... results at the bench, with a special focus on cloning and plasmid-based technologies. Commentary about...

...that have standard cloning sites or gateway compatible cloning sites for easy cloning of your genes of ... and repair templates of choice though modular cloning steps. The Voytas lab plans to update the toolkit...

... fluorescent proteins, synthetic biology, gene cloning and expression, as well as guide pages for Synthetic...

...ligands for different experiments without having to clone a whole new construct for every application. Plus...your labels easy! Instead of taking the time to clone a new construct for every new-and-improved fluorescent...able to get a plasmid ready-to-use without any cloning at all! As mentioned above, several variants of...

...naked eye. This makes cloning quite a bit easier since you can see if your cloning experiment worked just...

... by developing generic strategies for detecting cloned genes (Munro and Pelham, 1984). The strategy they...peptide tagging to detect proteins expressed from cloned genes: deletion mapping functional domains of Drosophila...

...H840. To ensure that the clones were capable of cleavage, they tested the clones against an NNN PAM library...

...Pathway Clone Collection 2.0. The collection is a set of vectors for use with the Gateway Cloning Platform...

...specific genomic locus, which can require multiple cloning steps (Fujita et al., 2013). CRISPR-mediated purification...can be targeted using a gRNA, which can easily be cloned into a gRNA expression plasmid. Experimental use...

...the 29 SARS-CoV-2 proteins which all but one are cloned into pLVX vectors (for lentiviral packaging) and...and contain an IRES-Puro marker for stable cell clone selection. For more information on these plasmids...

... follows a simple workflow: Clone spacer(s) into the CRISPR array Clone desired DNA cargo into the mini-transposon...

...If you’ve ever used Golden Gate Assembly for cloning, you might be familiar with the rules of thumb for...complexity allowable by a favored, fairly easy cloning technique (which may be of particular interest ...

... also loved helping to answer your plasmid and cloning questions through our Plasmids 101 series and by...

...demonstrated in human cells They used the Golden Gate cloning method to ligate expression cassettes of the gRNAs...

...., 1983).  We initiated research on expression cloning of novel oncogenes beyond ras and myc.  For this...

...molecular biology work has focused on using various cloning methods and tools to construct chimeric cellulases...

... al., 2017). Our vectors have two Bsa1 sites to clone the single guide RNA (protospacer+scaffold) in a...single polycistronic tRNA-gRNA (PTG) gene system to clone multiple protospacers which enables us to target...

...NNN) at any given codon site of interest can be cloned into an expression vector with Gibson assembly....Both produced about the same number of positive clones. See Supplementary Note 1 in Wrenbeck et al for...

...of E. coli for DNA propagation. While great for cloning purposes, these E. coli strains are not usually... (DE3)** Rifampicin RecA-deficient; allows for cloning and expression in same strain F- recA1 hsdR(rK12...

... To test the potential of Cas14, the Doudna lab cloned the cas14 gene together with the adjacent CRISPR...