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  1. Nuclear Receptor Signaling Atlas (NURSA) Plasmid Guide: Nuclear Receptors

    Type
    Collection
    ... alpha PPARD Plasmids PPARD, FAAR, NR1C2, NUC1, NUCI, NUCII, PPARB peroxisome proliferator-activated receptor...Browse the nuclear receptor plasmid collection assembled in collaboration with the Nuclear Receptor Signaling... Plasmid Collections Nuclear Receptor Plasmid Collection Nuclear Receptor Plasmid Collection You...Wikipedia, accessed November 6, 2013. Author: Boghog2 Nuclear receptors are a class of transcription factors ...modulated by ligands. Two of the important regions of nuclear receptors are: 1) a ligand binding domain, which...activation or repression of target gene expression. Nuclear receptors play a key role in development and homeostasis...from around the globe, Addgene has assembled a Nuclear Receptor plasmid collection for the community. ...

  2. Zinc Finger Consortium: Nuclease Expression Vectors

    Type
    Collection
    ...Zinc Finger Consortium Nuclease Expression Vectors Zinc Finger Consortium: Nuclease Expression Vectors This...page lists plasmid vectors designed to express nucleases in different cell types. Zinc finger arrays can...page lists plasmid vectors designed to express nucleases in different cell types. Zinc finger arrays created...cloning sites. Vectors encoding wild-type FokI nuclease domains: pST1374 (for expression in mammalian ...in plants) Vectors encoding heterodimeric FokI nuclease domains: pMLM290 (for expression of a “+” heterodimeric... “-” heterodimeric FokI domain ( Sollu et al., Nucleic Acids Res ) in mammalian cells or zebrafish). pCLR2068...by Moore et al. PLoS One 2012 and Cade et al., Nucleic Acids Res 2012 ) pCLR2070 (for expression of a ...

  3. Joung Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) Plasmids

    Type
    Collection
    ...target genomic DNA sequence and a nuclease (e.g.—the Cas9 endonuclease) that cleaves the target DNA. The...CRISPR-Cas9 nucleases with altered PAM specificities Dimeric CRISPR RNA-guided FokI Nucleases (RFNs) CRISPR-Cas...Joung Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) Plasmids Genome...Plasmids Joung Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) Plasmids You may also like... CRISPR Guide CRISPR...gRNA Design Tools CRISPR Blog Posts RNA-Guided Nucleases ( RGNs ), based on naturally occurring Type II...II CRISPR-Cas systems, are programmable endonucleases that can be used to perform targeted genome editing...target sites and to facilitate design of short oligonucleotides needed to construct gRNA expression vectors...

  4. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...pCaggs-NLS-PAmCherry1-GSS-EGFP Nucleus NLS mGold Francois St-Pierre 14893 pEBFP2-Nuc Nucleus NLS EBFP2 Robert Campbell...pmKalama1-Nuc Nucleus NLS mKalama1 Robert Campbell 73205 pcDNA3.1-NLS-myc-dL5-2xG4S-mCer3 Nucleus NLS (from...Cytosol, nucleoplasm None dL5 FAP; mCerulean3 Marcel Bruchez 36206 pmTurquoise2-NES Non-nucleus Nuclear Export...Non-nucleus Nuclear Export Sequence mScarlet-I Dorus Gadella 85061 pmScarlet-H_NES_C1 Non-nucleus Nuclear...3xnls-mNeonGreen Nucleus 3xNLS mNeonGreen Dorus Gadella 98817 3xnls-mTurquoise2 Nucleus 3xNLS mTurquoise2...pEGFP-C1-Fibrillarin Nucleolus Fibrillarin EGFP Sui Huang 36207 pmTurquoise2-H2A Nucleus H2A mTurquoise2 Dorus...pmScarlet-i_H2A_C1 Nucleus H2A mScarlet-I Dorus Gadella 85052 pmScarlet-H_H2A_C1 Nucleus H2A mScarlet-H Dorus...

  5. Control AAV Preps

    Type
    Collection
    ...PHP.eB, PHP.S Boyden 112677 pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP EF1a NLS-mCherry or nls-EGFP Cre...

