...for dynamic experiments in which FRET changes are large. Two other methods are commonly used to measure...

...have some disadvantages. These elements are quite large (500-600 bp) and may take up precious space in viral...

...transcription factors, therefore promoters play a large role in determining where and when your gene of ...

...using these viruses to mark neuronal connectivity a large majority of neuronal cells are labeled overtime ...based on its unique hue (plasmids at Addgene). A large-scale example of neuronal tracing with AAV vectors...

...factors - OCT3/4, SOX2, KLF4, C-MYC, LIN28 and SV40 large T antigen (SV40LT). The method had a reprogramming...research and clinical trials, as it allows access to a larger number of willing participants. While fibroblasts...

...by adding a nuclear localization sequence (SV40 Large T nuclear localization sequence) and expressing ...

...lead to asymmetrical plasmid loss of the usually large, low copy virulence plasmids. This strategy has ...partitioning machinery away from a larger, low copy plasmid and the larger, low copy plasmid is more likely...

...Measuring the Activity of Protein Variants on a Large Scale Using Deep Mutational Scanning.” Nature Protocols...

... linearized pEcgRNA backbone can be prepared in large amounts and frozen for future use or directly ligated...

... 3. Mashiko, Daisuke, et al. "Feasibility for a large scale mouse mutagenesis by injecting CRISPR/Cas ...

...thus dsDNA plasmids are generally preferred for large insertions such as fluorescent proteins or selection...

...difficult. The first-generation mARGs also had a large genetic footprint (two cassettes + a booster cassette...

...institution/funding source. Major equipment includes large centrifuges, sequencers and analyzers, and any ultra...

...changes in enzyme function can lead to surprisingly large fitness effects during adaptive evolution of antibiotic...

...bind to your biotinylated antibody. This creates a large meshwork of biotin-avidin complexes bound to your...

...something should be communicated is necessary in a large organization, because confirming that every person...

...blocks for engineered proteins. This flexibility is largely due to scFvs being smaller than antibodies. scFvs...

...Tomida, J., et al. "Detection of phosphorylation on large proteins by Western blotting using Phos-tag containing...

...cells compared to dead cells. As a result, the largely negative live cells can still be easily gated on...

...Both subunits have short intracellular ends and large ectodomains containing ligand-binding heads and ...

...frequency of 5%. Your experiment involves making a large sgRNA library to thousands of genes and targeting...

...modulate biological processes, but it did not show large changes in binding affinity with light stimulation...advantageous compared to other viral systems due to its large cargo capacity, minimal cytotoxicity, and broad ... CRISPR-Display uses the dCas9 enzyme to deploy large RNA cargos to specific DNA loci. In CRISPR-Display...

...on many different levels - it’s hard to process large amounts of information and, from a SciComm perspective...results. As biological researchers deal with larger and larger datasets and use computer based analysis techniques...

...models remain more difficult to make. For example, large insertions, like a fluorescent protein knock-in,...

...) NLS-Sa dCas9-NLS-LSD1. LSD1 is a particularly large protein, and this fusion allows for packaging into...genomic targets. These two vectors are part of the larger pMVP/pMAGIC cloning system, which contains Cas9...

... without causing issues associated with cloning large fragments. After fragmentation, repair sheared ends...

... to reduce false positive rates and control for large numbers of comparisons. The Bonferroni does this...arranging all calculated p values from smallest to largest and ranking them (1 being the smallest value). ...calculates the cut off for significance by finding the largest p value where its rank divided by the total number...

...therefore determines the timing of gene expression and largely defines the amount of recombinant protein that ...

...mechanism for sgRNA delivery and providing a means for large scale functional screens. For more information on... kinases Receptor tyrosine kinases (RTKs) are a large class of cell-surface receptors which play a critical...

...their environment. These types of interactions to a large extent involve receptors expressed on the cell surface...

...preferential tropism for different brain regions is largely due the ability of different serotypes to infect...is fine and a researcher should aim to inject a larger amount of vector using a serotype with strong CNS... of adeno-associated virus serotypes: impact of larger genomes on infectivity and postentry steps." Journal...

...The best applications for dsDNA inserts include: large insertions or deletions, including selectable DNA...

...require high read counts (fast multiplexing) Requires large cell population, senstive to time since cell fixing...; Pattanayak et al. 2013). This approach misses larger mismatches (e.g. six nucleotides) that may still...

...Getting a vector into a particular cell type depends largely on the viral capsid, but specificity doesn’t end...

...are sensitive to distortions in helix shape (22). Large purine:purine mismatches and most smaller pyrimidine...

... at the same time pass over in silence his very large one” (http://oll.libertyfund.org/titles/galilei-...that the community of science supporters is much larger and stronger that I expected. Jennifer Stancill...

...useful for intracellular staining where binding of large immune complexes may be hindered. Indirect staining...information about the relative size of the cell; larger cells produce more FSC. Light that scatters sideways...

...) with 30-60 basepair homology arms to knock in large epitopes, such as GFP (Paix et al., 2014). A further...

...can help reduce off-target activity. Deleting a large region of the genome by removing the sequence between...

...-resolution imaging, they still do not emit the large number of photons achievable with photoswitchable...