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Showing: 141 - 160 of 490 results
  1. The Future of Research Symposium Boston 2015

    Type
    Blog Post
    Published
    Oct. 20, 2015, 2:30 p.m.
    ...will discuss where better data is needed to help shape and inform the overall structure of the biomedical...area include publishing the book ‘How Economics Shapes Science’ and contributing to the NIH’s Biomedical...PMCID: PMC4000813. 2. McDowell, Gary S., et al. "Shaping the Future of Research: a perspective from junior...
  2. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    Published
    Sept. 22, 2015, 2:30 p.m.
    ... barcodes within the resistant populations were shared by at least one other replicate; prior to treatment... “I spend a lot of time packing, shipping, and sharing protocols one by one,” and she is excited to deposit...Addgene because it is a “really great way of broadly sharing these kinds of reagents.” Here at Addgene, we’re...
  3. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

    Type
    Blog Post
    Published
    April 22, 2015, 2:06 p.m.
    ...are different types of cellulose fibers, pattern, shape. All of that is controlled by regulatory networks...same guide RNA twice. The second problem was the shape of the response function or how the output changes...function of input. Usually that function is in the shape of an S; there is a narrow threshold like a switch...
  4. Rabies and Neuronal Tracing

    Type
    Blog Post
    Published
    May 29, 2018, 1:51 p.m.
    ...retrograde transmission (Etessami et al., 2000, Wickersham et al., 2007). Thus, RABV transsynaptic spread...due to lack of G protein on the viral envelope (Wickersham et al., 2007).   Summary and RABV applications...Jun 7;74(5):858-73. Pubmed PMID 22681690.  8, Wickersham IR, Finke S, Conzelmann KK, Callaway EM. Retrograde...
  5. Lab Automation at Addgene

    Type
    Blog Post
    Published
    March 22, 2018, 1:28 p.m.
    ... Since Addgene is very proud to help scientists share plasmids across the globe, we’re working on ways...great lab automation tips and tricks, be sure to share them in the comments section.   Additional Resources... Shannon Rinaldi...
  6. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    Published
    Nov. 7, 2017, 1:59 p.m.
    ...Many thanks to our guest blogger, Harshana S De Silva Feelixge. Harshana S De Silva Feelixge is a researcher...This post was contributed by guest blogger Harshana S De Silva Feelixge. Gene therapy technologies ...
  7. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    Published
    July 18, 2019, 12:55 p.m.
    ...Wormbase. And scientists have been continuously sharing their worm strains through the Caenorhabditis Genetics...C. elegans researchers embrace open science and share resources and tools. That message was fully exemplified...some examples of worm resources and tools openly shared by the worm community. For additional resources...
  8. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

    Type
    Blog Post
    Published
    July 23, 2020, 1:20 p.m.
    ...difficult-to-engineer Sulfolobus islandicus rod-shaped virus 2 (Mayo-Muñoz et al., 2018). Only viral particles...CRISPR-Based Genome Editing of Sulfolobus islandicus Rod-Shaped Virus 2. Viruses 10:695 . https://doi.org/10.3390...Zhang Y, Garcia B, Hidalgo-Reyes Y, Lee J, Edraki A, Shah M, Sontheimer EJ, Maxwell KL, Davidson AR (2016)...
  9. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    Published
    Oct. 8, 2024, 1:15 p.m.
    ...metabolic pathways. Fertility (F) plasmids: microbial sharing F plasmids contain the genes for bacteria to form...new cloning techniques based on these vectors (Shareck et al., 2004). Plasmid categorization is complex... https://doi.org/10.1016/j.plasmid.2023.102684 Shareck, J., Choi, Y., Lee, B., & Miguez, C. B. (2004)....
  10. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    Published
    June 22, 2023, 1:15 p.m.
    ...experiments involve needles or scalpels (sharps). Cuts and scrapes from sharp equipment are one of the most common... yourself via needlestick or cut. Following all sharps procedures for your organization will significantly...
  11. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

    Type
    Blog Post
    Published
    Sept. 13, 2016, 2:30 p.m.
    ...engaged in, these issues which will continue to shape the sector throughout their careers (McDowell et...available avenue. Interestingly, both of these changes share a common thread that has the potential to profoundly...PMCID: PMC4866822. 3. McDowell, Gary S., et al. "Shaping the Future of Research: a perspective from junior...
  12. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    Published
    Dec. 1, 2015, 3:30 p.m.
    ...speak about Addgene’s experience helping scientists share plasmids, especially those of the CRISPR variety...outskirts of Guangzhouon west of beautiful Mao Fengshan Forest Park. As a national science and technology...distinction of “higher” and “lower” life forms as we shared millions of years of common ancestry with worms...
  13. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    Published
    Oct. 23, 2018, 11:49 a.m.
    ...? Please share them in the comments below! Special thanks for Dr. Dan Stone and Harshana de Silva ...definitely made my life easier!  In this post, I’ll share some AAV production, purification, and titration...
  14. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    Published
    Sept. 24, 2024, 1:15 p.m.
    ... social media) and freely sharing code, raw data, and more. The team shares results early and often, including...impact,” he says, noting that his team regularly shares troubleshooting tips and works with other labs ...
  15. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    Published
    July 16, 2015, 2:30 p.m.
    ...concentration of fluorophores) cast shadows along the illumination path. The shadows appear as stripes in the acquired...Klymkowsky. (1989) Development, 105.61-74. Pubmed. J. Sharpe, U. Ahlgren, P. Perry, Hill B, Ross A, J. Hecksher-Sorensen...
  16. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    Published
    Feb. 6, 2020, 3 p.m.
    ...teleconferencing software. Any file sharing service should also serve to share the materials quickly and easily...easily ahead of and after the journal club. Sharing the materials serve two purposes: to allow those who...
  17. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    Published
    July 9, 2019, 12:52 p.m.
    ...for parents and other members of the community to share their expertise with the kids through some fun activities...Middle school: supplementing the science curriculum Sharing my expertise with our district middle school was... of the talk by running it by the teachers and sharing some slides on Twitter, where I was able to get...
  18. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

    Type
    Blog Post
    Published
    Feb. 18, 2020, 2:15 p.m.
    ...when bacteria of different evolutionary lineages share genes that can improve chances of survival for an...experiences of the mentors were very valuable when shared with the next generation of scientists. The connection...Tiffany Lowe-Power, Eric Griffis, Janet Iwasa, Shawnna Buttery, Kat Milligan-Myhre, and Pragati Chengappa...
  19. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    Published
    Nov. 19, 2020, 2:15 p.m.
    ...Resource Center for Rett Syndrome research. With a shared passion for facilitating research and discovery...Novartis and rebranded to Novartis Gene Therapies, and Taysha Gene Therapies, founded on Gray’s work, are each...their MECP2-related plasmid tools with Addgene and share additional resources. Plasmid tools to facilitate...
  20. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    Published
    Sept. 16, 2020, 1 p.m.
    ...Gootenberg JS, Kriz AJ, Zetsche B, Shalem O, Wu X, Makarova KS, Koonin EV, Sharp PA, Zhang F (2015) In vivo genome...KLM, Chuck J, Tan D, Knott GJ, Harrington LB, Al-Shayeb B, Wagner A, Brötzmann J, Staahl BT, Taylor KL,...
Showing: 141 - 160 of 490 results