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  1. Plasmids 101: Protein Expression

    Type
    Blog Post
    Published
    June 7, 2018, 1:17 p.m.
    ...control protein expression for experimental, biotechnological, and medical applications. Researches can ...desired protein. Despite being a rather nascent technology, plant cells have been used to express a wide...

  2. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    Published
    Oct. 17, 2017, 12:55 p.m.
    ...physical models of proteins using 3D printing technology. The tagline for our company is “…where molecules...effective this model was in explaining CRISPR technology to 4th graders, check out Jennifer Doudna’s new...

  3. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    Published
    Sept. 7, 2017, 12:17 p.m.
    ...pipettor is set to 200 ul, wiped down with 70% ethanol, and a box of sterile truncated pipet tips (USA...where it immediately spreads out to form a firm monolayer. The cell clones - easily identified from the ...

  4. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    Published
    Feb. 11, 2025, 2:15 p.m.
    ...Yoshimi, K., & Mashimo, T. (2022). Genome editing technology and applications with the type I CRISPR system...for genome engineering in human cells. Nature Biotechnology, 37(12), 1471–1477. https://doi.org/10.1038/...

  5. Plasmids 101: 5 factors to help you choose the right cloning method

    Type
    Blog Post
    Published
    Aug. 21, 2018, 12:31 p.m.
    ...many labs still love using them! With so many technologies currently available, you now face the challenge...ligation-independent PCR cloning method." BMC biotechnology 11.1 (2011): 92. PubMed PMID: 21992524. PubMed...

  7. Addgene's Cancer Collection Pages Connect the Research Community

    Type
    Blog Post
    Published
    Sept. 6, 2016, 2:30 p.m.
    ... use with the Gateway Cloning Platform (Life Technologies, Carlsbad, CA) that represents 180 genes from...Scientists are making progress. New resources and technologies become available almost daily, yet obstacles...

  9. DNA Purification Without a Kit

    Type
    Blog Post
    Published
    Feb. 11, 2020, 2:15 p.m.
    ...RNase to remove RNA contamination and you can use a phenol chloroform extraction to remove remaining proteins...of four times before a wash with ddH2O and then ethanol. After regeneration, trace amounts of DNA (3 femtograms...

  10. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type
    Blog Post
    Published
    Oct. 4, 2018, 12:44 p.m.
    ...CRISPR-Cas9–mediated gene inactivation." Nature biotechnology 32.12 (2014): 1262. PubMed PMID: 25184501. PubMed... off-target effects of CRISPR-Cas9." Nature biotechnology 34.2 (2016): 184. PubMed PMID: 26780180. PubMed...

  11. MXS Chaining

    Type
    Blog Post
    Published
    Feb. 7, 2017, 3:30 p.m.
    ...proteins and the availability of powerful imaging technologies, the combination of multiple fluorescent protein...High Throughput Cloning Strategies Method Technology Pros Cons References Golden Gate Type II restriction...

  12. Getting to Know Expansion Microscopy

    Type
    Blog Post
    Published
    June 20, 2023, 1:15 p.m.
    ...anchoring strategy for expansion microscopy. Nat Biotechnol 1–12. https://doi.org/10.1038/s41587-022-01546...standard fluorescent proteins and antibodies. Nat Biotechnol 34:987–992. https://doi.org/10.1038/nbt.3625  ...

  13. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    Published
    July 31, 2018, 12:55 p.m.
    ... I quickly became fascinated by this emerging technology and its potential for the treatment of some genetic...research is being done to further improve the technology: engineering of new capsid variants, designing...

  14. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    Published
    May 15, 2014, 3:33 p.m.
    ... chromophore to form with an indole rather than phenol component (cyan derivatives) Y66H Blue-shifts... many other uses currently exist, and new GFP technology is constantly being developed! Fusion tagging...