  6. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...Fluorescent Protein Guide NLS Nuclear Localization pENTR4-myc-nuc - pEntry vector that...that adds a C-terminal nuclear localization signal FNpcDNA3 - N-terminal ... FLAG and SV40 nuclear localization signal Myr Membrane Localization pWZL-Neo-Myr-Flag-DEST - ...

  7. Retrograde AAV viral preps

    Type
    Collection
    ...EYFP Control Gradinaru 112677 pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP EF1a Color-flipping switch. Expresses...GCaMP6f-P2A-nls-dTomato Syn GCaMP6f and physically separate nuclear dTomato Calcium sensor Ting 51083 AAV-EF1a-DIO-...AAV-EF1a-DIO-GCaMP6f-P2A-nls-dTomato EF1a GCaMP6f and physically separate nuclear dTomato, Cre-dependent Calcium sensor Ting 83899...GCaMP6s-P2A-nls-dTomato Syn GCaMP6s and physically separate nuclear dTomato Calcium sensor Ting 100853 pAAV.Syn.Flex.NES-jRGECO1a.WPRE.SV40...

  8. CRISPR Guide

    Type
    Collection
    ...like zinc finger nucleases (ZFNs) or transcription-activator-like effector nucleases (TALENs) required...popular Cas endonuclease, with many modified versions engineered to increase specificity and nuclease activity...increase nuclease fidelity SpCas9-NG - NG; increase in vitro activity SpG - NGN; increase nuclease activity...break; the wild type function of most Cas nucleases dCas9 Nuclease dead Cas9, an enzymatically inactive form...required scientists to design and generate a new nuclease pair for every genomic target. Due to its comparative...RNA ( gRNA or sgRNA ) and a CRISPR-associated endonuclease (Cas enzyme). The gRNA is a short synthetic ...necessary for Cas-binding and a user-defined ∼20-nucleotide spacer that defines the genomic target to be ...

  9. Immunology Research Plasmids and Resources

    Type
    Collection
    ...proliferator-activated receptor delta FAAR, MGC3931, NR1C2, NUC1, NUCI, NUCII, PPAR-beta, PPARB PPARG peroxisome proliferator-activated...HLALS NFYA nuclear transcription factor Y, alpha CBF-A, CBF-B, FLJ11236, HAP2, NF-YA NFYB nuclear transcription...FLJ37489 VAV1 vav 1 guanine nucleotide exchange factor VAV VAV2 vav 2 guanine nucleotide exchange factor - VAV3...NROB1 NR0B2 nuclear receptor subfamily 0, group B, member 2 FLJ17090, SHP, SHP1 NR1D1 nuclear receptor subfamily...hRev NR1D2 nuclear receptor subfamily 1, group D, member 2 BD73, EAR-1R, RVR NR1H2 nuclear receptor subfamily... UNR NR1H3 nuclear receptor subfamily 1, group H, member 3 LXR-a, LXRA, RLD-1 NR1H4 nuclear receptor subfamily...MGC97144, MGC97209 NR2C1 nuclear receptor subfamily 2, group C, member 1 TR2 NR2C2 nuclear receptor subfamily...

  10. Allen Institute for Cell Science Plasmid Collection

    Type
    Collection
    ...mTagRFP-T Nucleophosmin Nucleolus (granular component) 133963 UBTF-HaloTag AICSDP-80 HaloTag Nucleolar transcription...Golgi 109122 NPM1-mEGFP AICSDP-50 mEGFP Nucleophosmin Nucleolus (granular component) 109120 GJA1-mEGFP ...Cohesins 114407 NUP153-mEGFP AICSDP-50 mEGFP Nucleoporin Nup153 Nuclear pores 114410 FUS-mEGFP AICSDP-64 mEGFP...transcription factor UBF Nucleolus (fibrillar center) 133964 SON-mEGFP AICSDP-82 mEGFP SON Nuclear speckles 159744...Microtubules 87422 LMNB1-mEGFP AICSDP-10 mEGFP Lamin B1 Nuclear envelope 87423 TOMM20-mEGFP AICSDP-8 mEGFP Tom20...reticulum 87427 FBL-mEGFP AICSDP-13 mEGFP Fibrillarin Nucleolus (dense fibrillar component) 87428 MYH10-mEGFP ...114403 LMNB1-mTagRFP-T AICSDP-29 mTagRFPT Lamin B1 Nuclear envelope 114404 AAVS1-mEGFP (PGK) AICSDP-36 mEGFP...

