...whether I’m working with a talented journalist to shine light on living as a scientist in Beijing, talking...emotional investment and following cause-and-effect relationships are all things almost all of us are programmed...Interviewee: “I did the most work for a paper we were publishing, but when it came time to add author details ...

...new FP. Their aim - to red-shift the boundaries of FP technology by shifting the emission maxima toward...APOBEC-Cas9n-UGI-mediated activity edits out a frame-shift mutation within the mCherry reporter. Quantification...

...returned the MTA, Addgene can move forward with shipping the material to the requestor.  Why the MTA?  ...certainly frustrating to find out your plasmid hasn’t shipped because a form needs to be signed. But the truth... And that’s where MTAs, and similar agreements, shine.  These types of agreements allow for centralized...

...jumped at the chance. Here was an opportunity to see Shinya Yamanaka celebrate the tenth anniversary of his...clinical trial with iPSCs with his collaborator Takahashi in 2014 – just 8 years after the OSKM paper – ...panel of thought leaders in the field--including Shinya Yamanaka, George Q. Daley, Christine Mummery, Lorenz...

...FDA-approved). This is a big job, but under the leadership of Nevan Krogan at UCSF we’ve brought together...line, with a row of FedEx envelopes waiting to be shipped out of the Krogan lab at UCSF. Our team members...Foussard, and Dave Gordon) have been quite busy shipping and sending plasmids to all who request them -...

... for clear communication, fair credit through authorship, and acceptance of a diversity of work styles...stress the fact that “miscommunication, a lack of leadership, and inappropriate tools or writing approaches...that was probably lost during these weird times of shift working and social distancing. The take home message...

...however, it requires high doses. Requests have shifted toward the more efficient AAV-PHP.eB, developed...Evolutionary tree depicting the phylogenetic relationship of common research species. Among these species..., Kim, H.M., Rajendran, P.S., Tompkins, J.D., Shivkumar, K., Deverman, B.E., Gradinaru V. Systemic AAV...0799-7 Lawler, A. J., Ramamurthy, E., Brown, A. R., Shin, N., Kim, Y., Toong, N., Kaplow, I. M., Wirthlin....017 Matsuzaki, Y., Konno, A., Mochizuki, R., Shinohara, Y., Nitta, K., Okada, Y., & Hirai, H. (2018)....

...the US and Canada convene at ComSciCon's annual flagship workshop.  Our 2018 workshop took place in Boston...students, and we pay for travel expenses to our flagship workshop. In our view, the best possible outcome...is a co-founder of ComSciCon and Chair of its Leadership Team.  Nathan did his PhD in astronomy and works...

...launched the BioBuilder Educational Foundation in partnership with high school teachers to boost interest and...students include The BioBuilder High School Apprenticeship, preparing local (Massachusetts), economically...economically disadvantaged students for paid summer internships. A summer teacher professional development program...

...Cancer and the Immune System: Deciphering the Relationship” by our guest blogger Subhadra Jayaraman, a ... 113-117. PubMed PMID: 28225754. 6. Marumoto, Tomotoshi, et al. "Development of a novel mouse glioma model...Cancer and the Immune System: Deciphering the Relationship Resources on Addgene.org Browse Cancer Plasmids...

...?) As a result, I’d always end up stressed and rushing, no matter how hard I tried to build in extra time...especially on days when I was tired, stressed, or rushing. I would spend the time quietly running through... consider it this way: When you’re stressed or rushing, you’re much more likely to make a mistake. When...

...30082838. PubMed Central PMCID: PMC6078992. Nishimasu, Hiroshi, et al. "Engineered CRISPR-Cas9 nuclease ...which required only the di-nucleotide PAM NG (Nishimasu et al., 2018). The David Liu Lab similarly described...

...and target DNA. Nishimasu H, Ran FA, Hsu PD, Konermann S, Shehata SI, Dohmae N, Ishitani R, Zhang F, Nureki...OO, Barcena C, Hsu PD, Habib N, Gootenberg JS, Nishimasu H, Nureki O, Zhang F. Nature. 2015 Jan 29;517(...

...mCyRFP1 include a redshifted-emission spectrum compared to that of CyOFP1, a large stokes shift, long fluorescence...s properties. pBAD-LSSmCherry1 is a long Stokes shift variant, which could be compatible with two-photon... Ti-Sapphire lasers. pBAD-RDSmCherry1 is a red-shifted variant which, with further development, could ...

.... PubMed Central PMCID: PMC4517063. 3. Mizushima, N., Yoshimori, T., & Levine, B. (2010). Methods in Mammalian...Pho8 phosphatase activity or the molecular weight shift of Pho8 from its uncleaved to cleaved form is the...

...Therapeutics Graduate Program has a compulsory internship to be taken in an industry, regulatory, or clinical...many notable contributions in this area include publishing the book ‘How Economics Shapes Science’ and contributing...employ them, the diversity of trainee backgrounds, publishing from a young scientist’s perspective, and the...

...plate with every plasmid or as individual plasmids shipped as agar stabs. Iverson, et al. ACS Synth Bio. ...likelihood of creating a functional knock-out via frameshift and minimize off-target effects. The library ...Destination series, pCAG-Golden-Gate-Esp3I-Destination Takashi Yamamoto pcDNA-TAL-NC2, pCAGGS-TAL-NC2 Charles... et al. Cell Rep. 2016. PubMed PMID: 27052166 Nishimura K, et al. Nat. Methods 2009. PubMed PMID: 19915560... prototrophy, can also aid in designing self-establishing metabolically cooperating (SeMeCo) communities...

...FRET vectors. Murakoshi H, et al. Sci Rep. 2015. Pubmed PMID: 26469148. Murakoshi H, et al. Sci Rep...segment Article contributed by Christina Mork The Murakoshi lab has developed two new FLIM-FRET acceptors,...

...01039-7 Nishimasu, H., Ran, F. A., Hsu, P. D., Konermann, S., Shehata, S. I., Dohmae, N., Ishitani, R., ...disrupting its canonical structure and Cas9 binding (Nishimasu et al., 2014). Figure 3: pegRNAs whose...

...Similarly, if you want to knock out a gene, a frameshift mutation introduced anywhere within the early...with NG PAM compatibility, as the name implies (Nishimasu et. al, 2018). xCas9 was generated by the Liu ...10.1126/science.aba8853 (Plasmids available here)   Nishimasu, H., et al.,Engineered CRISPR-Cas9 nuclease with...