...these fragments can be cloned into E. coli and subsequent proteins screened for cell-binding abilities ...proteins directly onto the nitrocellulose for subsequent probing for cell-binding activity.  First, cut...room temperature. After this, it is helpful to subject your membrane to several freeze/thaw cycles to ...

...antigen to myeloma cells. The hybridomas are then subcloned by limiting dilution, which produces an immortalized...based on molecular weight by SDS-PAGE, and are subsequently transferred to a membrane. A primary antibody...of antibodies is used to both identify the cell subsets of interest from among the mixture and to measure...

...Fluorescence refers to the emission of light by a substance (fluorophore) that has absorbed light or electromagnetic...variant tag that covalently reacts with a small substrate ligand motif attached to a fluorophore. When paired...on a protein, enabling the labeling of protein substrates/peptides and antibodies. Example applications...

...is it? What parts of it move when you feed it substrate or add an essential cofactor? How many binding...especially since the potential rewards could be substantial: Plasmid tRNA Synthetase Expression Host ...Ermolenko, Dmitri N., et al. "Observation of intersubunit movement of the ribosome in solution using FRET...

...contain various promoters and reporters, and subsequently inserted into a Cas9-containing destination ...inserted. The BsaI site remains intact, allowing subsequent “bricks” to be added one by one. This approach...time needed for a single round of editing, and a subsequent curing protocol that requires only 2-3 hours ...

...phosphatase treatment, substituting cytidine with 5-methylcytidine, and/or substituting uridine with pseudouridine...selection is removed. Yu et al found that ⅔ of subclones generated from EBNA1 iPSCs still had episome DNA...

...four! (Note: For cases where the same name was submitted by more than one person, we selected the finalist...finalist based on the first time of submission.)     We love the way the names Aavery, Addy, and Cappy...

...over, we will host other contests in the future. Subscribe to the blog to stay in the know!     No pranks...see a reference to this scavenger hunt and the subsequent clue. (Begin with clue 1 below!) You will find...

... last week perusing the Deck The Lab contest submissions on Twitter and Instagram and we’re excited to... 17, 2019   Lab material tree We often get submissions of decorations created from lab materials. This...

...fantastic team of high school students who recently submitted to the journal with funding from Addgene. Listen...personal networking!) and the manuscript review/submission process at JEI. Chapter 3 - JEI target audience...

...Fluorescent Protein Plasmids 101: Visualizing Subcellular Structures and Organelles Resources at Addgene.org...Plasmids Find Plasmids for FRET Find Plasmids for Subcellular Localization ...

...entry will reveal additional data including the subject’s age at the time of injection and how long the ...contributed data so far and we encourage you to submit data from your experiments. You will be acknowledged...

... critical systems that cannot be removed or substantially reduced in an attempt to save energy. The transition....  Additional resources on the Addgene blog Subscribe to the Addgene blog Read our sustainability in...

...you time and improve your experiment results! Subscribe to the Addgene YouTube page! AAV purification ...lab tips, and more video content from Addgene, subscribe to Addgene’s YouTube Channel. Additional resources...

... Addgene office as we peruse the #DeckTheLab submissions. We asked all of the Addgenies to pick their ...Nika Heijmans There were many science-themed submissions: string lights hung up in the shape of RNA, light-up...

...hypothesis was that trained muscle would produce a substance with beneficial effects on the brain,” Ruas said...produces an enzyme that purges the body of harmful substances. So in this context the muscle's function is ...

... A KTR is comprised of a fluorescently-tagged substrate for a kinase of interest which is further fused... used to measure kinase activity in specific subcellular compartments. Second, because KTRs shuttle between...

...Experiments by Jennifer Tsang Chromoproteins are a subset of the fluorescent protein family and they generate...molecular biology tools out there! Here, we highlight a subset of these tools for plasmid mapping, DNA/protein...

...targeting the mouse and human kinomes. While any subset of the genome is limited by definition, the kinome...plasmids contain the same 3-4 gRNAs found in the first subset of the pooled library. The individual plasmids ...

...a few species of bacteria and sometimes, only a subset of strains of the same bacterial species are affected...developments at Phage Directory and in the phage field, subscribe to their newsletter, Capsid and Tail. Additional...