...requires ddPCR skills. Your resume lists ddPCR, so the hiring manager knows that you have ddPCR experience...with ddPCR, but do have experience with qPCR, you could talk about how your experience with qPCR would...that by describing how you developed and used a ddPCR assay to answer an experimental question. Now, the...manager knows that you can design experiments with ddPCR, interpret the results, and develop a successful...would make it easy to master ddPCR.  Finally, it’s a good idea to state why you’re applying to the specific...

...biosensors kit By Mike Lacy G protein-coupled receptors (GPCRs) are critical components of many physiological pathways...targets. To enable large-scale profiling of many GPCRs, Mikel Garcia-Marcos’ lab recently developed a collection... detection of activation triggered by endogenous GPCR. Example applications include large-scale pharmacological...The authors used this platform to assay dozens of GPCRs across many cell types in varying conditions, revealing...2024). Direct interrogation of context-dependent GPCR activity with a universal biosensor platform. Cell...

...titer results between ddPCR and qPCR? A: At CLOVER, we have traditionally used qPCR (protocol link). We ...this to strongly bias titer. We are also adopting ddPCR, which does not a standard curve and somewhat more...

...titration by qPCR using SYBR green technology Here’s some advice on how to successfully set up your qPCR to determine...generate a standard curve, visit the AAV Titration by qPCR written protocol page. Getting started with tissue...

...clinical and diagnostic labs, deep sequencing and qPCR methods are too costly and time intensive to process...platform on clinical stool samples by comparison to qPCR and demonstrated that it can also detect expression...mRNA expression cannot be identified with DNA-based qPCR methods, the ability to detect mRNA transcripts ...

...signaling pathways G protein-coupled receptors (GPCRs) detect molecules outside of the cell and translate...those signals into cellular responses. For example, GPCRs and their downstream G protein partners can regulate...have a long standing interest in the biology of GPCRs and G proteins, with an emphasis on oligomerization...

... diagnosing COVID-19, aside from the widely used qPCR and antigen tests.  Labs including Jennifer Doudna...Taq polymerase that can be used in a one-step RT-qPCR reaction to detect SARS-CoV-2 nucleic acids. Mammalian...

...designer drugs, are G protein-coupled receptors (GPCRs), that have been reprogrammed to respond only to... threshold and silence neurons. DREADD-activated GPCR signaling can also modify other properties such ...

... loop regions of any G-protein-coupled receptor (GPCR) recognizing a specific ligand (Ino et al. 2022)...produce more than 20 other MTRIA sensors based on GPCRs recognizing various neurotransmitters, neuropeptides...

...quality control processes as our in-stock service: ddPCR, purity testing, and sterility testing, plus viral...

...new optogenetic tool called PdCO.  This is an "optoGPCR" (coupled to Go, so it's an inhibitory opsin!)...

...assays we run with all of our in-stock viral preps: ddPCR, purity testing, sterility testing, and viral genomic...

...advantages Mapping the brainDissecting circuits Studying GPCR pathways   Acknowledgements We thank the Bryan...

...for downstream applications (Berns, 1972). Using qPCR and restriction enzyme based methods, we showed ...

...for the newest GRAB sensors from the Li Lab. These GPCR based sensors provide the spatiotemporal resolution...

... gRNAs can be expressed in a single vector, pCRISPRainbow-DONOR1, with dCas9 supplied by a separate vector...

... in gene expression readouts from assays such as qPCR or microarray. But, aside from facilitating the ...

...Rhodopsin GEVIs Rhodopsin is composed of an opsin (a GPCR) linked to a chromophore and functions as a photoreceptor...

...viral vector production facilities frequently use qPCR with a standard curve to titer their viruses; food...

... of the new viral preps from recent months: New GPCR activation-based norepinephrine (GRABNE) sensors...