...the way. That’s what makes it so wonderful. The most challenging part of jumping into this career path...explainer of the who, what, where, when, why and most importantly, how, of a science policy career.  Who...engagement with your local or state governments. Most communities have opportunities to join commissions...do science policy? I would argue that this is the most important question that you can explore. The answer... important skills for the profession. One of the most important skill sets for a science policy career...

...research is rooted in the CNS, my focus will be mostly on the brain, but many of these tips apply to all... estate and using AAVs have in common? The three most important items when looking for a house and choosing...Well, an AAV user must determine which serotype is most efficient at transducing the cell type under study...influences on overall cell function. AAVs are the most appropriate vehicle for this type of research, as...zinc finger nucleases, and many others (12). The most attractive potential of AAVs is their ability to...

... pass on to you to make sure you are getting the most out of the data you get back from your sequencing...controls help you properly visualize your results.The most important of those is to always look closely at ...miscalled peak, don’t be shy. Feel free to edit it. Most chromatogram viewing programs (even the free ones...sequence.   I hope these tips will help you get the most out of your DNA sequencing results and to troubleshoot...

...become. One thing is for sure, as is obvious from our most requested plasmid, SpCas9 is still going strong ...editing experiments. So, without further ado, the most request plasmid in 2016 was... pSpCas9(BB)-2A-Puro... lines. Cas9, of course, is the key component of most CRISPR systems and generates double strange breaks...name are some additional features. The first and most imporant is that, upstream of the Cbh promoter, ...

...Centre for Regenerative Medicine (CRM), Edinburgh. Most commercial antibodies do not work in pull-down assays...diverse biochemical tasks in the cell. One of the most popular approaches to study protein interactions...methods (electroporation, lipofection etc) is the most crucial aspect of knock-in experiments. You’ll need...depends on many factors. Some of these include: Most importantly, the crRNA should cut near the insertion...with custom synthetic cr:tracrRNAs (csRNP) is the most efficient and convenient choice for epitope tagging...

... network of relationships early and often is the most effective tactic a scientist in training can adopt...professional (and personal) relationships. However, most job seekers will and should apply for jobs posted...get help if you are having trouble preparing your most excellent resume. 2) Follow job boards and check...letters that expound on bench skills and experience. Most Addgene PhD level job descriptions clearly describe...

...helix, fragmenting plasmid DNA, PCR product, or chromosome. One of the main causes of degradation during...endonuclease I encoded by the gene endA. Nowadays, the most common E. coli strains used for plasmid amplification...preparation stabilizing the plasmid, and still allowing most downstream processing. On the other hand, nuclease...where you plan to store it and for how long. The most common solution is to keep your plasmid at -20°C...

...alteration. Molecular cloning techniques are some of the most widely used techniques in the biological sciences...Although each type of cloning has its advantages, most scientists still encounter several struggles with... overhangs, insert and vector purification, and, most importantly, excessive time requirements. FastCloning...polymerases like phusion. An 18-cycle PCR leads to the most colonies after transformation. When a 1:1 vector...

...less waste.  Most communities seem to have recycling programs and it seems to me that most Addgenies make...advent of techniques like PCR and DNA synthesis. Most molecular biologists bought the pricey stuff in ...only Addgenie who has a thrifty streak.  One of the most accessed blogs on BitesizeBio is the protocol for...

...Scientist, Matt Ferenc, to organize the CRISPR forum's most popular questions and answers. Here are just 4 of...homology arms be for efficient recombination? A3: The most we've tried to insert so far has been 1kb. We used...background information on CRISPR/Cas9 systems Peruse the most recent genome editing review articles, such as: ...March 2014. Or browse the articles related to the most frequently requested CRISPR plasmids at Addgene ...

...advantage of that in your work The part of my job I most enjoy is building and telling interesting stories...following cause-and-effect relationships are all things almost all of us are programmed to appreciate and understand...had a conflict at work.” Interviewee: “I did the most work for a paper we were publishing, but when it...rethink your structure. Develop your protagonist In most cases, the protagonist will be you. Make sure to...

...podcasts, science pub nights, and pop sci books. Most scicomm efforts fall under informal learning; however...disease testing, it can still be considered jargon in most other uses. Many laypeople would use “PCR test” ... rather quickly!  The question   The first, and most important question, you should ask for any science...In fact, it’s really the opposite. Unlike emails, most science communication work does not explicitly exclude...

...exist either autonomously or integrated into the chromosome” was proposed a few years later by Élie Jacob... 1952 was noted to integrate into the E. coli chromosome in some cases. This terminology held until the...1960s when scientists began to study other extrachromosomal particles, particularly Resistance or R-factors... idea that R-factors could integrate into the chromosome. Thus the term “episome” was eventually dropped...

...sales and marketing jobs Like most choices we make, my job choices were mostly by chance. Luckily, around...but also my business acumen and sales experience. Most scientists think that they are unable to tick these...both my scientific and marketing knowledge. For most PhDs looking at careers outside of academia, sales...

... to correct only a minority of those variants in most cell types. But prime editing, a CRISPR technique...efficiency of HDR is relatively low, meaning that most Cas9-induced DSBs are repaired by non-homologous... end joining. As a result, Cas9 treatment causes most products to be indels while the efficiency of HDR...application, and the efficacy varies widely — it still almost always results in higher ratios of desired edits...binding site, increase processivity, or improve thermostability. What came out on top? The Cas9 nickase fused...

... stands for Fragmentation System and this is the most important part of this newly released prep kit. ...insulated glass to take home with us. Tips to to get the most out of a workshop Although we probably won’t use...the exact way we did at the workshop (the kit is mostly used to make new libraries as opposed to replicating...workshop because it will help you think about the most useful pieces of information from the workshop. ...

...Material Transfer Agreement (UBMTA) is one of the most commonly used ones for transfers between academic...research use agreements. The UBMTA, which is the MTA most plasmids at Addgene are shared under, is well-understood...the majority of research institutes.  Pro tip! In most academic and nonprofit institutes, the Tech Transfer... to share your biological materials under! While most plasmids at Addgene are shared under the UBMTA, ...

... is the most important resource that Addgene provides the scientific community? Katz: The most important...required to do research. Paratore: What I’ve been most impressed with is the level of proficiency that ...science? What about the future of open science are you most excited about? Katz: What’s exciting about the open...

... envelope expressing plasmid, such as our second most requested plasmid of all time, pMD2.G. Find psPAX2...this plasmid. Indeed the first version is the 15th most popular plasmid of all time, but was found to have...available through Addgene in 2015, making this the most recently available plasmid in the top 15. Find pSpCas9... your gRNA. It’s the former version of our sixth most popular plasmid pSpCas9(BB)-2A-Puro (PX459) V2.0...

...signaling molecules. Assay Type Westerns and ELISAs mostly use HRP, a chemiluminescent conjugate that can ...however, is pretty much every other assay you can do. Most non-HRP conjugates are fluorophores, which work ...be detected in the same assay. This is one of the most complex subjects in antibody-based protocols - but... them whenever possible. This will allow for the most accurate read and reduce needed compensation.    ...