparations of select Cre expression plasmids. Addgene's viral service...pBS-OMP-CreFNF OMP targeting vector with Cre FNF (neo-selectable marker flanked by FRT sites) Mammalian Mombaerts...IRES-CreFNF M71 targeting vector with IRES CreFNF (neo-selectable marker flanked by FRT sites) Mammalian Mombaerts...evaluating infection/transfection efficiency, selecting desired clones, and making transgenic mice. ID...Hughes 16422 betaRBPIG Double fluorescent, double selectable cre/loxP reporter Mammalian Hescheler 12463 p231...

...(eg GFP, Flag, YFP, etc) and selection markers. Two independent selection markers are available. An improved... repair) donor plasmid with homology arms and selection marker. The first deposited plasmids in this CRISPR-Cas... the Cas9-programming sgRNA and HR donors for selectable genome modification. This system can be used ...

...This tool can identify positively or negatively selected sgRNAs, genes, or pathways. CrispyCrunch CrispyCrunch...Tool (EuPaGDT) gRNA design tool with extensive selection of eukaryotic pathogen genomes (200+) that can...PDF 355.3 KB Goldstein Nematode: Injection and selection for Cas9-triggered homologous recombination Cas9...Cas9-sgRNA target construct ; pMA122 (negative selection marker); pGH8 (neuronal co-injection marker); ...

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