  11. Open Enzyme Collection

    Type
    Collection
    ...glycosylase, aka T4 Endonuclease V) 165540 pOpen-T7EI T7 Endonuclease I 165551 pOpen-Endonuclease IV E. coli E... E. coli Endonuclease IV 165561 pOpen-Endonuclease VIII E. coli E. coli Endonuclease VIII 165571 pOpen-Exonuclease...pOpen-Exonuclease III E. coli E. coli Exonuclease III 165559 pOpen-T5gene12/13 T5 Exonuclease 165560 pOpen-T4...Collection DNA Ligases DNA Polymerases Restriction Endonucleases Reverse Transcriptases RNA Polymerases Other...below: DNA Ligases DNA Polymerases Restriction Endonucleases Reverse Transcriptases RNA Polymerases Other...phi29 DNA Polymerase Return to top Restriction Endonucleases Addgene ID Plasmid Gene/insert 165504 pOpen-...methyltransferase 165520 pOpen-T4 PNK T4 PNK polynucleotide kinase 165521 pOpen-lambda red operon lambda...

  12. TALEN Guide

    Type
    Collection
    ...a zinc finger (ZF) nuclease. Zinc fingers recognize triplets and the FokI nuclease operates as a dimer...sites. (B) A TAL effector nuclease (TALEN). Similar in principle to the ZF nuclease, the components of the...the array recognize individual nucleotides. For over ten years, zinc finger (ZF) arrays have been the go-to...number of zinc fingers that recognize various nucleotide triplets. With some trial and error, ZF arrays...years of research has shown that when fused to a nuclease or activation domain, a ZF array is an effective...without their limitations, however. Not every nucleotide triplet has a corresponding zinc finger, and ...Bogdanove at Iowa State University published the nucleotide recognition code of the TAL effectors, which ...

  13. Brain Initiative Collection

    Type
    Collection
    ...10aa-H2B-WPRE3-BGHpA AAV vector for nuclear SYFP2 labeling in neurons. Useful for nuclear isolation and scRNA-seq ...pAAV-hDlx-GiDREADD-dTomato-Fishell-5 Gi-DREADD (P2A) nuclear dTomato expression in forebrain GABA-ergic interneurons...pAAV-hDlx-GqDREADD-dTomato-Fishell-4 Gq-DREADD (P2A) nuclear dTomato expression in forebrain GABA-ergic interneurons...

  14. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...genome engineering technologies like zinc finger nucleases (ZFNs) and TALENs. CRISPR: An Adaptive Immune ...Through sequence homology, these crRNAs guide a Cas nuclease to the specified exogenous genetic material, which...the individual crRNAs. (3) The tracrRNA and Cas9 nuclease form a complex with each individual, unique crRNA... alternative PAM sites . Cpf1 , another CRISPR nuclease, recognizes a 5’-TTN PAM, improving targeting ...Cascio VM, Joung JK. 2014. Improving CRISPR-Cas nuclease specificity using truncated guide RNAs. Nat Biotechnol...Shinagawa H, Makino K, Amemura M, Nakata A. 1987. Nucleotide sequence of the iap gene, responsible for alkaline... E. 2012. A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity. Science . 337...

  15. Zhang Lab CRISPR Page

    Type
    Collection
    ... CRISPR-mediated nucleic acid cleavage. We have harnessed the type II CRISPR nuclease system to facilitate...nterspaced S hort P alindromic R epeats) is a microbial nuclease system involved in defense against invading phages...by co-expressing the S. pyogenes Cas9 (SpCas9) nuclease along with the guide RNA. For most applications... have found that adding an additional guanine nucleotide to the 5’ of 20-bp guide can increase targeting... genes. It consists of three components: A nucleolytically inactive Cas9-VP64 fusion A sgRNA incorporating... have found that adding an additional guanine nucleotide to the 5’ of 21-bp guide can increase targeting...GFP-KASH fusion facilitates FACS sorting of cells and nuclei. Return to top AAV - Cas9 mouse CRISPR-Cas9 is ...

