...interested public.” Subscribers to Scientific America would be considered the interested public and it would...Image courtesy of Ron Mader, via Flickr, under the Creative Commons license     ...podcasts A series of Tweets breaking down a new publication that could advance treatments for a rare disease...emails, most science communication work does not explicitly exclude people outside of the original recipients...

...orthologs similar to TtAgo and PfAgo, another thermophilic Argonaute. They identified NgAgo and showed ...template is supplied. Find Plasmids from the NgAgo Publication at Addgene Cas9 and NgAgo head to head NgAgo ...differences in cleavage efficiency. If this finding is replicated in larger studies, it will represent a key advantage...site specified by the gDNA. Depending on your application, this could be favorable or unfavorable. In a...PubMed PMID: 27136078. Find plasmids from this publication at Addgene. 2. Swarts, Daan C., et al.  “DNA-... PMCID: PMC4697943. Find plasmids from this publication at Addgene. 3. Swarts, Daan C., et al. “Argonaute...

...or DNA base pair mismatches (8-11). For some applications, there cannot be any end-joining activity, and...regions. Why use a high fidelity ligase? Numerous applications have been developed to take advantage of the...Taq and other thermostable DNA ligases. These applications detect specific nucleotide sequences with high...Ligase are not replacements for T4 DNA Ligase in applications such as routine cloning or DNA library preparation...al. "Ligase chain reaction (LCR)-overview and applications." PCR Methods Appl 3.4 (1994): S51-64. PubMed... detection system for direct identification of allelic composition of mutated DNA in a mixed population...to accelerate characterization of nucleic acid metabolic enzymes." Nucleic acids research 44.2 (2015): ...

...particles and the virus particles produced could not replicate. However, Mother Nature found a way to re-create...packaging plasmid forming a vector that could create replication competent virus. Even though this system was ...still a potential risk for the generation of replication competent lentiviruses (RCL) especially if you...factors in HIV positive cells could make the virus replication competent. The 3rd generation of lentiviral...transgenes (>8 kb) Potential for generation of replication-competent lentivirus (RCL) Efficient gene transfer...transfer Potential for insertional mutagenesis: Replication-incompetent lentiviruses with human tropism can... incubate your cells with virus at different multiplicity of infection (MOI=ratio of virus to cells). ...

...regions flanking the target site).  The best applications for dsDNA inserts include: large insertions ...targeted, it’s best to determine the direction of replication through your region of interest and design an...it’s easier to not determine the direction of replication and instead design oligos that target both strands...correct DNA mismatches that occur during DNA replication. There are two ways to avoid activating MMR: ...Uses in Recombineering Substrate Best Suited Application Recombination Frequency Special Considerations...from a plasmid with a heat sensitive origin of replication.  Once the recombineering system is no longer...defective prophage is to use mini-λ, a defective non-replicating, circular piece of phage DNA, that when introduced...

...need? Multiple first or corresponding author publications (published, not in preparation) A history of...The other items will follow. If you get more publications, you will be more competitive for fellowships... project. Furthermore, Group Leader position applications require 3 letters of reference- the graduate...spurious yet intriguing findings that fail to replicate. Plan experiments logically and strategically ...and exploration Researchers face a fundamental conflict. Science is about exploration, discovering new...informal recommendations, more positive grant application and manuscript reviews, etc. You'll also meet...teach classes. Therefore, seek out management and conflict resolution workshops. Get teaching experience ...

...tropism, replication kinetics, and cell damage profiling of SARS-CoV-2 and SARS-CoV with implications for ... Another example of reagents that have broad applications but have proven useful to the study of SARS-...vectors VSV-G and psPAX2, which were used in a publication by Xiuyuan Ou, et al. that characterized the ... requested these plasmids from Addgene. This publication then resulted in the development of a new plasmid...scientific community. Deposits of new reagents, policies from journals and granting agencies requiring ...

...objects that exist on the internet so that when you click on the link it will take you directly to the original...item. Given a specific Addgene ID # listed in a publication, you can quickly pull up the associated plasmid...plasmid page also helps researchers find other publications that have used the same plasmid through the ...section. Having IDs linked to these metadata and publications help to enable reproducibility of experiments...and N2T.net. To help authors comply with journal policies, Addgene now provides an RRID and a resolvable... in the material and methods section of your publication. You should carefully read and follow the journal...

...2020.  “CRISPR Gene Therapy: Applications, Limitations, and Implications for the Future.” Front. Oncol... for full details.    CRISPR advances in monoallelic diseases In 2013, a team led by Jinsong Li from...the technology in different labs for different applications.” Li’s team noted that CRISPR/Cas9 has been ...with size limitations. In addition, non-viral applications are also possible through the use of nanoparticles....  “CRISPR Genome Editing Technology and its Application in Genetic Diseases: A Review.” Current pharmaceutical...

...together a grant application? Are you familiar with the publishing or patent application processes? How ... pages in a Curricula Vitae to showcase your publications, academic performances, and research experiences...information. Think "skills," not just "research and publications” What are the skills you developed through your... science? Or perhaps you have presented in some public lectures? These transferable skills could be meaningful...make their job postings and salary expectations public, so start by looking at these university postings...