  15. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    Published
    Sept. 16, 2020, 1 p.m.
    ...adeno-associated viral (AAV) vector toolbox. Biotechnology Journal 9:1402–1412 . https://doi.org/10.1002...in the mammalian brain using CRISPR-Cas9. Nat Biotechnol 33:102–106 . https://doi.org/10.1038/nbt.3055...

  16. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    Published
    Aug. 20, 2020, 2:30 p.m.
    ...specificities of Cpf1 endonucleases in human cells. Nat Biotechnol 34:863–868 . https://doi.org/10.1038/nbt.3609...CRISPR-Cas Cpf1 nucleases in human cells. Nat Biotechnol 34:869–874 . https://doi.org/10.1038/nbt.3620...

  17. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    Published
    Nov. 19, 2020, 2:15 p.m.
    ... and Feng Zhang, PhD, is pursuing CRISPR/Cas9 technology for precise correction of Rett syndrome mutations...three neuronal subtypes of the hippocampus. This technology has formed the basis of the Rett syndrome program...

  18. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    Published
    Nov. 12, 2020, 2:15 p.m.
    ... be used in some in vivo experiments and in biotechnology and therapeutics (Mauro and Chappell 2014).  ... heterologous protein expression. Trends in Biotechnology 22:346–353 . https://doi.org/10.1016/j.tibtech...

  19. Career Insights: Technical Support Specialist

    Type
    Blog Post
    Published
    Nov. 9, 2017, 2 p.m.
    ...necessary), there are opportunities to work for biotechnology companies and help other researchers use the...experience and scientific knowledge they require. Biotechnology companies vary to what extent they hire experts...

  20. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    Published
    March 12, 2019, 1:37 p.m.
    ... execute the proposal and the tools/resources/technology exist to solve the problem. What will you have...received his PhD in Molecular Microbiology and Immunology from Oregon Health Sciences University, did his...

  21. Lab to Office Culture Shock

    Type
    Blog Post
    Published
    Feb. 5, 2019, 1:04 p.m.
    ... my work. The meetings often are the work. Will Arnold, one of the newer members of our Scientist team...and for modeling in the picture above), and Will Arnold for their ideas and tips! Additional resources ...

  23. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    Published
    April 11, 2017, 2:30 p.m.
    ...Fluorescence resonance energy transfer (FRET) technology has been studied and used in assays for over ...PMC4441765. 4. Guo, Peixuan AND Haque, Farzin. RNA Nanotechnology and Therapeutics. Boca Raton: CRC Press, 2014...

  24. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    Published
    April 11, 2024, 1:15 p.m.
    ..., Methylophilus methyltrophus, Methylococcus methanolicus, Methylosinus trichosporium, Myxococcus xanthus...protein expression across gram negative hosts. Biotechnology and Bioengineering, 106(2), 326–332. https:/...

  25. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    Published
    Aug. 14, 2018, 12:42 p.m.
    ...also be used with the most cutting-edge imaging technologies such as array tomography, super-resolution STORM...W. Davidson. "Advances in fluorescent protein technology." Journal of cell science 120.24 (2007): 4247...

  26. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    Published
    Aug. 18, 2016, 2:30 p.m.
    ...discovery through the creation of innovative technologies. To date his group has develop several popular...genome editing and transcription modulation. Nat Biotechnol 33:139–142 . https://doi.org/10.1038/nbt.3149...

  27. The Challenges of Cell Culture

    Type
    Blog Post
    Published
    May 5, 2016, 2:30 p.m.
    ...tool used in the fields of oncology, virology, immunology, microbiology, and pharmacology (many of which...stance on the matter. Funding bodies such as the Biotechnology and Biological Sciences Research Council (BBSRC...

  29. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    Published
    May 5, 2020, 1:15 p.m.
    ...times, have led researchers to turn to other technologies like CRISPR. Figure 1: An overview of...CRISPR–Cas12-based detection of SARS-CoV-2. Nature Biotechnology. https://doi.org/10.1038/s41587-020-0513-4 Kellner...
Showing: 211 - 240 of 459 results