  16. Ras Pathway

    Type
    Collection
    ...NF1 Neurofibromin 1 NFE2L2 Nuclear factor, erythroid 2 like 2 NFKB1 Nuclear factor of kappa light polypeptide...Ral guanine nucleotide dissociation stimulator RAPGEF RAPGEF1 RAPGEF2 Rap guanine nucleotide exchange factor...GTPase and is a member of the G protein (guanine nucleotide binding protein) family. GTPases serve as molecular...activating protein 35 ARHGEF2 Rho/Rac guanine nucleotide exchange factor 2 CCND CCND1 CCND2 CCND3 Cyclin... RASGRF1 RASGRF2 Ras protein specific guanine nucleotide releasing factor RASGRP RASGRP1 RASGRP2 RASGRP3...scaffold protein SOS SOS1 SOS2 SOS Ras/Rho guanine nucleotide exchange factor SPRED SPRED1 SPRED2 SPRED3 Sprouty...TSC1 TSC2 Tuberous sclerosis VAV1 Vav guanine nucleotide exchange factor 1 YAP1 Yes associated protein...

  17. COVID-19 Resources

    Type
    Collection
    ...targeting the SARS-CoV-2 nucleocapsid protein (Brian Geiss). Anti-SARS-CoV-2 Nucleocapsid Protein [mBG86] - ...detect nucleic acids at the atomolar level. For more information read Addgene's Blog: Finding nucleic acids...monoclonal antibodies that target SARS-CoV-2 nucleocapsid protein. SARS-CoV-2 Plasmids : Plasmids that...COVID-19 research. Antibodies Anti-SARS-CoV-2 Nucleocapsid Protein [mBG17] - Clone mBG17, recombinant mouse...monoclonal antibody targeting the SARS-CoV-2 nucleocapsid protein (Brian Geiss). Plasmids SARS-CoV-2 Plasmids...degradation to develop the method termed DNA Endonuclease Targeted CRISPR Trans Reporter (DETECTR). Mammoth...

  18. Plan Your Experiment

    Type
    Collection
    ...off-target editing is a major concern, Cas9-gRNA ribonucleoprotein (RNP) complexes are advantageous due to the... to generate transgenic embryos Cas9-gRNA ribonucleoprotein (RNP) complexes Purified Cas9 protein and ...increase or decrease depending upon the specific nucleotides within the selected target sequence. For example...example, gRNA targeting sequences containing a G nucleotide at position 20 (1 bp upstream of the PAM) may...be more efficacious than gRNAs containing a C nucleotide at the same position in spite of being a perfect...products are denatured-renatured, treated with a nuclease that cleaves DNA heteroduplexes, and run on an...restriction digest (for HDR repaired DSBs): For small nucleotide edits that introduce a novel restriction site...

  19. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...40822 EGFP-alphasynuclein-WT SNCA GFP CMV Parkinson's David Rubinsztein 40823 EGFP-alphasynuclein-A53T SNCA... pHM6-alphasynuclein-WT SNCA His, HA CMV Parkinson's David Rubinsztein 40825 pHM6-alphasynuclein-A53T ... Alpha-synuclein SNCA T7 Parkinson's Michael J Fox Foundation MJFF 51486 pET21a-alpha-synuclein SNCA T7...Alpha-synuclein SNCA T7 Parkinson's Michael J Fox Foundation MJFF 89074 pD454-SR rat Alpha-synuclein SNCA...Alpha-synuclein SNCA T7 Parkinson's Michael J Fox Foundation MJFF 89076 pD434-SR rat Alpha-synuclein SNCA...89470 VN-alpha Synuclein SNCA Venus CMV Parkinson's Bradley Hyman 89471 alpha Synuclein-VC SNCA Venus ...89483 VN-beta Synuclein SNCB Venus CMV Lewy body dementia Tiago Outeiro 89484 beta Synuclein-VC SNCB Venus...

  20. FlyCRISPR

    Type
    Collection
    ...site sequence can be added by inserting an oligonucleotide linker into BsaI digested vector. Cloning targeting... of Drosophila with the CRISPR RNA-guided Cas9 nuclease. Gratz SJ, Cummings AM, Nguyen JN, Hamm DC, Donohue... 46294 pBS-Hsp70-Cas9 : A codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter.... 45945 pHsp70-Cas9 : The codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter...
Showing: 1 - 20 of 67 results