Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering
Type
Blog Post
Published
May 3, 2018, 1:48 p.m.
...cleavage promotes collateral cleavage of other RNAs. As with other Cas13s, this collateral cleavage does ...These small, but mighty type VI-D enzymes are the latest tools in the transcriptome engineering toolbox....identified other advantages, including Cas13d-specific regulatory proteins and high targeting efficiency, both ...accessory protein RspWYL1 increases both targeted and collateral RNA degradation by RspCas13d. RspWYL1 also increased...WYL domain-containing proteins may be broader regulators of Cas13d activity. This property makes WYL proteins...counterpart to anti-CRISPR proteins that negatively modulate the activity of Cas enzymes, some of which are...Konermann et al. compared CasRx to multiple RNA regulating methods: small hairpin RNA interference, dCas9...