...transcription, or DNA repair, or replication, or any number of CRISPR applications and thought “OK, but how does...with Addgene, but you’ll find many great tools by clicking on the links below and visiting their associated...containing lists of plasmids from the indicated publications. You can request all of these plasmids directly..., Hoefler R, Schmalhorst PS, Jude JG, Zuber J, Gerlich DW Cell. 2017 Aug 24;170(5):956-972.e23 Screen ..., Hoefler R, Schmalhorst PS, Jude JG, Zuber J, Gerlich DW Cell. 2017 Aug 24;170(5):956-972.e23 Rapid and...

...protein for your application. Selecting the best fluorescent protein for a specific application can seem like...under conditions that best mimic the intended application. The three key properties for live cell imaging...experimental set up. The practical brightness takes all application specific parameters into account, including the... picture of what can be expected in a 'real' application than the theoretical brightness. Consequently...brightness in a system closely mimicking the future application. In figure 2 we provide an example of the comparison...

...your application a whole week before the deadline. The day of submission, review your application with... their dream.  To help students focus on the application process, and simplify access to academic resources...Scrutinize every opportunity prior to starting an application to assess your eligibility, interest, and career...February represent peak season for internship applications. Thus these noble individuals are inundated ... with a stellar letter of support.        5. Application submission  Set a calendar reminder for every...

... our knowledge. Disease Applications Q13: Does AAV have potential applications in diseases which may be... to use split-inteins to mediate trans-protein splicing. Note, however, that this will require co-delivery...reliable and reproducible. However, we do not use amplicons in ITR as we have found this to strongly bias ...1x1014 vg/ml.. As evident in our repository, this application is an outlier for PHP.eB (1x1011 - 1x1012 vg/...CRISPR tools is an exciting area for future AAV application to epigenetic editing. This area is discussed...

...tool that can be used for a variety of research applications. Within the immune system, antibodies are produced...antibodies to bind proteins is useful for research applications as well because they allow scientists to target...150 kDa, a nanobody is 12-15 kDa.   Research applications for antibodies You can use antibodies for experiments...Let’s dive in and take a look at some of these applications. Western blot Western blots are used to detect...immunocytochemistry). This could be cells or tissue slices mounted to a slide, or whole organs. Because the... by binding to cell surface receptors. These applications are typically used for investigating signaling...are out there, and how many different research applications they can be used for. Addgene’s Antibody Plasmid...

...ampicillin resistance cassette and an origin of replication, which are not schematized. Sizes of fragments...2024, used under the Creative Commons CC BY-NC-ND license.   Once you have the components you want, simply...validated and were chosen because of their broad applicability in different model systems. What’s the error...five months. When they tested those clones (in duplicate), 93% had the correct banding pattern in the restriction...correct. Because they created the assemblies in duplicate, they were able to confirm that at least one plasmid...design. Note that you can browse all components by clicking the blue text in the upper right-hand corner.  ...

...chemical replication of RNA in the absence of evolved enzymatic machinery (i.e. RNA replication with no...technology park, it enjoys preferential governmental policies for a high-tech development zone so many science...inheritance that might be less error prone than DNA replication. He too mentioned how “surprising” small RNAs...created a circRNA website so scientists can view public circRNA datasets and download the custom python...Steitz quipped that RNA in the ribosome is in a complicated conformation “not like DNA which has a boring...

...delivered with an enhanced pU6cm promoter, viral replicon amplification of cargo, and heat treatment.  ...2024 bioRxiv, under CC-BY-NC-ND 4.0 International license. With this approach, the team was able to drive...Arabidopsis, suggesting the improvements may be broadly applicable to dicot species. In addition, they generated...Adapted from Smith et al. 2024 under a CC-BY 4.0 license.  The team deposited an array of constructs...activation triggered by endogenous GPCR. Example applications include large-scale pharmacological profiling...adapted from Wu and Wang 2024 under a CC-BY 4.0 license. Find more neuroscience antibodies here! ...

...are, you’ll even have DNA left over for other applications, like PCR, cloning, transfection, or long-term...wash step or use a different strain. For most applications, Addgene uses and recommends DH5𝝰! This strain...your E. coli to ensure it is suitable for your application. We have previously reviewed many common lab ...partially specified by a plasmid’s origin of replication, but can also be affected by the plasmid size...the material used for inoculation. To further complicate matters, plasmid copy numbers are often ambiguous...volume limits, the copy number of some origins of replication (e.g. p15A, ColE1) can be increased by adding...

...about a month and a half, without the need for complicated cloning of homology arms. MMEJ: An Introduction... inserted into the PITCh vector via In-Fusion or SLIC cloning. Step 2: Design an insertion locus-specific... for making targeted modifications. A recent publication by Zhang et al. shows just that - using MMEJ ...lies in the diversity of nucleases and their applications. It will be interesting to see what new editing...PMID: 26678082. Find the plasmids from this publication at Addgene. 2. Dalvai, Mathieu, et al. (2015...633. PMID: 26456817. Find plasmids from this publication at Addgene. 3. Zhang, Chi, Xiuhua Meng, Xiaolei...