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  2. Protocol - How to Ligate Plasmid DNA

    Type
    Protocol
    ...the two pieces of DNA connect and ultimately are fused by the ligation reaction. The example below depicts...therefore prevents the ligase from being able to fuse the two ends of the vector together). Protocol: ...

  3. Pouring LB Agar Plates

    Type
    Protocol
    ...and the bottle contents. This will clear up any confusion later if your forget your bottle in the autoclave...likely that you’ve used the wrong antibiotic, or confused your strains....

  4. Plasmid Cloning by PCR (with Protocols)

    Type
    Protocol
    ...in the recipient plasmid by viewing the MCS and fusing the upstream restriction site to the forward primer...Insert Into Your Vector Conduct a DNA Ligation to fuse your insert to your recipient plasmid. We recommend...

  6. Ligation Independent Cloning

    Type
    Protocol
    ...backbone manufacturer's suggested leader sequence fused to your gene of interest, in frame with the start...
Showing: 1 - 8 of 8 results