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  1. What's New in CRISPR - Spring 2019

    Type
    Blog Post
    Published
    March 21, 2019, 12:47 p.m.
    ...Plant base editing with a Cas9-adenosine deaminase fusion Caixia Gao’s lab has developed a new plant base...from an evolved tRNA adenosine deaminase that is fused to a nicking Cas9. This base editor can be used ...

  2. Designer PUF Proteins for Any RNA Target

    Type
    Blog Post
    Published
    June 17, 2014, 7:57 p.m.
    ... is fused to a translational repressor. In principle, however, designer PUF proteins can be fused to other...

  3. What's New in CRISPR - December 2019

    Type
    Blog Post
    Published
    Dec. 17, 2019, 2:37 p.m.
    ... and A-to-G base editors into one protein. They fused an activation-induced cytidine deaminase (AID) and...adenosine-to-inosine (A-to-I) RNA editor called REPAIR by fusing catalytically inactivated RNA-targeting Cas13 (...

  4. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    Published
    Nov. 19, 2013, 2:37 p.m.
    ... e67902. Many imaging studies rely on direct fusion of fluorescent proteins (FPs) to a yeast gene of...epitope tag for creation of your own unique protein fusions. This collection is ideal for tandem affinity purification...

  6. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    Published
    June 30, 2015, 2 p.m.
    ...The first functional SB system was synthesized by fusing and modifying various extinct transposases. Improvements...is made up of a fragment of the RPL13a promoter fused to a region of the RPL41 gene, to drive the selection...

  7. Another Pathway into Cells: iTOP

    Type
    Blog Post
    Published
    June 23, 2015, 8:37 p.m.
    ... common protein-only transduction strategy is to fuse a cell penetrating peptide (CPP) to a protein of...discovered in the HIV protein Tat. Although CPP fusions can translocate a cell membrane, the presence of...

  8. New and Upcoming Viral Vectors - June 2019

    Type
    Blog Post
    Published
    June 18, 2019, 1:15 p.m.
    ...useful for studies in non-human primates, where fusion fluorescent proteins can affect DREADD trafficking... (PV2527) 105679  AAV5 ppAAV-CKIIa-stGtACR1-FusionRed New tetracycline transactivators Plasmid...

  9. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    Published
    April 18, 2019, 12:53 p.m.
    ...in human cells (Ma et al., 2015). To do so, they fused a different fluorescent protein to various Cas9 ...Cas9 variants, SpCas9, NmCas9, and St1Cas9. These fusions localized to specific loci based on the guide RNA...

  11. DAP arrays for multiplex gene editing

    Type
    Blog Post
    Published
    July 7, 2022, 1:15 p.m.
    ...lab first thought to enable MBE by using Cas12a fused base editors (dCas12a-BE). However, the poor editing...efficiencies they found led them back towards Cas9 fused base editors (nCas9-BE), as Cas9 is mechanistically...

  12. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    Published
    Sept. 8, 2015, 2:30 p.m.
    ...with other pathways. Since these effectors are diffusible, they’re also not suitable for spatially limited...light-sensitive two-component system (TCS). Levskaya et al. fused a photosensory domain from cyanobacteria to a common...

  13. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    Published
    March 25, 2014, 3:15 p.m.
    ...not appropriate for mammalian selection – do not confuse this with G418 (aka Geneticin). Neomycin should...mammalian expression – instead use G418. This can be confusing since the neo/kan gene confers G418 resistance...

  14. Viral Vectors 101: Viruses as Biological Tools

    Type
    Blog Post
    Published
    March 21, 2023, 1:15 p.m.
    ...unit. Each particle has a viral envelope, which fuses with the target cell’s membrane, allowing the viral...determine what type of cell the virus will target and fuse with. In nature, this determines which organisms...

  15. Plasmids 101: Screens vs. Selections

    Type
    Blog Post
    Published
    Nov. 10, 2022, 2:15 p.m.
    ... Screens and selections The common goals The confusion in differentiating between a screen and a selection...inappropriately categorized as screens! Why the confusion? The commonality between these methods is that...

  16. Hot Plasmids - May 2022

    Type
    Blog Post
    Published
    May 26, 2022, 1:15 p.m.
    ...2021.    BLITZ uses the biotin ligase TurboID fused to a conditionally stabilized GFP-nanobody rather...rather than a specific protein of interest. This fusion protein can then be co-expressed in essentially any...

  17. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    Published
    April 5, 2022, 1:20 p.m.
    ...cryopreserved hybridomas, immortalized cells created by fusing an activated B cell that produces the antibody ...light (VL) and variable heavy (VH) chain sequences. Fusion PCR was performed to create an amplicon that  was...

  19. To Each HIS Own

    Type
    Blog Post
    Published
    March 2, 2023, 2:15 p.m.
    ...   Prior to the polyhistidine tag, alternative fusion tags were used, such as S. aureus’ Protein A (Moks... to scientists. If their protein of interest is fused to polyhistidine, then they can use an anti-HIS ...

  20. Developing Lab Management Software for Biology

    Type
    Blog Post
    Published
    Aug. 25, 2016, 2:30 p.m.
    ... as clear as you can be (there will always be confusion about this though) about the fact that containers...them to their contents might leave you sad and confused when a tricky procedure failed and one of your...
Showing: 121 - 140 of 279 results