...cell population. The region of interest is PCR amplified, PCR products are denatured-renatured, treated... The PCR product is then run on an agarose gel to determine whether the edit was successful. PCR amplification...run on an agarose gel to identify DNA fragments. PCR and restriction digest (for HDR repaired DSBs): For...novel restriction site. The region of interest is PCR amplified, digested with the appropriate restriction...run on an agarose gel to identify DNA fragments. PCR amplification and gel electrophoresis (for HDR or...deletions or insertions, the region of interest can be PCR amplified using primers that (A) flank the region...and exact sequence of targeted alleles. Involves PCR amplification of targeted region from DNA, subcloning...

...add restriction sites to any piece of DNA during PCR amplification, allowing for it to then be digested...a single adenosine (A) overhang on the 3' end of PCR products. The complementary thymine (T) comes from...an incredibly quick and easy way to clone a fresh PCR product into a plasmid. The major disadvantage of...are also available, allowing for rapid cloning of PCR products into donor plasmids without the need for...used, so if properly designed, any insert fragment (PCR product or synthesized oligo) with appropriate overhangs...add). Complementary overhangs are built into the PCR primers for the insert, based on the destination ...

... onto the insert of interest using PCR. This is accomplished by PCR amplifying the insert sequence using...restriction sites onto the insert of interest using PCR. The process of subcloning consists of digesting ...

...surviving cells are collected, their plasmids are PCR-amplified and sequenced using NGS to identify their...

...single-stranded DNA sequence used as an initiation point for PCR amplification or DNA sequencing of the plasmid. Primers...

... is necessary for Cas enzymes to bind target DNA PCR P olymerase C hain R eaction; Used to amplify a specific...

...coupled receptors, or GPCRs. The largest class of cell surface receptors, GPCRs are seven-pass transmembrane...receptors? Read the GPCRs: How Do They Work and How Do We Study Them? blog! Studies of GPCRs led to development...activates G-proteins to modulate downstream signaling. GPCRs have been shown to be involved in a wide variety...inflammation and neurotransmission. This, and the fact that GPCR ligands can have high potency, which is potentially...that couples to β-arrestin to activate noncanonical GPCR signaling independent of G proteins. This DREADD... manipulate neuronal activity indirectly through GPCR signaling, another class of chemogenetic receptors...