...than four Maxipreps worth. Count colonies on the most dilute Petri dish. Total colony yield = count x ...in library. Example: At least 10 colonies on the most diluted plate for 1000x coverage of a library of... are present. If colonies are not present on the most dilute plate, count the second plate (ie. the 1:...tend to report lower values than Nanodrop, although most protocols have been designed with Nanodrop as the...

...been generated by restriction enzyme digestion . Most restriction enzymes digest DNA asymmetrically across...size of each and their concentration. However, for most standard cloning (where the insert is smaller than... + Background due to vector re-circularization - most useful for phosphatase treated vector Insert or ...

...so, for this protocol, we'll focus on one of the most common types that you're likely to encounter: the...properties of light to detect small objects. Two of the most important properties of a microscope are magnification... will see several pieces of information, but the most important (for now) is the magnification power, ...

...gently spread over the surface until the liquid is mostly absorbed (there is a very small visible volume ...gently spread over the surface until the liquid is mostly absorbed. The spreading of cells can be done in...

...following amendments: Notes: Gel purification is most efficient with lower % agarose gels, so you will...will want to isolate the DNA from the gel. This is most commonly done with a commercial gel purification...

...they recognize, restriction digests have become the most widely used method scientists employ to selectively...cut the plasmid into 3-8 pieces such that all (or most) fragments are small enough to be accurately sized...

...labels for the core parts that would be found on almost any centrifuge. Types and Parts There are several...techniques. Regardless of the type of centrifuge you use, most will have essentially the same components. The photo...

...Cloning? No need for specific restriction sites. Join almost any two fragments regardless of sequence. No scar...contiguous DNA fragment. Pro-Tip Add Extreme Thermostable Single-Stranded DNA-Binding protein (ET SSB)...

...contains the plasmid DNA separated from bacterial chromosomes. Collect the supernatant into a new tube by pipetting.... This is included in the resuspension buffer of most kits. Reference Page Top Index...

...green are the ones loaded onto the qPCR plate for most samples. If sample is expected to have a titer <...have some small amount of background signal that is most evident during initial PCR cycles. This background...

...Although our recommended temperature should kill most potential contaminants, certain types of spores ... agar will remain liquid at this temperature but most antibiotics will not break down at this temperature...

...is. While simple homogenization is effective for most mammalian tissues; more hardy tissues such as bone...

...different wavelengths of light to varying degrees and most have a specific wavelength that they maximally absorb...

...concentration of long-term glycerol storage is unknown. Most labs store bacteria in 15-25% glycerol. You can ...

...and room temperature. This general protocol suits almost any water bath. Video Watch the video for tips ...

...best buffer that works for both of your enzymes. Most companies will have a compatibility chart, such ...

...manufacturer’s instructions for your competent cells. For most standard cloning, you can transform 1-2μl of your...

... placing it in the PCR machine. Diluting Primers Most people order primers from a company, which synthesizes...

...of dilutions and pick the population that has the most desirable level of expression. Over time, transgene...

...manufacturer’s instructions for your competent cells. For most standard cloning, you can transform 1-2μl of your...