...Similarly, if you want to knock out a gene, a frameshift mutation introduced anywhere within the early...with NG PAM compatibility, as the name implies (Nishimasu et. al, 2018). xCas9 was generated by the Liu ...10.1126/science.aba8853 (Plasmids available here)   Nishimasu, H., et al.,Engineered CRISPR-Cas9 nuclease with...

...– $30,000 for research and another $30,000 in mentorship and business training. The money is a drop in...thinking about making the switch from academia to entrepreneurship, share your thoughts with us in the comments...guest bloggers! Nikolai Braun grew up outside of Washington D.C. and has always enjoyed exploring the outdoors...

...environment sooner rather than later can be helpful. Publishing: As a postdoc at AstraZeneca, you are expected...vary depending on which company you choose. If publishing is important to you, make sure that your project...an industry setting. Scientific community and mentorship: As an industry postdoc, you might find yourself...

... situation where one wants to understand the relationship between the age of a cell, protein, or cellular...: 1585-1588. PubMed PMID: 11090358. 2. Tsuboi, Takashi, et al. "Age-dependent preferential dense-core ... 118-126. PubMed PMID: 19136976. 5. Miyatsuka, Takeshi, Zhongmei Li, and Michael S. German. "Chronology...

...construct targeting vectors. Addgene depositor Takashi Yamamoto’s lab has harnessed MMEJ to create a new...NHEJ joins the ends of a DSB in an error-prone fashion, with insertions and deletions common. In contrast...possibilities MMEJ can enable. References 1. Sakuma, Tatsushi, et al. (2016).  “MMEJ-assisted gene knock-in ...

...and Challenges CUREs inspire students to take ownership of their work and become more actively involved...Biology Department at Keene State College in New Hampshire. He mentors undergraduates conducting NIH- and...):ar55. Ma, Y., Mukherjee, S., Uzzi, B. 2020. Mentorship and protégé success in STEM fields. Proceedings...

...equally effective are shipped to customers on ice, preventing any temperature shifts. Of course, we are ...Wang et. al., 2018). Aggregation can arise from a shift in temperature, pH, or salt concentration (Amin ...

...employees. The teambuilding culture helps us build relationships and thereby work better together.I realize I...encourage everyone to make an effort to build relationships with their colleagues. It can be easy to get...sometimes fun needs to be had in an orderly and timely fashion. 9. Ask for help My grandma used the old adage...

...as we obtain more genomic data about plasmids (Shintani et al., 2015). Now, bioinformatics tools help ...10.1016/j.sbi.2011.11.001 Pubmed PMID: 22153351 Shintani M, Sanchez ZK, Kimbara K (2015) Genomics of microbial....2015.00242 Pubmed PMID: 25873913 Uraji M, Suzuki K, Yoshida K (2002) A novel plasmid curing method using incompatibility...

...structure with regard to the Mentor-Singleton relationship. Some of the variability was organic, as some...coffee breaks. While this was not the more intense mentorship experience that happened at ASM Microbe, we connected...Science’s (SACNAS) annual conference all foster mentorship in different but successful ways. Scientific ...

...could have a shipping sprint and all the stories will touch different parts of the shipping code. The following...that the checkout takes the user directly to the shipping step, and no longer takes the user to a terms ...

...thanks to our guest blogger, Kusumika (Kushi) Mukherjee. Kusumika (Kushi) Mukherjee is currently a postdoctoral...This post was contributed by Kusumika (Kushi) Mukherjee, a Postdoc at Massachussetts General Hospital...

...Sadowski 1985). The FLP system works in a similar fashion to the Cre system. The FLP recombinase recognizes...inversion, and translocation of DNA in a similar fashion as between loxP sites (For details see Cre-lox ...the human brain.     Acknowledgement:Through a partnership with genOway, we are able to distribute materials...

...come under this ruling, regardless of visa or fellowship status, in addition to certain staff scientists...Department of Labor (for example here and here) pushing for exemptions for postdocs, and lowering of the...set up other institutions (like University of Washington) but as discussed elsewhere, there seems no basis...

...activities depending on the expertise of the senior leadership and members of their consulting staff. Who would... tasks, and then executing on them in a timely fashion while allowing for creative solutions to emerge...performance, and occasionally being faced with “shifting goal posts” as a part of a case, consulting can...

... the service has grown steadily, helped by a partnership with the University of Pennsylvania’s Vector ...2023. And while viral vectors are not as easy to ship as plasmids, they still have a global reach. From...this drove Addgene to develop new systems, new partnerships, and new platforms, from our early engagement...

...light of the policy, hold onto data longer before publishing. But there are strong incentives to publish such...data initiatives are also taking root. Nature Publishing Group recently launched a publication called ...veracity of collections and interpretation of data, publishing complete data, rather than just a single projection...

...differences, they had a hard time maintaining their friendships. I put “neutral” in quotations here because I...because that can indicate confusion around... Ownership: Misunderstandings about who is responsible for...for ScientistsTransferable Skills Guide: LeadershipTransferable Skills Guide: Teamwork  ...

...external video recorder and a mic clipped to her shirt. She rolled several long takes, and we cut it up... videos like this would prove to be difficult. Shipping the gear, and walking everyone through the recording...recorder using a lav mic, clipped to the talent’s shirt. But knowing I would have multiple interview subjects...

...multiple sources of error (red) influence these relationships and must be addressed with separate controls...accurately quantified In western blotting, the relationship between protein abundance and band intensity... BioRender.com. To see why this S-shaped relationship poses problems for proteins of very different...