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We narrowed to 228 results for: cloning

Showing: 121 - 140 of 228 results

  1. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    Published
    March 23, 2021, 1:15 p.m.
    ... about a week. Taking advantage of Golden Gate cloning, the Haynes lab’s Protocols.io protocol describes...useful vectors compatible with Gibson and Saptrap cloning systems to generate AID* tagged CRISPR knock-in...

  2. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    Published
    Oct. 4, 2016, 2:30 p.m.
    ...single cell clones for in vitro CRISPR experiments. The likelihood that a single cell clone derived from...may not be worth the cost and labor when single clones are selected. Conversely, translational research...

  3. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    Published
    Aug. 9, 2022, 1:15 p.m.
    ...that takes advantage of DNA sequencing to turn mAb clones into expression constructs to be conveniently modified...accuracy while screening. We also compare different clones of the same target to see if the results are the...

  4. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    Published
    May 26, 2016, 2:30 p.m.
    ...DNA left over for other applications, like PCR, cloning, transfection, or long-term storage. But what about...incomplete. Protein expression strains are not good for cloning Bacterial strains adapted for protein purification...

  5. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    Published
    May 29, 2014, 1:29 p.m.
    ...website to verify plasmids or validating your new clones, try these tips to make the most of your time and... to help other labs speed up their plasmid and cloning verification steps and free up more time for using...

  7. 15 Hot Plasmids from 2017

    Type
    Blog Post
    Published
    Jan. 9, 2018, 3:02 p.m.
    ...that have standard cloning sites or gateway compatible cloning sites for easy cloning of your genes of ... and repair templates of choice though modular cloning steps. The Voytas lab plans to update the toolkit...

  8. Introducing Addgene's Microbiology Pages

    Type
    Blog Post
    Published
    June 13, 2016, 7:08 p.m.
    ... fluorescent proteins, synthetic biology, gene cloning and expression, as well as guide pages for Synthetic...

  9. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    Published
    April 16, 2024, 1:15 p.m.
    ...ligands for different experiments without having to clone a whole new construct for every application. Plus...your labels easy! Instead of taking the time to clone a new construct for every new-and-improved fluorescent...able to get a plasmid ready-to-use without any cloning at all! As mentioned above, several variants of...

  12. Of Myc and Men

    Type
    Blog Post
    Published
    Jan. 19, 2023, 2:15 p.m.
    ... by developing generic strategies for detecting cloned genes (Munro and Pelham, 1984). The strategy they...peptide tagging to detect proteins expressed from cloned genes: deletion mapping functional domains of Drosophila...

  14. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    Published
    July 12, 2021, 1:15 p.m.
    ...the 29 SARS-CoV-2 proteins which all but one are cloned into pLVX vectors (for lentiviral packaging) and...and contain an IRES-Puro marker for stable cell clone selection. For more information on these plasmids...

  15. Isolating Genomic Regions of Interest with the CRISPR System

    Type
    Blog Post
    Published
    May 30, 2019, 12:44 p.m.
    ...specific genomic locus, which can require multiple cloning steps (Fujita et al., 2013). CRISPR-mediated purification...can be targeted using a gRNA, which can easily be cloned into a gRNA expression plasmid. Experimental use...

  16. High Complexity Golden Gate Assembly with NEB’s DAD

    Type
    Blog Post
    Published
    Aug. 1, 2023, 1:15 p.m.
    ...If you’ve ever used Golden Gate Assembly for cloning, you might be familiar with the rules of thumb for...complexity allowable by a favored, fairly easy cloning technique (which may be of particular interest ...

  18. Addgene’s Top 10 Blog Posts from 2014

    Type
    Blog Post
    Published
    Dec. 16, 2014, 8:01 p.m.
    ... also loved helping to answer your plasmid and cloning questions through our Plasmids 101 series and by...
Showing: 121 - 140 of 228 results