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...primary antibody controls in your experiments. If you do find high levels of background staining by the secondary...binding Direct immunohistochemistry (Figure 2A)  How do you avoid having your secondary antibody bind endogenous...common solutions to avoid background staining when doing species on species IHC. (A) One solution is to use...

...2016; Torres & Casadevall, 2008) but…does it really do anything? Fc functions The answer is, yes, it does...Fc effector functions and all that antibodies can do! For instance, some Fc receptors, once bound to an...structure and isotype determination, remember that it can do so much more than that!    References and resources...

...Enhancer traps do not need to be integrated within an intron or exon of a gene, and thus they do not disrupt...they are directly integrated into it. Enhancer traps do not need to be inside of a gene’s open reading frame...

...alone; a researcher doing some experiments in Brazil can have the same issues as one doing experiments in ...sometimes wish they could live differently than we do day to day. We discovered that it can be good for...

...with a PhD in biology. Depending on what you want to do, that’s not necessarily bad news, but it is an argument... science with the community. Some of them you can do today! 10 ways to share your science 1)   If you ...talk – really talk – about the research you are doing in the lab with your mother or father or your best...

...support your career development as your mentor. How do you make the most of this new relationship? Consider...skills in which you have expertise or knowledge. Where do these intersect? Work with resources: Choose books...take larger goals (e.g. Figure out what I want to do after I get my PhD) and break them down into smaller...

...questions! How much virus should I inject? How long do I need to wait? What serotype and promoter should...the answers, but we’re betting that your colleagues do. That’s why we’re launching the AAV Data Hub. The...

...the population that have an edit. I didn’t do this, but to do it you would need a pretty good gel image...Cas9-expression cell line. Especially if you’re doing a screen. I made a few monoclonal lines (Figure ...expansions (monoclonal, 1-5). Wild-type A549 cells do not express CAs9 and are included as a control (WT...couldn’t get a good image. You could probably also do Western blotting against GFP expression, but I didn...designing primers flanking my edits in genomic DNA and doing PCR on genomic DNA. The surveyor assay itself was...

...How do you know if you actually have DNA in your tube without seeing it? There are many ways to do this... time to set up in the lab, you might not have to do the calculation yourself as many fluorometers will...

...are the perfect person to be writing the review! Doing so is a great way to develop your ability to write...here, I will share my process with you. But before I do that, let me say that I was by no means an expert...overthink it. And to quote my adviser, "you just gotta do it." At the end of the day, someone has to write ...article and that someone is going to be you. So, just do it! I will also paraphrase what I have heard many...

...Satellite colonies are small colonies of bacteria which do not contain the plasmid and its associated resistance...effectively selecting plasmid-containing bacteria. So… why do some people still use amp? Carb is expensive (relative...Tn5 element, but thankfully, most competent cells do not express this factor. Zeocin is handy because ...above antibiotics would work for you, they likely do! Give our summary table a look for a quick recap ...

...explaining how your experience and skills would help you do the job as described. Let’s imagine you’re applying...example, if you don’t have experience with ddPCR, but do have experience with qPCR, you could talk about how...that are excited about their work and mission.  How do I know what to write about? If you’re struggling ...that skill. If the answer is yes, ask yourself “how do I know that I have that skill?” The answer to that...

...people develop the disease while others never do, but we do know that there are some genetic factors involved...much of a danger of getting the disease, even if you do happen to be exposed to asbestos. However, as the...cancer is diagnosed so late that these treatments do not work very well. The best option in such instances... mesothelioma many years later. While there is a dose-response relationship (the higher the exposure, ...

... why you might enjoy doing it. I’ll also talk about the skills you’ll need to do well as a healthcare ...healthcare consultant and what you could do to break into the field. What is healthcare consulting? Healthcare...pulling your weight with your scientific expertise. Do I have to stick with consulting in the long-term?...and services that help diabetic patients manage doses and timings of their drug treatments. The process...

...the situation in the future. Addgene: What advice do you give to post-docs and graduate students as they...publications in a short period of time and may need to do some fashionable projects. However, I hope that they... they also have long-term goals and continue to do research to realize their vision. Their progress might...they'll be completely different from what we are now doing in my lab then when they become independent. Looking...

...same transcript. Of course multicistronic vectors do not have to exclusively be used as a means of detection...levels between two or more genes is important. How do multicistronic vectors work? Scientists have "borrowed...commonly found in bicistronic vectors; however, they do have some disadvantages. These elements are quite...of the peptide to improve cleavage efficiency. How do I get started? If you are looking to co-express your...

...these ratios right, you need to do a few calculations. This calculator can do the math for you and it’s simple...

...collection (what science has been hot this year and how do you creatively turn it into an outfit?), and of course... box. Which is your favorite team costume so far? Do you have any suggestions for this year’s teams that...

...detection So you found mycoplasma contamination, what do you do next? The best way is to discard the infected ...contamination before they enter your cell culture and what to do if you encounter mycoplasma contamination. Causes... optical microscope can be very difficult, so how do you know it’s there? For this task, you can use PCR...before you even realize it. Here are some things to do: Wear a clean lab coat and mask while handling cell...

...individually or simultaneously. Do note for control purposes that there are dosage effects. For example, overexpression... of interest, the next question to ask is what to do with them. In some cases, there might be a mutation...will need to be overexpressed or knocked down. To do this, Drosophila geneticists use the Gal4/UAS system...