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  7. Hot Plasmids February 2024

    Type
    Blog Post
    Published
    Feb. 20, 2024, 2:45 p.m.
    ...by co-incubation of Cas-RNP and assist peptide. Adapted from Zhang et al. 2023 with permission.   Find...

  11. Plasmids 101: Protein Expression

    Type
    Blog Post
    Published
    June 7, 2018, 1:17 p.m.
    ... grown in well-defined media and can be easily adapted to fermentation allowing for large-scale, stable...

  12. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    Published
    April 18, 2024, 1:15 p.m.
    ...our requestors. However, we can again see quick adaptation and steady use of all types of libraries, once...

  13. Plasmids 101: Degron Tags

    Type
    Blog Post
    Published
    March 14, 2024, 1:15 p.m.
    ...to ubiquitination and proteasomal degradation. Adapted from Nabet et al., Chemical Biology, 2018.  ...

  14. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    Published
    Jan. 30, 2024, 2:15 p.m.
    ...of the previously described systems. Several adaptations of this method have been to tag, cage, uncage...

  16. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    Published
    May 26, 2016, 2:30 p.m.
    ...strains are not good for cloning Bacterial strains adapted for protein purification use the cell’s resources...

  17. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    Published
    Sept. 9, 2014, 8:20 p.m.
    ...disadvantages of the IRES element, scientists have adapted "self-cleaving" 2A peptides into their muliticistronic...

  19. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    Published
    July 31, 2020, 12:30 p.m.
    ..., 2016) as a RNA-targeting CRISPR enzyme, they adapted Cas13b for precise RNA editing (Cox et al., 2017...

  20. DNA Purification Without a Kit

    Type
    Blog Post
    Published
    Feb. 11, 2020, 2:15 p.m.
    ...or TE. The Bishop Lab’s glass milk protocol is adapted from the 1979 Patterson Protocol. When published...
Showing: 121 - 140 of 178 results