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Welcome to Addgene's blog! In our blog we'll help you share science by highlighting the latest plasmid technologies, bringing you the inside scoop from scientists, providing career development advice, and more.

Nov. 14, 2013, 4:10 p.m.

Looking for a way to test a lot of different parts in your cloning, or maybe interrogate some complex genetic circuits? MoClo just might be the approach for you!

March 12, 2024, 1:15 p.m.

Considering purifying a protein to study it? Learn all about the polyhistidine tag, or HIS, in this blog post!

March 2, 2023, 2:15 p.m.

GPCRs are receptors that respond to stimuli and elicit cellular responses. Learn about the parts of GPCRs, how they work, and tools to study them.

Feb. 11, 2021, 2:15 p.m.

Find tips for working with RNA and simple protocols for extracting RNA without using a kit

March 24, 2020, 1:15 p.m.

Learn how you can use RaPID to detect RNA-protein interactions and its applications in infectious and human genetic diseases.

Aug. 16, 2018, 12:40 p.m.

This post describes CRISPR-derived base editors that catalyze base transversions, such as purines like A or G to pyrimidines like T or C.

April 1, 2025, 1:15 p.m.

Optogenetics aren't just for neuroscientists! In this post, we review optogenetic tools for cytoskeleton and membrane control in cells.

Sept. 28, 2023, 1:15 p.m.

Dr. Michael Koeris is cofounder Biotech startup, Sample 6, and a member of the Addgene Board. Listen to his journey from grad student to entrepreneur.

Jan. 21, 2016, 4:52 p.m.

Learn about the csRNP epitope tagging method - a new and efficient way to tag endogenous genes using CRISPR.

June 28, 2018, 3:01 p.m.

Plasmid-encoded antibodies offers many advantages over other types of antibodies. Learn about how recombinant antibodies are made.

May 4, 2021, 1:15 p.m.

Discover the tools you need to perform plant genome engineering with CRISPR.

Oct. 11, 2016, 2:30 p.m.

Summary of degron tags along with their applications, advantages, and several commonly used tags.

March 14, 2024, 1:15 p.m.

An overview of split fluorescent proteins and a highlight of some of the most popular and newest ones available on Addgene.

May 3, 2022, 1:15 p.m.

an overview of qPCR and ddPCR copy number variant assays.

April 26, 2022, 1:15 p.m.

Recent highlights from Addgene's collection include tools to study SARS-CoV-2 antibody escape, antibodies for aminoacyl tRNA synthetases, and plasmids for generating nanocompartments for yeast bioproduction.

May 18, 2021, 1:15 p.m.

Learn some best practices to keep your mammalian cell lines free of contaminants and your experiments running smoothly.

July 12, 2018, 1:09 p.m.

Check out the latest Hot Plasmids, viral vectors, and antibodies in the Addgene repository!

March 11, 2025, 1:15 p.m.

A recent study shows ancestral bias in CRISPR guide libraries, leading to skewed cancer dependency results, particularly in cell lines with African ancestry.

Nov. 29, 2022, 2:15 p.m.

Find plasmid tools for COVID-19 research expressing viral open reading frames, host factors, and tools for detecting SARS-CoV-2 and generating pseudoviruses.

July 12, 2021, 1:15 p.m.