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  2. Your Top Requested Plasmid in 2016!

    Type
    Blog Post
    Published
    Jan. 11, 2017, 8:47 p.m.
    ...plasmid from 2016 is an expression vector for CRISPR nuclease, SpCas9 from the Zhang Lab. It's name, though ...restriction sites into which you can clone your 20 nucleotide guide sequence of choice (for generation of a...

  5. Our Most Popular Blog Posts from 2019

    Type
    Blog Post
    Published
    Dec. 31, 2019, 2:26 p.m.
    ...Soup by Aliyah Weinstein RNA aptamers, which are nucleic acid sequences that fluoresce upon binding a small...Transfection by Alyssa Cecchetelli The introduction of nucleic acids into bacteria or eukaryotic cells is a common...

  6. What's New in CRISPR - September 2019

    Type
    Blog Post
    Published
    Sept. 10, 2019, 12:56 p.m.
    ...addition of 4-hydroxytamoxifen, it can move to the nucleus for activity. They also developed one of these ...gene activation and editing. Read the paper in Nucleic Acids Research Find the drug inducible Cas9 plasmids...

  7. What's New in CRISPR - March 2020

    Type
    Blog Post
    Published
    March 10, 2020, 1:15 p.m.
    ...repressor Mxi1, and an optogenetic tool that controls nuclear localization (LINuS). Blue light renders the NLS...NLS accessible and the complex enters the nucleus where dCas9-Mxi1 can repress gene expression. In the...

  8. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    Published
    June 30, 2015, 2 p.m.
    ...and binds the inverted repeats (green); and an endonuclease reaction occurs which cuts the DNA.3.) The released...transposon can now bind a strand of DNA with a TA dinucleotide (there are many such sites in the human genome...

  10. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    Published
    Oct. 27, 2016, 2:30 p.m.
    ...green cloud) functions both as a restriction endonuclease and as a ligase by cleaving and rejoining supercoiled... 2. Novel approach to molecular cloning and polynucleotide synthesis using vaccinia DNA topoisomerase....

  11. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    Published
    Aug. 11, 2020, 1:15 p.m.
    ...tertiary structure when transcribed which aids in nuclear export of mRNA and helps increase the expression...The polyadenylation or polyA signal aids in the nuclear export of RNA and RNA translation, and promotes...

  14. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

    Type
    Blog Post
    Published
    April 7, 2015, 4:21 p.m.
    ...catalytically dead Cas9 protein (dCas9) that lacks endonuclease activity coupled with a customizable single ...RNA (scRNA) will bind to a PAM-adjacent target nucleotide sequence then subsequently recruit a transcriptional...

  15. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    Published
    April 18, 2019, 12:53 p.m.
    ...chromosomes and how these arrangements may affect nuclear processes. The latest of these visualization tools...the gRNA in living cells. Broccoli aptamers are nucleic acid sequences that bind to the small molecule,...

  16. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    Published
    March 14, 2019, 1:54 p.m.
    ...contain other cellular molecules such as proteins or nucleic acids. This conjunction of vectors and exosome ...contain cellular macromolecules (e.g. proteins, nucleic acids) which possibly improve transduction properties...

  18. DAP arrays for multiplex gene editing

    Type
    Blog Post
    Published
    July 7, 2022, 1:15 p.m.
    ...and has previously been used for multiplex Cas9-nuclease editing. Typically, a single array encoding the...editing, such as base editing, prime editing, and nuclease editing, in virtually any cell type or organisms...

  19. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    Published
    Oct. 19, 2023, 1:15 p.m.
    ....com. ITR structure  ITRs are symmetrical 145-nucleotide sequences that flank the ends of the single stranded...Figure 2). Additionally, the ITR contains a 4-nucleotide Rep binding element (RBE) that serves as a binding...

  20. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    Published
    Sept. 6, 2017, 1:01 p.m.
    ...incorporation of a 3’-5’ exonuclease in the reaction mixture. The exonuclease removes mismatched 3’ overhangs...
Showing: 141 - 160 of 321 results